Experiment: Bas48
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt eutR and hemF are separated by 5 nucleotides hemF and amiA are separated by 0 nucleotides amiA and ypeA are separated by 213 nucleotides ypeA and yfeZ overlap by 14 nucleotides
HEPCGN_21265: eutR - HTH-type transcriptional regulator EutR, at 3,006,743 to 3,007,795
eutR
HEPCGN_21270: hemF - oxygen-dependent coproporphyrinogen oxidase, at 3,007,801 to 3,008,703
hemF
HEPCGN_21275: amiA - N-acetylmuramoyl-L-alanine amidase AmiA, at 3,008,704 to 3,009,573
amiA
HEPCGN_21280: ypeA - GNAT family acetyltransferase, at 3,009,787 to 3,010,212
ypeA
HEPCGN_21285: yfeZ - Inner membrane protein YfeZ, at 3,010,199 to 3,010,648
yfeZ
Position (kb)
3008
3009
3010 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3007.802 kb on + strand at 3007.825 kb on - strand at 3007.825 kb on - strand at 3007.835 kb on - strand at 3007.875 kb on - strand at 3007.911 kb on + strand, within hemF at 3007.912 kb on - strand, within hemF at 3007.997 kb on + strand, within hemF at 3007.999 kb on + strand, within hemF at 3007.999 kb on + strand, within hemF at 3007.999 kb on + strand, within hemF at 3008.000 kb on - strand, within hemF at 3008.041 kb on - strand, within hemF at 3008.051 kb on - strand, within hemF at 3008.097 kb on + strand, within hemF at 3008.098 kb on - strand, within hemF at 3008.213 kb on + strand, within hemF at 3008.298 kb on - strand, within hemF at 3008.399 kb on + strand, within hemF at 3008.400 kb on - strand, within hemF at 3008.598 kb on + strand, within hemF at 3008.705 kb on + strand at 3008.750 kb on + strand at 3008.751 kb on - strand at 3008.751 kb on - strand at 3008.952 kb on + strand, within amiA at 3008.979 kb on + strand, within amiA at 3009.022 kb on + strand, within amiA at 3009.023 kb on - strand, within amiA at 3009.023 kb on - strand, within amiA at 3009.089 kb on + strand, within amiA at 3009.108 kb on + strand, within amiA at 3009.163 kb on - strand, within amiA at 3009.186 kb on + strand, within amiA at 3009.186 kb on + strand, within amiA at 3009.187 kb on - strand, within amiA at 3009.230 kb on + strand, within amiA at 3009.231 kb on - strand, within amiA at 3009.233 kb on - strand, within amiA at 3009.241 kb on - strand, within amiA at 3009.310 kb on - strand, within amiA at 3009.553 kb on + strand at 3009.562 kb on - strand at 3009.587 kb on + strand at 3009.593 kb on + strand at 3009.608 kb on + strand at 3009.609 kb on - strand at 3009.609 kb on - strand at 3009.611 kb on - strand at 3009.658 kb on + strand at 3009.729 kb on + strand at 3009.750 kb on - strand at 3009.794 kb on + strand at 3009.794 kb on + strand at 3009.894 kb on + strand, within ypeA at 3009.895 kb on - strand, within ypeA at 3009.895 kb on - strand, within ypeA at 3009.994 kb on + strand, within ypeA at 3009.997 kb on + strand, within ypeA at 3009.997 kb on + strand, within ypeA at 3010.039 kb on - strand, within ypeA at 3010.116 kb on + strand, within ypeA at 3010.139 kb on - strand, within ypeA at 3010.139 kb on - strand, within ypeA at 3010.139 kb on - strand, within ypeA at 3010.207 kb on + strand at 3010.229 kb on + strand at 3010.229 kb on + strand at 3010.279 kb on - strand, within yfeZ
Per-strain Table
Position Strand Gene LocusTag Fraction Bas48 remove 3,007,802 + -0.6 3,007,825 - -0.0 3,007,825 - -1.5 3,007,835 - +0.1 3,007,875 - -0.1 3,007,911 + hemF HEPCGN_21270 0.12 +0.1 3,007,912 - hemF HEPCGN_21270 0.12 -0.0 3,007,997 + hemF HEPCGN_21270 0.22 +0.5 3,007,999 + hemF HEPCGN_21270 0.22 +0.0 3,007,999 + hemF HEPCGN_21270 0.22 +0.5 3,007,999 + hemF HEPCGN_21270 0.22 -0.7 3,008,000 - hemF HEPCGN_21270 0.22 -0.3 3,008,041 - hemF HEPCGN_21270 0.27 +0.4 3,008,051 - hemF HEPCGN_21270 0.28 +0.2 3,008,097 + hemF HEPCGN_21270 0.33 +2.3 3,008,098 - hemF HEPCGN_21270 0.33 -0.5 3,008,213 + hemF HEPCGN_21270 0.46 -0.5 3,008,298 - hemF HEPCGN_21270 0.55 -1.7 3,008,399 + hemF HEPCGN_21270 0.66 +0.5 3,008,400 - hemF HEPCGN_21270 0.66 +0.5 3,008,598 + hemF HEPCGN_21270 0.88 -0.3 3,008,705 + +1.6 3,008,750 + -2.4 3,008,751 - -1.0 3,008,751 - -1.5 3,008,952 + amiA HEPCGN_21275 0.29 +1.1 3,008,979 + amiA HEPCGN_21275 0.32 -1.8 3,009,022 + amiA HEPCGN_21275 0.37 -3.0 3,009,023 - amiA HEPCGN_21275 0.37 -1.6 3,009,023 - amiA HEPCGN_21275 0.37 +0.3 3,009,089 + amiA HEPCGN_21275 0.44 -2.0 3,009,108 + amiA HEPCGN_21275 0.46 -1.2 3,009,163 - amiA HEPCGN_21275 0.53 -3.8 3,009,186 + amiA HEPCGN_21275 0.55 -1.8 3,009,186 + amiA HEPCGN_21275 0.55 -2.1 3,009,187 - amiA HEPCGN_21275 0.56 -4.3 3,009,230 + amiA HEPCGN_21275 0.60 -1.3 3,009,231 - amiA HEPCGN_21275 0.61 -0.8 3,009,233 - amiA HEPCGN_21275 0.61 +0.5 3,009,241 - amiA HEPCGN_21275 0.62 -1.4 3,009,310 - amiA HEPCGN_21275 0.70 +0.1 3,009,553 + -1.9 3,009,562 - -2.0 3,009,587 + -2.6 3,009,593 + -0.1 3,009,608 + -1.3 3,009,609 - +0.6 3,009,609 - -0.2 3,009,611 - +0.7 3,009,658 + -0.7 3,009,729 + +1.1 3,009,750 - -1.1 3,009,794 + +0.3 3,009,794 + -0.9 3,009,894 + ypeA HEPCGN_21280 0.25 -0.6 3,009,895 - ypeA HEPCGN_21280 0.25 -0.4 3,009,895 - ypeA HEPCGN_21280 0.25 +0.5 3,009,994 + ypeA HEPCGN_21280 0.49 -0.5 3,009,997 + ypeA HEPCGN_21280 0.49 -0.6 3,009,997 + ypeA HEPCGN_21280 0.49 +0.3 3,010,039 - ypeA HEPCGN_21280 0.59 -0.6 3,010,116 + ypeA HEPCGN_21280 0.77 +0.9 3,010,139 - ypeA HEPCGN_21280 0.83 -0.5 3,010,139 - ypeA HEPCGN_21280 0.83 -0.4 3,010,139 - ypeA HEPCGN_21280 0.83 -1.4 3,010,207 + -1.2 3,010,229 + +0.1 3,010,229 + +0.2 3,010,279 - yfeZ HEPCGN_21285 0.18 -0.4
Or see this region's nucleotide sequence