Strain Fitness in Escherichia coli ECRC62 around BNILDI_00030

Experiment: Bas61

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntbetA and ykgH are separated by 212 nucleotidesykgH and lutC are separated by 242 nucleotideslutC and ykgF overlap by 8 nucleotides BNILDI_00025: betA - choline dehydrogenase, at 7,265 to 8,935 betA BNILDI_00030: ykgH - Uncharacterized protein YkgH, at 9,148 to 9,816 ykgH BNILDI_00035: lutC - lactate utilization protein C, at 10,059 to 10,754 lutC BNILDI_00040: ykgF - LutB/LldF family L-lactate oxidation iron-sulfur protein, at 10,747 to 12,174 ykgF Position (kb) 9 10Strain fitness (log2 ratio) -2 -1 0 1 2at 8.171 kb on + strand, within betAat 8.171 kb on + strand, within betAat 8.172 kb on - strand, within betAat 8.172 kb on - strand, within betAat 8.172 kb on - strand, within betAat 8.375 kb on + strand, within betAat 8.504 kb on + strand, within betAat 8.504 kb on + strand, within betAat 8.505 kb on - strand, within betAat 8.505 kb on - strand, within betAat 8.505 kb on - strand, within betAat 8.505 kb on - strand, within betAat 8.827 kb on + strandat 8.828 kb on - strandat 8.845 kb on - strandat 8.894 kb on + strandat 8.979 kb on - strandat 9.007 kb on + strandat 9.008 kb on - strandat 9.269 kb on - strand, within ykgHat 9.340 kb on - strand, within ykgHat 9.349 kb on - strand, within ykgHat 9.845 kb on + strandat 9.916 kb on - strandat 9.931 kb on - strandat 9.933 kb on + strandat 9.945 kb on - strandat 10.019 kb on + strandat 10.052 kb on - strandat 10.082 kb on - strandat 10.122 kb on - strandat 10.250 kb on + strand, within lutCat 10.357 kb on + strand, within lutCat 10.358 kb on - strand, within lutCat 10.374 kb on - strand, within lutCat 10.583 kb on + strand, within lutCat 10.584 kb on - strand, within lutCat 10.631 kb on + strand, within lutCat 10.640 kb on + strand, within lutCat 10.657 kb on + strand, within lutCat 10.657 kb on + strand, within lutCat 10.657 kb on + strand, within lutC

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas61
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8,171 + betA BNILDI_00025 0.54 -0.7
8,171 + betA BNILDI_00025 0.54 -0.4
8,172 - betA BNILDI_00025 0.54 -0.2
8,172 - betA BNILDI_00025 0.54 +1.3
8,172 - betA BNILDI_00025 0.54 +1.8
8,375 + betA BNILDI_00025 0.66 -1.0
8,504 + betA BNILDI_00025 0.74 +0.6
8,504 + betA BNILDI_00025 0.74 -0.2
8,505 - betA BNILDI_00025 0.74 +0.4
8,505 - betA BNILDI_00025 0.74 +0.5
8,505 - betA BNILDI_00025 0.74 -0.2
8,505 - betA BNILDI_00025 0.74 -0.2
8,827 + +1.9
8,828 - -1.3
8,845 - +0.3
8,894 + +0.5
8,979 - +0.0
9,007 + -1.4
9,008 - +1.0
9,269 - ykgH BNILDI_00030 0.18 +0.7
9,340 - ykgH BNILDI_00030 0.29 +0.7
9,349 - ykgH BNILDI_00030 0.30 +1.5
9,845 + -0.8
9,916 - +1.2
9,931 - +0.3
9,933 + -2.2
9,945 - -1.0
10,019 + -0.2
10,052 - -0.2
10,082 - +0.1
10,122 - -0.2
10,250 + lutC BNILDI_00035 0.27 +0.7
10,357 + lutC BNILDI_00035 0.43 +1.0
10,358 - lutC BNILDI_00035 0.43 -0.5
10,374 - lutC BNILDI_00035 0.45 +0.7
10,583 + lutC BNILDI_00035 0.75 +2.7
10,584 - lutC BNILDI_00035 0.75 +0.2
10,631 + lutC BNILDI_00035 0.82 +0.5
10,640 + lutC BNILDI_00035 0.83 -0.8
10,657 + lutC BNILDI_00035 0.86 -0.6
10,657 + lutC BNILDI_00035 0.86 +0.2
10,657 + lutC BNILDI_00035 0.86 -1.0

Or see this region's nucleotide sequence