Strain Fitness in Escherichia coli ECRC62 around BNILDI_01020

Experiment: Bas61

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcca and bacA are separated by 162 nucleotidesbacA and folB are separated by 90 nucleotidesfolB and plsY are separated by 104 nucleotides BNILDI_01015: cca - fused tRNA nucleotidyltransferase/2',3'-cyclic phosphodiesterase/2' nucleotidase/phosphatase Cca, at 195,551 to 196,789 cca BNILDI_01020: bacA - undecaprenyl-diphosphate phosphatase, at 196,952 to 197,773 bacA BNILDI_01025: folB - bifunctional dihydroneopterin aldolase/7,8-dihydroneopterin epimerase, at 197,864 to 198,232 folB BNILDI_01030: plsY - glycerol-3-phosphate 1-O-acyltransferase PlsY, at 198,337 to 198,954 plsY Position (kb) 196 197 198Strain fitness (log2 ratio) -1 0 1 2at 196.794 kb on - strandat 196.794 kb on - strandat 196.799 kb on + strandat 196.968 kb on + strandat 196.987 kb on + strandat 196.988 kb on - strandat 197.047 kb on + strand, within bacAat 197.048 kb on - strand, within bacAat 197.081 kb on - strand, within bacAat 197.081 kb on - strand, within bacAat 197.137 kb on - strand, within bacAat 197.300 kb on - strand, within bacAat 197.320 kb on + strand, within bacAat 197.404 kb on - strand, within bacAat 197.592 kb on - strand, within bacAat 197.612 kb on + strand, within bacAat 197.677 kb on + strand, within bacAat 197.678 kb on - strand, within bacAat 197.683 kb on + strand, within bacAat 197.684 kb on - strand, within bacAat 197.684 kb on - strand, within bacAat 197.738 kb on + strandat 197.777 kb on + strandat 197.777 kb on + strandat 197.790 kb on + strandat 197.790 kb on + strandat 197.791 kb on - strandat 197.838 kb on - strandat 198.287 kb on - strandat 198.336 kb on + strandat 198.336 kb on + strandat 198.337 kb on - strandat 198.337 kb on - strandat 198.476 kb on + strand, within plsYat 198.477 kb on - strand, within plsYat 198.564 kb on - strand, within plsYat 198.591 kb on - strand, within plsYat 198.740 kb on + strand, within plsY

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas61
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196,794 - -0.7
196,794 - -0.9
196,799 + +0.9
196,968 + -0.3
196,987 + -0.6
196,988 - -0.3
197,047 + bacA BNILDI_01020 0.12 -1.2
197,048 - bacA BNILDI_01020 0.12 +0.9
197,081 - bacA BNILDI_01020 0.16 -0.8
197,081 - bacA BNILDI_01020 0.16 -0.8
197,137 - bacA BNILDI_01020 0.23 -0.2
197,300 - bacA BNILDI_01020 0.42 -0.7
197,320 + bacA BNILDI_01020 0.45 -0.9
197,404 - bacA BNILDI_01020 0.55 -0.3
197,592 - bacA BNILDI_01020 0.78 +0.4
197,612 + bacA BNILDI_01020 0.80 -0.4
197,677 + bacA BNILDI_01020 0.88 +0.6
197,678 - bacA BNILDI_01020 0.88 +1.9
197,683 + bacA BNILDI_01020 0.89 +0.3
197,684 - bacA BNILDI_01020 0.89 -0.3
197,684 - bacA BNILDI_01020 0.89 +0.3
197,738 + +0.4
197,777 + +0.4
197,777 + +0.8
197,790 + +1.0
197,790 + -1.3
197,791 - +0.2
197,838 - -0.3
198,287 - -0.4
198,336 + -0.5
198,336 + +0.9
198,337 - +0.5
198,337 - -0.7
198,476 + plsY BNILDI_01030 0.22 -0.6
198,477 - plsY BNILDI_01030 0.23 -1.3
198,564 - plsY BNILDI_01030 0.37 -0.1
198,591 - plsY BNILDI_01030 0.41 -0.2
198,740 + plsY BNILDI_01030 0.65 -0.5

Or see this region's nucleotide sequence