Experiment: Bas48
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt degS and degQ are separated by 89 nucleotides degQ and zapG are separated by 153 nucleotides zapG and zapE are separated by 193 nucleotides
HEPCGN_16670: degS - outer membrane-stress sensor serine endopeptidase DegS, at 2,084,939 to 2,086,006
degS
HEPCGN_16675: degQ - serine endoprotease DegQ, at 2,086,096 to 2,087,463
degQ
HEPCGN_16680: zapG - Z-ring associated protein ZapG, at 2,087,617 to 2,088,015
zapG
HEPCGN_16685: zapE - cell division protein ZapE, at 2,088,209 to 2,089,336
zapE
Position (kb)
2086
2087
2088 Strain fitness (log2 ratio)
-1
0
1
2 at 2085.180 kb on - strand, within degS at 2085.227 kb on - strand, within degS at 2085.362 kb on - strand, within degS at 2085.410 kb on - strand, within degS at 2085.590 kb on - strand, within degS at 2085.684 kb on - strand, within degS at 2085.737 kb on - strand, within degS at 2085.759 kb on - strand, within degS at 2085.969 kb on - strand at 2086.112 kb on + strand at 2086.287 kb on + strand, within degQ at 2086.287 kb on + strand, within degQ at 2086.288 kb on - strand, within degQ at 2086.291 kb on - strand, within degQ at 2086.403 kb on - strand, within degQ at 2086.471 kb on - strand, within degQ at 2086.494 kb on + strand, within degQ at 2086.495 kb on - strand, within degQ at 2086.495 kb on - strand, within degQ at 2086.525 kb on - strand, within degQ at 2086.527 kb on + strand, within degQ at 2086.720 kb on - strand, within degQ at 2086.999 kb on - strand, within degQ at 2087.164 kb on - strand, within degQ at 2087.425 kb on + strand at 2087.425 kb on + strand at 2087.426 kb on - strand at 2087.431 kb on + strand at 2087.432 kb on - strand at 2087.478 kb on + strand at 2087.893 kb on - strand, within zapG at 2087.933 kb on - strand, within zapG at 2087.938 kb on - strand, within zapG at 2087.970 kb on + strand, within zapG at 2087.995 kb on + strand at 2088.089 kb on + strand at 2088.099 kb on - strand at 2088.140 kb on + strand at 2088.196 kb on + strand at 2088.202 kb on + strand at 2088.203 kb on - strand at 2088.203 kb on - strand at 2088.315 kb on - strand at 2088.332 kb on - strand, within zapE at 2088.412 kb on + strand, within zapE at 2088.429 kb on + strand, within zapE at 2088.429 kb on + strand, within zapE at 2088.429 kb on + strand, within zapE at 2088.430 kb on - strand, within zapE at 2088.430 kb on - strand, within zapE at 2088.431 kb on + strand, within zapE at 2088.431 kb on + strand, within zapE at 2088.432 kb on - strand, within zapE at 2088.432 kb on - strand, within zapE at 2088.432 kb on - strand, within zapE at 2088.432 kb on - strand, within zapE at 2088.432 kb on - strand, within zapE at 2088.432 kb on - strand, within zapE at 2088.433 kb on + strand, within zapE at 2088.433 kb on + strand, within zapE at 2088.434 kb on - strand, within zapE at 2088.448 kb on - strand, within zapE
Per-strain Table
Position Strand Gene LocusTag Fraction Bas48 remove 2,085,180 - degS HEPCGN_16670 0.23 +2.4 2,085,227 - degS HEPCGN_16670 0.27 +2.4 2,085,362 - degS HEPCGN_16670 0.40 +2.4 2,085,410 - degS HEPCGN_16670 0.44 +0.8 2,085,590 - degS HEPCGN_16670 0.61 +1.4 2,085,684 - degS HEPCGN_16670 0.70 +2.4 2,085,737 - degS HEPCGN_16670 0.75 +2.4 2,085,759 - degS HEPCGN_16670 0.77 +1.4 2,085,969 - +2.4 2,086,112 + +0.4 2,086,287 + degQ HEPCGN_16675 0.14 +0.4 2,086,287 + degQ HEPCGN_16675 0.14 -1.1 2,086,288 - degQ HEPCGN_16675 0.14 -0.1 2,086,291 - degQ HEPCGN_16675 0.14 -1.3 2,086,403 - degQ HEPCGN_16675 0.22 -0.1 2,086,471 - degQ HEPCGN_16675 0.27 +0.5 2,086,494 + degQ HEPCGN_16675 0.29 +0.0 2,086,495 - degQ HEPCGN_16675 0.29 +0.5 2,086,495 - degQ HEPCGN_16675 0.29 +0.3 2,086,525 - degQ HEPCGN_16675 0.31 -0.7 2,086,527 + degQ HEPCGN_16675 0.32 -0.5 2,086,720 - degQ HEPCGN_16675 0.46 -0.9 2,086,999 - degQ HEPCGN_16675 0.66 -0.5 2,087,164 - degQ HEPCGN_16675 0.78 -0.5 2,087,425 + +0.6 2,087,425 + -0.8 2,087,426 - +0.7 2,087,431 + -0.2 2,087,432 - -0.8 2,087,478 + +0.2 2,087,893 - zapG HEPCGN_16680 0.69 +0.4 2,087,933 - zapG HEPCGN_16680 0.79 +1.4 2,087,938 - zapG HEPCGN_16680 0.80 +2.4 2,087,970 + zapG HEPCGN_16680 0.88 +0.8 2,087,995 + +1.4 2,088,089 + +1.4 2,088,099 - -0.6 2,088,140 + +1.4 2,088,196 + +0.4 2,088,202 + -0.4 2,088,203 - +1.1 2,088,203 - -0.9 2,088,315 - -0.4 2,088,332 - zapE HEPCGN_16685 0.11 +0.0 2,088,412 + zapE HEPCGN_16685 0.18 +1.1 2,088,429 + zapE HEPCGN_16685 0.20 -1.2 2,088,429 + zapE HEPCGN_16685 0.20 -0.1 2,088,429 + zapE HEPCGN_16685 0.20 +0.0 2,088,430 - zapE HEPCGN_16685 0.20 +0.0 2,088,430 - zapE HEPCGN_16685 0.20 -1.4 2,088,431 + zapE HEPCGN_16685 0.20 -0.7 2,088,431 + zapE HEPCGN_16685 0.20 -0.2 2,088,432 - zapE HEPCGN_16685 0.20 -0.6 2,088,432 - zapE HEPCGN_16685 0.20 +0.3 2,088,432 - zapE HEPCGN_16685 0.20 -1.6 2,088,432 - zapE HEPCGN_16685 0.20 -0.3 2,088,432 - zapE HEPCGN_16685 0.20 -0.9 2,088,432 - zapE HEPCGN_16685 0.20 -0.1 2,088,433 + zapE HEPCGN_16685 0.20 -0.0 2,088,433 + zapE HEPCGN_16685 0.20 +0.2 2,088,434 - zapE HEPCGN_16685 0.20 +0.6 2,088,448 - zapE HEPCGN_16685 0.21 +1.4
Or see this region's nucleotide sequence