Strain Fitness in Escherichia coli ECOR38 around HEPCGN_13170

Experiment: Bas48

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcyaA and hemC are separated by 365 nucleotideshemC and hemD overlap by 4 nucleotideshemD and hemX are separated by 21 nucleotides HEPCGN_13165: cyaA - class I adenylate cyclase, at 1,377,009 to 1,379,555 cyaA HEPCGN_13170: hemC - hydroxymethylbilane synthase, at 1,379,921 to 1,380,883 hemC HEPCGN_13175: hemD - uroporphyrinogen-III synthase, at 1,380,880 to 1,381,620 hemD HEPCGN_13180: hemX - uroporphyrinogen-III C-methyltransferase, at 1,381,642 to 1,382,859 hemX Position (kb) 1379 1380 1381Strain fitness (log2 ratio) -2 -1 0 1at 1379.546 kb on - strandat 1379.546 kb on - strandat 1379.561 kb on - strandat 1379.649 kb on - strandat 1379.757 kb on - strandat 1379.760 kb on + strandat 1379.768 kb on + strandat 1379.768 kb on + strandat 1379.769 kb on - strandat 1379.799 kb on + strandat 1379.846 kb on + strandat 1379.866 kb on - strandat 1379.906 kb on + strandat 1381.613 kb on + strandat 1381.614 kb on - strandat 1381.614 kb on - strandat 1381.618 kb on + strandat 1381.618 kb on + strandat 1381.640 kb on + strandat 1381.641 kb on - strandat 1381.776 kb on + strand, within hemXat 1381.804 kb on + strand, within hemXat 1381.804 kb on + strand, within hemX

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas48
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1,379,546 - -0.3
1,379,546 - +0.2
1,379,561 - -0.3
1,379,649 - +0.2
1,379,757 - +1.3
1,379,760 + -0.4
1,379,768 + +0.2
1,379,768 + -0.3
1,379,769 - -1.4
1,379,799 + -0.3
1,379,846 + +0.7
1,379,866 - -0.2
1,379,906 + +0.9
1,381,613 + -0.1
1,381,614 - -0.8
1,381,614 - -1.6
1,381,618 + +0.5
1,381,618 + -1.9
1,381,640 + -0.4
1,381,641 - +0.5
1,381,776 + hemX HEPCGN_13180 0.11 -0.8
1,381,804 + hemX HEPCGN_13180 0.13 -0.7
1,381,804 + hemX HEPCGN_13180 0.13 -0.5

Or see this region's nucleotide sequence