Strain Fitness in Escherichia coli ECOR38 around HEPCGN_24725

Experiment: Bas48

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntdfsB and umuD are separated by 132 nucleotidesumuD and umuC overlap by 1 nucleotidesumuC and dsbB are separated by 45 nucleotidesdsbB and nhaB are separated by 145 nucleotides HEPCGN_24715: dfsB - DUF1706 domain-containing protein, at 3,696,085 to 3,696,603 dfsB HEPCGN_24720: umuD - Protein UmuD, at 3,696,736 to 3,697,155 umuD HEPCGN_24725: umuC - DNA polymerase V catalytic protein, at 3,697,155 to 3,698,423 umuC HEPCGN_24730: dsbB - disulfide bond formation protein DsbB, at 3,698,469 to 3,698,999 dsbB HEPCGN_24735: nhaB - Na(+)/H(+) antiporter NhaB, at 3,699,145 to 3,700,686 nhaB Position (kb) 3697 3698 3699Strain fitness (log2 ratio) -2 -1 0 1at 3696.717 kb on + strandat 3696.718 kb on - strandat 3696.732 kb on + strandat 3696.735 kb on + strandat 3696.736 kb on - strandat 3696.785 kb on + strand, within umuDat 3696.889 kb on + strand, within umuDat 3696.890 kb on - strand, within umuDat 3696.915 kb on + strand, within umuDat 3696.915 kb on + strand, within umuDat 3696.966 kb on + strand, within umuDat 3697.057 kb on - strand, within umuDat 3697.105 kb on - strand, within umuDat 3697.175 kb on - strandat 3697.302 kb on - strand, within umuCat 3697.374 kb on + strand, within umuCat 3697.383 kb on + strand, within umuCat 3697.446 kb on + strand, within umuCat 3697.488 kb on - strand, within umuCat 3697.651 kb on + strand, within umuCat 3697.678 kb on + strand, within umuCat 3697.678 kb on + strand, within umuCat 3697.679 kb on - strand, within umuCat 3697.784 kb on + strand, within umuCat 3697.895 kb on + strand, within umuCat 3697.896 kb on - strand, within umuCat 3697.934 kb on + strand, within umuCat 3697.958 kb on + strand, within umuCat 3697.962 kb on + strand, within umuCat 3698.027 kb on + strand, within umuCat 3698.031 kb on - strand, within umuCat 3698.062 kb on - strand, within umuCat 3698.118 kb on - strand, within umuCat 3698.289 kb on - strand, within umuCat 3698.294 kb on - strand, within umuCat 3698.323 kb on - strandat 3698.387 kb on + strandat 3698.387 kb on + strandat 3698.421 kb on + strandat 3698.428 kb on - strandat 3698.428 kb on - strandat 3698.428 kb on - strandat 3698.542 kb on + strand, within dsbBat 3698.556 kb on - strand, within dsbBat 3698.649 kb on - strand, within dsbBat 3698.762 kb on + strand, within dsbBat 3698.762 kb on + strand, within dsbBat 3698.764 kb on + strand, within dsbBat 3698.764 kb on + strand, within dsbBat 3698.765 kb on - strand, within dsbBat 3698.765 kb on - strand, within dsbBat 3698.765 kb on - strand, within dsbBat 3698.765 kb on - strand, within dsbBat 3698.785 kb on - strand, within dsbBat 3698.788 kb on + strand, within dsbBat 3698.999 kb on + strandat 3699.016 kb on + strandat 3699.017 kb on - strandat 3699.023 kb on - strandat 3699.124 kb on + strandat 3699.126 kb on + strandat 3699.175 kb on - strandat 3699.201 kb on + strandat 3699.232 kb on + strandat 3699.263 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas48
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3,696,717 + +1.7
3,696,718 - +0.7
3,696,732 + -0.1
3,696,735 + -0.2
3,696,736 - +1.4
3,696,785 + umuD HEPCGN_24720 0.12 -0.6
3,696,889 + umuD HEPCGN_24720 0.36 -1.1
3,696,890 - umuD HEPCGN_24720 0.37 +0.6
3,696,915 + umuD HEPCGN_24720 0.43 -0.2
3,696,915 + umuD HEPCGN_24720 0.43 -0.3
3,696,966 + umuD HEPCGN_24720 0.55 -1.3
3,697,057 - umuD HEPCGN_24720 0.76 +0.1
3,697,105 - umuD HEPCGN_24720 0.88 +0.3
3,697,175 - -0.3
3,697,302 - umuC HEPCGN_24725 0.12 -1.4
3,697,374 + umuC HEPCGN_24725 0.17 -0.7
3,697,383 + umuC HEPCGN_24725 0.18 +0.8
3,697,446 + umuC HEPCGN_24725 0.23 +0.8
3,697,488 - umuC HEPCGN_24725 0.26 +1.7
3,697,651 + umuC HEPCGN_24725 0.39 +0.2
3,697,678 + umuC HEPCGN_24725 0.41 -1.1
3,697,678 + umuC HEPCGN_24725 0.41 -0.1
3,697,679 - umuC HEPCGN_24725 0.41 +0.5
3,697,784 + umuC HEPCGN_24725 0.50 -1.4
3,697,895 + umuC HEPCGN_24725 0.58 +0.7
3,697,896 - umuC HEPCGN_24725 0.58 -0.1
3,697,934 + umuC HEPCGN_24725 0.61 -0.2
3,697,958 + umuC HEPCGN_24725 0.63 -0.0
3,697,962 + umuC HEPCGN_24725 0.64 -0.1
3,698,027 + umuC HEPCGN_24725 0.69 -0.9
3,698,031 - umuC HEPCGN_24725 0.69 +0.7
3,698,062 - umuC HEPCGN_24725 0.71 +1.0
3,698,118 - umuC HEPCGN_24725 0.76 +0.9
3,698,289 - umuC HEPCGN_24725 0.89 +0.5
3,698,294 - umuC HEPCGN_24725 0.90 +0.0
3,698,323 - -0.3
3,698,387 + +0.1
3,698,387 + +0.9
3,698,421 + +0.7
3,698,428 - +0.3
3,698,428 - +0.9
3,698,428 - -0.6
3,698,542 + dsbB HEPCGN_24730 0.14 -0.4
3,698,556 - dsbB HEPCGN_24730 0.16 +0.7
3,698,649 - dsbB HEPCGN_24730 0.34 -0.6
3,698,762 + dsbB HEPCGN_24730 0.55 +0.1
3,698,762 + dsbB HEPCGN_24730 0.55 -1.8
3,698,764 + dsbB HEPCGN_24730 0.56 +0.1
3,698,764 + dsbB HEPCGN_24730 0.56 -0.1
3,698,765 - dsbB HEPCGN_24730 0.56 +0.5
3,698,765 - dsbB HEPCGN_24730 0.56 -1.4
3,698,765 - dsbB HEPCGN_24730 0.56 -1.0
3,698,765 - dsbB HEPCGN_24730 0.56 -1.1
3,698,785 - dsbB HEPCGN_24730 0.60 -1.0
3,698,788 + dsbB HEPCGN_24730 0.60 +1.1
3,698,999 + -0.4
3,699,016 + -1.3
3,699,017 - +0.9
3,699,023 - -0.3
3,699,124 + +0.7
3,699,126 + +0.1
3,699,175 - +0.3
3,699,201 + -0.1
3,699,232 + -0.0
3,699,263 - +0.4

Or see this region's nucleotide sequence