Strain Fitness in Escherichia coli ECOR38 around HEPCGN_16985

Experiment: Bas48

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnusA and infB are separated by 24 nucleotidesinfB and rbfA are separated by 163 nucleotidesrbfA and truB overlap by 1 nucleotides HEPCGN_16980: nusA - Transcription termination/antitermination protein NusA, at 2,144,317 to 2,145,804 nusA HEPCGN_16985: infB - Translation initiation factor IF-2, at 2,145,829 to 2,148,501 infB HEPCGN_16995: rbfA - 30S ribosome-binding factor RbfA, at 2,148,665 to 2,149,066 rbfA HEPCGN_17000: truB - tRNA pseudouridine(55) synthase TruB, at 2,149,066 to 2,150,010 truB Position (kb) 2145 2146 2147 2148 2149Strain fitness (log2 ratio) -1 0 1at 2145.472 kb on + strand, within nusAat 2145.777 kb on + strandat 2145.777 kb on + strandat 2145.802 kb on + strandat 2148.489 kb on + strandat 2148.499 kb on + strandat 2149.120 kb on - strandat 2149.171 kb on + strand, within truBat 2149.172 kb on - strand, within truBat 2149.268 kb on - strand, within truBat 2149.456 kb on + strand, within truBat 2149.457 kb on - strand, within truBat 2149.480 kb on + strand, within truB

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas48
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2,145,472 + nusA HEPCGN_16980 0.78 -0.4
2,145,777 + -1.1
2,145,777 + -1.3
2,145,802 + +0.3
2,148,489 + +1.3
2,148,499 + +0.7
2,149,120 - -1.0
2,149,171 + truB HEPCGN_17000 0.11 -1.0
2,149,172 - truB HEPCGN_17000 0.11 +0.0
2,149,268 - truB HEPCGN_17000 0.21 -0.3
2,149,456 + truB HEPCGN_17000 0.41 -1.0
2,149,457 - truB HEPCGN_17000 0.41 -0.4
2,149,480 + truB HEPCGN_17000 0.44 -1.0

Or see this region's nucleotide sequence