Strain Fitness in Escherichia coli ECRC62 around BNILDI_00405

Experiment: Bas61

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttnp-ISAs1 and BNILDI_00400 are separated by 353 nucleotidesBNILDI_00400 and BNILDI_00405 are separated by 11 nucleotidesBNILDI_00405 and tssB are separated by 830 nucleotides BNILDI_00395: tnp-ISAs1 - ISAs1 family transposase, at 80,892 to 81,935 tnp-ISAs1 BNILDI_00400: BNILDI_00400 - DsORF-h1, at 82,289 to 82,738 _00400 BNILDI_00405: BNILDI_00405 - contractile injection system protein, VgrG/Pvc8 family, at 82,750 to 83,670 _00405 BNILDI_00410: tssB - Type VI secretion system protein TssB, at 84,501 to 85,001 tssB Position (kb) 82 83 84Strain fitness (log2 ratio) -1 0 1 2at 82.290 kb on + strandat 82.729 kb on - strandat 83.108 kb on + strand, within BNILDI_00405at 83.108 kb on + strand, within BNILDI_00405at 83.322 kb on + strand, within BNILDI_00405at 83.324 kb on + strand, within BNILDI_00405at 83.346 kb on - strand, within BNILDI_00405at 83.346 kb on - strand, within BNILDI_00405at 83.363 kb on + strand, within BNILDI_00405at 83.363 kb on + strand, within BNILDI_00405at 83.364 kb on - strand, within BNILDI_00405at 83.387 kb on + strand, within BNILDI_00405at 83.624 kb on + strandat 83.624 kb on + strandat 83.633 kb on + strandat 83.634 kb on - strandat 84.545 kb on + strandat 84.548 kb on + strandat 84.548 kb on + strandat 84.549 kb on - strandat 84.632 kb on + strand, within tssB

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas61
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82,290 + +0.0
82,729 - +0.3
83,108 + BNILDI_00405 0.39 +0.9
83,108 + BNILDI_00405 0.39 +0.0
83,322 + BNILDI_00405 0.62 +0.3
83,324 + BNILDI_00405 0.62 -0.2
83,346 - BNILDI_00405 0.65 +0.8
83,346 - BNILDI_00405 0.65 +0.9
83,363 + BNILDI_00405 0.67 +0.2
83,363 + BNILDI_00405 0.67 +0.5
83,364 - BNILDI_00405 0.67 -0.2
83,387 + BNILDI_00405 0.69 +1.0
83,624 + -0.4
83,624 + -1.1
83,633 + +0.9
83,634 - -1.3
84,545 + -1.1
84,548 + -0.3
84,548 + -0.4
84,549 - +0.1
84,632 + tssB BNILDI_00410 0.26 +1.9

Or see this region's nucleotide sequence