Experiment: Experiment:TE, Cucumber 5 Main Stem Xylem (TE-S-C5-SX)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt proB and proA are separated by 15 nucleotides proA and LU632_RS01195 are separated by 83 nucleotides LU632_RS01195 and LU632_RS01200 are separated by 42 nucleotides
LU632_RS01185: proB - glutamate 5-kinase, at 250,192 to 251,295
proB
LU632_RS01190: proA - glutamate-5-semialdehyde dehydrogenase, at 251,311 to 252,564
proA
LU632_RS01195: LU632_RS01195 - LysE family translocator, at 252,648 to 252,767
_RS01195
LU632_RS01200: LU632_RS01200 - HNH endonuclease, at 252,810 to 253,175
_RS01200
Position (kb) 251
252
253 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 250.416 kb on - strand, within proB at 250.472 kb on - strand, within proB at 250.482 kb on + strand, within proB at 250.482 kb on + strand, within proB at 250.484 kb on + strand, within proB at 250.484 kb on + strand, within proB at 250.485 kb on - strand, within proB at 250.485 kb on - strand, within proB at 250.553 kb on + strand, within proB at 250.553 kb on + strand, within proB at 250.553 kb on + strand, within proB at 250.554 kb on - strand, within proB at 250.575 kb on + strand, within proB at 250.575 kb on + strand, within proB at 250.576 kb on - strand, within proB at 250.576 kb on - strand, within proB at 250.809 kb on - strand, within proB at 250.884 kb on + strand, within proB at 250.885 kb on - strand, within proB at 250.885 kb on - strand, within proB at 250.986 kb on + strand, within proB at 251.182 kb on - strand, within proB at 251.221 kb on - strand at 251.245 kb on - strand at 251.281 kb on - strand at 251.529 kb on - strand, within proA at 251.666 kb on - strand, within proA at 251.685 kb on + strand, within proA at 251.870 kb on + strand, within proA at 251.870 kb on + strand, within proA at 251.870 kb on + strand, within proA at 251.870 kb on + strand, within proA at 251.871 kb on - strand, within proA at 251.871 kb on - strand, within proA at 251.874 kb on + strand, within proA at 251.874 kb on + strand, within proA at 251.874 kb on + strand, within proA at 251.875 kb on - strand, within proA at 251.875 kb on - strand, within proA at 252.039 kb on + strand, within proA at 252.040 kb on - strand, within proA at 252.040 kb on - strand, within proA at 252.346 kb on - strand, within proA at 252.523 kb on + strand at 252.523 kb on + strand at 252.523 kb on + strand at 252.559 kb on - strand at 252.572 kb on - strand at 252.576 kb on + strand at 252.577 kb on - strand at 252.577 kb on - strand at 252.591 kb on + strand at 252.591 kb on + strand at 252.592 kb on - strand at 252.701 kb on - strand, within LU632_RS01195 at 252.719 kb on + strand, within LU632_RS01195 at 252.719 kb on + strand, within LU632_RS01195 at 252.720 kb on - strand, within LU632_RS01195 at 252.726 kb on + strand, within LU632_RS01195 at 252.759 kb on - strand at 252.768 kb on + strand at 252.769 kb on - strand at 252.808 kb on - strand at 252.810 kb on - strand at 252.986 kb on - strand, within LU632_RS01200 at 253.018 kb on - strand, within LU632_RS01200 at 253.018 kb on - strand, within LU632_RS01200 at 253.040 kb on + strand, within LU632_RS01200 at 253.077 kb on - strand, within LU632_RS01200 at 253.077 kb on - strand, within LU632_RS01200 at 253.077 kb on - strand, within LU632_RS01200 at 253.085 kb on + strand, within LU632_RS01200 at 253.086 kb on - strand, within LU632_RS01200 at 253.090 kb on - strand, within LU632_RS01200 at 253.248 kb on + strand at 253.248 kb on + strand at 253.249 kb on - strand at 253.252 kb on + strand at 253.263 kb on + strand at 253.267 kb on + strand at 253.267 kb on + strand at 253.267 kb on + strand at 253.268 kb on - strand at 253.268 kb on - strand at 253.268 kb on - strand at 253.268 kb on - strand at 253.268 kb on - strand at 253.345 kb on + strand at 253.360 kb on + strand at 253.517 kb on + strand at 253.518 kb on - strand at 253.518 kb on - strand at 253.520 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Experiment:TE, Cucumber 5 Main Stem Xylem (TE-S-C5-SX) remove 250,416 - proB LU632_RS01185 0.20 -2.1 250,472 - proB LU632_RS01185 0.25 -0.8 250,482 + proB LU632_RS01185 0.26 -0.5 250,482 + proB LU632_RS01185 0.26 +2.3 250,484 + proB LU632_RS01185 0.26 -0.2 250,484 + proB LU632_RS01185 0.26 -1.3 250,485 - proB LU632_RS01185 0.27 -0.2 250,485 - proB LU632_RS01185 0.27 -1.1 250,553 + proB LU632_RS01185 0.33 -0.2 250,553 + proB LU632_RS01185 0.33 -0.5 250,553 + proB LU632_RS01185 0.33 -1.3 250,554 - proB LU632_RS01185 0.33 -3.9 250,575 + proB LU632_RS01185 0.35 -0.4 250,575 + proB LU632_RS01185 0.35 -2.3 250,576 - proB LU632_RS01185 0.35 -1.1 250,576 - proB LU632_RS01185 0.35 -1.3 250,809 - proB LU632_RS01185 0.56 -1.5 250,884 + proB LU632_RS01185 0.63 -0.2 250,885 - proB LU632_RS01185 0.63 +0.4 250,885 - proB LU632_RS01185 0.63 +0.3 250,986 + proB LU632_RS01185 0.72 +0.6 251,182 - proB LU632_RS01185 0.90 -0.5 251,221 - -2.1 251,245 - -1.4 251,281 - -0.7 251,529 - proA LU632_RS01190 0.17 -1.6 251,666 - proA LU632_RS01190 0.28 -2.7 251,685 + proA LU632_RS01190 0.30 -2.7 251,870 + proA LU632_RS01190 0.45 -0.4 251,870 + proA LU632_RS01190 0.45 -1.6 251,870 + proA LU632_RS01190 0.45 -1.1 251,870 + proA LU632_RS01190 0.45 -0.4 251,871 - proA LU632_RS01190 0.45 -1.7 251,871 - proA LU632_RS01190 0.45 +1.1 251,874 + proA LU632_RS01190 0.45 -1.1 251,874 + proA LU632_RS01190 0.45 -1.1 251,874 + proA LU632_RS01190 0.45 -3.5 251,875 - proA LU632_RS01190 0.45 -1.8 251,875 - proA LU632_RS01190 0.45 -0.9 252,039 + proA LU632_RS01190 0.58 -1.5 252,040 - proA LU632_RS01190 0.58 +1.1 252,040 - proA LU632_RS01190 0.58 -4.8 252,346 - proA LU632_RS01190 0.83 -3.0 252,523 + -1.2 252,523 + -3.4 252,523 + -1.7 252,559 - -2.5 252,572 - -0.0 252,576 + -1.6 252,577 - +1.1 252,577 - +0.5 252,591 + +1.0 252,591 + +0.3 252,592 - -1.7 252,701 - LU632_RS01195 0.44 -1.2 252,719 + LU632_RS01195 0.59 -0.0 252,719 + LU632_RS01195 0.59 -1.7 252,720 - LU632_RS01195 0.60 -0.7 252,726 + LU632_RS01195 0.65 -1.4 252,759 - +0.5 252,768 + +0.1 252,769 - +1.1 252,808 - +0.1 252,810 - +0.3 252,986 - LU632_RS01200 0.48 +1.2 253,018 - LU632_RS01200 0.57 +1.3 253,018 - LU632_RS01200 0.57 +0.8 253,040 + LU632_RS01200 0.63 +0.0 253,077 - LU632_RS01200 0.73 -0.8 253,077 - LU632_RS01200 0.73 -2.8 253,077 - LU632_RS01200 0.73 -0.4 253,085 + LU632_RS01200 0.75 -4.4 253,086 - LU632_RS01200 0.75 -0.5 253,090 - LU632_RS01200 0.77 +0.4 253,248 + -0.3 253,248 + +0.4 253,249 - +0.8 253,252 + -0.1 253,263 + +2.8 253,267 + +2.6 253,267 + +2.3 253,267 + -2.2 253,268 - +0.2 253,268 - +2.6 253,268 - +0.6 253,268 - -3.1 253,268 - -0.5 253,345 + +0.2 253,360 + -2.9 253,517 + +2.7 253,518 - +0.5 253,518 - -1.0 253,520 - +0.8
Or see this region's nucleotide sequence