Experiment: Bas48
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mutL and amiB are separated by 9 nucleotides amiB and tsaE are separated by 18 nucleotides tsaE and nnr overlap by 29 nucleotides
HEPCGN_10495: mutL - DNA mismatch repair endonuclease MutL, at 840,802 to 842,649
mutL
HEPCGN_10500: amiB - N-acetylmuramoyl-L-alanine amidase AmiB, at 842,659 to 843,996
amiB
HEPCGN_10505: tsaE - tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE, at 844,015 to 844,476
tsaE
HEPCGN_10510: nnr - bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase, at 844,448 to 845,980
nnr
Position (kb) 842
843
844 Strain fitness (log2 ratio) -2
-1
0
1
2 at 841.682 kb on + strand, within mutL at 841.779 kb on - strand, within mutL at 841.880 kb on + strand, within mutL at 841.881 kb on - strand, within mutL at 841.886 kb on + strand, within mutL at 842.145 kb on + strand, within mutL at 842.195 kb on + strand, within mutL at 842.196 kb on - strand, within mutL at 842.196 kb on - strand, within mutL at 842.196 kb on - strand, within mutL at 842.247 kb on + strand, within mutL at 842.416 kb on - strand, within mutL at 842.491 kb on - strand at 842.491 kb on - strand at 842.673 kb on + strand at 842.673 kb on + strand at 842.771 kb on + strand at 842.860 kb on + strand, within amiB at 842.955 kb on + strand, within amiB at 842.955 kb on + strand, within amiB at 842.955 kb on + strand, within amiB at 842.956 kb on - strand, within amiB at 842.966 kb on - strand, within amiB at 842.966 kb on - strand, within amiB at 842.973 kb on - strand, within amiB at 842.973 kb on - strand, within amiB at 843.002 kb on - strand, within amiB at 843.073 kb on + strand, within amiB at 843.222 kb on + strand, within amiB at 843.261 kb on - strand, within amiB at 843.315 kb on + strand, within amiB at 843.316 kb on - strand, within amiB at 843.319 kb on + strand, within amiB at 843.320 kb on - strand, within amiB at 843.320 kb on - strand, within amiB at 843.472 kb on - strand, within amiB at 843.474 kb on + strand, within amiB at 843.484 kb on - strand, within amiB at 843.493 kb on - strand, within amiB at 843.496 kb on - strand, within amiB at 843.496 kb on - strand, within amiB at 843.641 kb on + strand, within amiB at 843.641 kb on + strand, within amiB at 843.642 kb on - strand, within amiB at 843.642 kb on - strand, within amiB at 843.990 kb on - strand at 843.990 kb on - strand at 843.990 kb on - strand at 843.990 kb on - strand at 843.990 kb on - strand at 844.484 kb on - strand at 844.620 kb on + strand, within nnr at 844.657 kb on + strand, within nnr at 844.791 kb on - strand, within nnr at 844.811 kb on + strand, within nnr at 844.812 kb on - strand, within nnr
Per-strain Table
Position Strand Gene LocusTag Fraction Bas48 remove 841,682 + mutL HEPCGN_10495 0.48 -1.9 841,779 - mutL HEPCGN_10495 0.53 +0.4 841,880 + mutL HEPCGN_10495 0.58 -0.4 841,881 - mutL HEPCGN_10495 0.58 -0.1 841,886 + mutL HEPCGN_10495 0.59 -0.4 842,145 + mutL HEPCGN_10495 0.73 -0.2 842,195 + mutL HEPCGN_10495 0.75 -0.9 842,196 - mutL HEPCGN_10495 0.75 -0.1 842,196 - mutL HEPCGN_10495 0.75 +0.1 842,196 - mutL HEPCGN_10495 0.75 -1.3 842,247 + mutL HEPCGN_10495 0.78 -0.8 842,416 - mutL HEPCGN_10495 0.87 +0.9 842,491 - -0.5 842,491 - -0.6 842,673 + -0.8 842,673 + +0.4 842,771 + -0.1 842,860 + amiB HEPCGN_10500 0.15 -2.0 842,955 + amiB HEPCGN_10500 0.22 -1.2 842,955 + amiB HEPCGN_10500 0.22 -0.6 842,955 + amiB HEPCGN_10500 0.22 +0.4 842,956 - amiB HEPCGN_10500 0.22 -1.2 842,966 - amiB HEPCGN_10500 0.23 -0.1 842,966 - amiB HEPCGN_10500 0.23 -0.5 842,973 - amiB HEPCGN_10500 0.23 +0.5 842,973 - amiB HEPCGN_10500 0.23 -0.7 843,002 - amiB HEPCGN_10500 0.26 -1.6 843,073 + amiB HEPCGN_10500 0.31 -0.6 843,222 + amiB HEPCGN_10500 0.42 -0.6 843,261 - amiB HEPCGN_10500 0.45 +2.4 843,315 + amiB HEPCGN_10500 0.49 -0.2 843,316 - amiB HEPCGN_10500 0.49 -0.2 843,319 + amiB HEPCGN_10500 0.49 -0.7 843,320 - amiB HEPCGN_10500 0.49 +1.0 843,320 - amiB HEPCGN_10500 0.49 -0.7 843,472 - amiB HEPCGN_10500 0.61 -0.2 843,474 + amiB HEPCGN_10500 0.61 -0.7 843,484 - amiB HEPCGN_10500 0.62 +0.4 843,493 - amiB HEPCGN_10500 0.62 -0.3 843,496 - amiB HEPCGN_10500 0.63 -0.5 843,496 - amiB HEPCGN_10500 0.63 -0.6 843,641 + amiB HEPCGN_10500 0.73 -0.8 843,641 + amiB HEPCGN_10500 0.73 -0.5 843,642 - amiB HEPCGN_10500 0.73 -0.1 843,642 - amiB HEPCGN_10500 0.73 -0.4 843,990 - -1.9 843,990 - -0.7 843,990 - -0.6 843,990 - -0.4 843,990 - -2.0 844,484 - -0.3 844,620 + nnr HEPCGN_10510 0.11 -1.7 844,657 + nnr HEPCGN_10510 0.14 +0.0 844,791 - nnr HEPCGN_10510 0.22 +1.0 844,811 + nnr HEPCGN_10510 0.24 -0.7 844,812 - nnr HEPCGN_10510 0.24 -0.3
Or see this region's nucleotide sequence