Strain Fitness in Escherichia coli ECRC62 around BNILDI_00275

Experiment: Bas61

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpepD and prfH are separated by 56 nucleotidesprfH and rtcB overlap by 4 nucleotides BNILDI_00270: pepD - cytosol nonspecific dipeptidase, at 59,219 to 60,676 pepD BNILDI_00275: prfH - peptide chain release factor H, at 60,733 to 61,347 prfH BNILDI_00280: rtcB - RNA ligase RtcB family protein, at 61,344 to 62,483 rtcB Position (kb) 60 61 62Strain fitness (log2 ratio) -2 -1 0 1 2at 59.748 kb on - strand, within pepDat 59.793 kb on + strand, within pepDat 59.841 kb on + strand, within pepDat 59.841 kb on + strand, within pepDat 59.842 kb on - strand, within pepDat 59.842 kb on - strand, within pepDat 59.894 kb on - strand, within pepDat 60.061 kb on + strand, within pepDat 60.062 kb on - strand, within pepDat 60.066 kb on - strand, within pepDat 60.066 kb on - strand, within pepDat 60.066 kb on - strand, within pepDat 60.066 kb on - strand, within pepDat 60.143 kb on - strand, within pepDat 60.173 kb on - strand, within pepDat 60.173 kb on - strand, within pepDat 60.287 kb on - strand, within pepDat 60.292 kb on - strand, within pepDat 60.292 kb on - strand, within pepDat 60.339 kb on - strand, within pepDat 60.372 kb on + strand, within pepDat 60.373 kb on - strand, within pepDat 60.405 kb on - strand, within pepDat 60.451 kb on - strand, within pepDat 60.462 kb on - strand, within pepDat 60.630 kb on - strandat 60.675 kb on - strandat 60.675 kb on - strandat 60.679 kb on - strandat 60.904 kb on - strand, within prfHat 60.973 kb on - strand, within prfHat 61.128 kb on + strand, within prfHat 61.129 kb on - strand, within prfHat 61.270 kb on + strand, within prfHat 61.411 kb on - strandat 61.411 kb on - strandat 61.455 kb on - strandat 61.455 kb on - strandat 61.589 kb on + strand, within rtcBat 61.635 kb on - strand, within rtcBat 61.645 kb on - strand, within rtcBat 61.657 kb on + strand, within rtcBat 61.658 kb on - strand, within rtcBat 61.658 kb on - strand, within rtcBat 61.680 kb on - strand, within rtcBat 61.815 kb on + strand, within rtcBat 61.844 kb on + strand, within rtcBat 61.881 kb on - strand, within rtcBat 61.882 kb on + strand, within rtcBat 61.882 kb on + strand, within rtcBat 61.883 kb on - strand, within rtcBat 61.948 kb on - strand, within rtcBat 61.979 kb on + strand, within rtcBat 62.213 kb on - strand, within rtcBat 62.228 kb on + strand, within rtcBat 62.341 kb on - strand, within rtcB

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas61
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59,748 - pepD BNILDI_00270 0.36 +0.6
59,793 + pepD BNILDI_00270 0.39 -0.1
59,841 + pepD BNILDI_00270 0.43 -0.8
59,841 + pepD BNILDI_00270 0.43 -0.4
59,842 - pepD BNILDI_00270 0.43 -0.7
59,842 - pepD BNILDI_00270 0.43 +1.9
59,894 - pepD BNILDI_00270 0.46 -0.3
60,061 + pepD BNILDI_00270 0.58 -1.8
60,062 - pepD BNILDI_00270 0.58 -0.7
60,066 - pepD BNILDI_00270 0.58 -0.7
60,066 - pepD BNILDI_00270 0.58 +1.2
60,066 - pepD BNILDI_00270 0.58 +0.6
60,066 - pepD BNILDI_00270 0.58 +0.9
60,143 - pepD BNILDI_00270 0.63 -0.2
60,173 - pepD BNILDI_00270 0.65 -0.4
60,173 - pepD BNILDI_00270 0.65 +1.5
60,287 - pepD BNILDI_00270 0.73 +0.3
60,292 - pepD BNILDI_00270 0.74 -1.4
60,292 - pepD BNILDI_00270 0.74 -2.3
60,339 - pepD BNILDI_00270 0.77 +0.6
60,372 + pepD BNILDI_00270 0.79 -0.6
60,373 - pepD BNILDI_00270 0.79 +0.0
60,405 - pepD BNILDI_00270 0.81 +1.3
60,451 - pepD BNILDI_00270 0.84 +0.6
60,462 - pepD BNILDI_00270 0.85 -0.9
60,630 - -0.1
60,675 - -1.0
60,675 - +0.1
60,679 - -0.6
60,904 - prfH BNILDI_00275 0.28 +0.8
60,973 - prfH BNILDI_00275 0.39 +0.3
61,128 + prfH BNILDI_00275 0.64 +0.7
61,129 - prfH BNILDI_00275 0.64 -0.8
61,270 + prfH BNILDI_00275 0.87 +1.0
61,411 - -0.1
61,411 - +0.9
61,455 - +1.5
61,455 - +0.7
61,589 + rtcB BNILDI_00280 0.21 -0.1
61,635 - rtcB BNILDI_00280 0.26 +0.1
61,645 - rtcB BNILDI_00280 0.26 +0.1
61,657 + rtcB BNILDI_00280 0.27 -0.1
61,658 - rtcB BNILDI_00280 0.28 -0.4
61,658 - rtcB BNILDI_00280 0.28 -0.4
61,680 - rtcB BNILDI_00280 0.29 +0.3
61,815 + rtcB BNILDI_00280 0.41 +0.3
61,844 + rtcB BNILDI_00280 0.44 +0.2
61,881 - rtcB BNILDI_00280 0.47 +0.3
61,882 + rtcB BNILDI_00280 0.47 -0.0
61,882 + rtcB BNILDI_00280 0.47 +0.9
61,883 - rtcB BNILDI_00280 0.47 +0.1
61,948 - rtcB BNILDI_00280 0.53 +0.2
61,979 + rtcB BNILDI_00280 0.56 +0.8
62,213 - rtcB BNILDI_00280 0.76 -1.4
62,228 + rtcB BNILDI_00280 0.78 -0.7
62,341 - rtcB BNILDI_00280 0.87 -0.9

Or see this region's nucleotide sequence