Experiment: Bas48
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt amiB and tsaE are separated by 18 nucleotides tsaE and nnr overlap by 29 nucleotides nnr and queG are separated by 13 nucleotides
HEPCGN_10500: amiB - N-acetylmuramoyl-L-alanine amidase AmiB, at 842,659 to 843,996
amiB
HEPCGN_10505: tsaE - tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE, at 844,015 to 844,476
tsaE
HEPCGN_10510: nnr - bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase, at 844,448 to 845,980
nnr
HEPCGN_10515: queG - tRNA epoxyqueuosine(34) reductase QueG, at 845,994 to 847,133
queG
Position (kb) 844
845
846 Strain fitness (log2 ratio) -2
-1
0
1
2 at 843.472 kb on - strand, within amiB at 843.474 kb on + strand, within amiB at 843.484 kb on - strand, within amiB at 843.493 kb on - strand, within amiB at 843.496 kb on - strand, within amiB at 843.496 kb on - strand, within amiB at 843.641 kb on + strand, within amiB at 843.641 kb on + strand, within amiB at 843.642 kb on - strand, within amiB at 843.642 kb on - strand, within amiB at 843.990 kb on - strand at 843.990 kb on - strand at 843.990 kb on - strand at 843.990 kb on - strand at 843.990 kb on - strand at 844.484 kb on - strand at 844.620 kb on + strand, within nnr at 844.657 kb on + strand, within nnr at 844.791 kb on - strand, within nnr at 844.811 kb on + strand, within nnr at 844.812 kb on - strand, within nnr at 845.188 kb on + strand, within nnr at 845.416 kb on + strand, within nnr at 845.459 kb on - strand, within nnr at 845.522 kb on - strand, within nnr at 845.525 kb on - strand, within nnr at 845.545 kb on + strand, within nnr at 845.570 kb on + strand, within nnr at 845.734 kb on + strand, within nnr at 845.767 kb on - strand, within nnr at 845.767 kb on - strand, within nnr at 845.785 kb on + strand, within nnr at 845.881 kb on - strand at 845.881 kb on - strand at 845.959 kb on + strand at 845.960 kb on - strand at 845.983 kb on + strand at 845.983 kb on + strand at 845.983 kb on + strand at 845.984 kb on - strand at 845.984 kb on - strand at 846.022 kb on + strand at 846.071 kb on - strand at 846.071 kb on - strand at 846.074 kb on + strand at 846.074 kb on + strand at 846.074 kb on + strand at 846.136 kb on - strand, within queG at 846.197 kb on - strand, within queG at 846.237 kb on + strand, within queG at 846.238 kb on - strand, within queG at 846.291 kb on + strand, within queG at 846.291 kb on + strand, within queG at 846.322 kb on - strand, within queG at 846.420 kb on - strand, within queG at 846.467 kb on + strand, within queG at 846.591 kb on - strand, within queG at 846.628 kb on - strand, within queG at 846.630 kb on - strand, within queG at 846.630 kb on - strand, within queG at 846.630 kb on - strand, within queG at 846.630 kb on - strand, within queG at 846.658 kb on - strand, within queG at 846.723 kb on + strand, within queG at 846.747 kb on - strand, within queG at 846.894 kb on - strand, within queG at 846.895 kb on + strand, within queG at 846.895 kb on + strand, within queG at 846.928 kb on + strand, within queG
Per-strain Table
Position Strand Gene LocusTag Fraction Bas48 remove 843,472 - amiB HEPCGN_10500 0.61 -0.2 843,474 + amiB HEPCGN_10500 0.61 +0.5 843,484 - amiB HEPCGN_10500 0.62 +0.4 843,493 - amiB HEPCGN_10500 0.62 +0.3 843,496 - amiB HEPCGN_10500 0.63 -0.5 843,496 - amiB HEPCGN_10500 0.63 -1.5 843,641 + amiB HEPCGN_10500 0.73 +0.2 843,641 + amiB HEPCGN_10500 0.73 +1.1 843,642 - amiB HEPCGN_10500 0.73 +0.2 843,642 - amiB HEPCGN_10500 0.73 +0.3 843,990 - -0.4 843,990 - -0.1 843,990 - +1.1 843,990 - -0.5 843,990 - -0.3 844,484 - -0.8 844,620 + nnr HEPCGN_10510 0.11 -1.8 844,657 + nnr HEPCGN_10510 0.14 +0.4 844,791 - nnr HEPCGN_10510 0.22 +2.0 844,811 + nnr HEPCGN_10510 0.24 -0.5 844,812 - nnr HEPCGN_10510 0.24 -1.3 845,188 + nnr HEPCGN_10510 0.48 +0.6 845,416 + nnr HEPCGN_10510 0.63 +0.3 845,459 - nnr HEPCGN_10510 0.66 +0.7 845,522 - nnr HEPCGN_10510 0.70 -0.1 845,525 - nnr HEPCGN_10510 0.70 -0.4 845,545 + nnr HEPCGN_10510 0.72 -0.5 845,570 + nnr HEPCGN_10510 0.73 -0.2 845,734 + nnr HEPCGN_10510 0.84 +1.4 845,767 - nnr HEPCGN_10510 0.86 +0.7 845,767 - nnr HEPCGN_10510 0.86 -1.3 845,785 + nnr HEPCGN_10510 0.87 -0.4 845,881 - +0.4 845,881 - +0.2 845,959 + -0.3 845,960 - +0.6 845,983 + -0.4 845,983 + +0.5 845,983 + -0.1 845,984 - -0.6 845,984 - +0.6 846,022 + +1.4 846,071 - -0.5 846,071 - -1.9 846,074 + +1.1 846,074 + -0.5 846,074 + -0.8 846,136 - queG HEPCGN_10515 0.12 -0.4 846,197 - queG HEPCGN_10515 0.18 +1.4 846,237 + queG HEPCGN_10515 0.21 -0.8 846,238 - queG HEPCGN_10515 0.21 -0.0 846,291 + queG HEPCGN_10515 0.26 -0.1 846,291 + queG HEPCGN_10515 0.26 -0.2 846,322 - queG HEPCGN_10515 0.29 -0.4 846,420 - queG HEPCGN_10515 0.37 +2.3 846,467 + queG HEPCGN_10515 0.41 -0.1 846,591 - queG HEPCGN_10515 0.52 -0.8 846,628 - queG HEPCGN_10515 0.56 -0.0 846,630 - queG HEPCGN_10515 0.56 +0.2 846,630 - queG HEPCGN_10515 0.56 -0.5 846,630 - queG HEPCGN_10515 0.56 -0.4 846,630 - queG HEPCGN_10515 0.56 +0.1 846,658 - queG HEPCGN_10515 0.58 -0.6 846,723 + queG HEPCGN_10515 0.64 -0.0 846,747 - queG HEPCGN_10515 0.66 +2.3 846,894 - queG HEPCGN_10515 0.79 -0.8 846,895 + queG HEPCGN_10515 0.79 -1.8 846,895 + queG HEPCGN_10515 0.79 +1.6 846,928 + queG HEPCGN_10515 0.82 +0.2
Or see this region's nucleotide sequence