Experiment: Experiment:TE, Cucumber 5 Main Stem (TE-S-C5-MS)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mnmG and mioC are separated by 436 nucleotides mioC and LU632_RS24825 are separated by 328 nucleotides
LU632_RS24815: mnmG - tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG, at 4,726,946 to 4,728,835
mnmG
LU632_RS24820: mioC - FMN-binding protein MioC, at 4,729,272 to 4,729,715
mioC
LU632_RS24825: LU632_RS24825 - IS91 family transposase, at 4,730,044 to 4,731,267
_RS24825
Position (kb)
4729
4730 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4728.890 kb on - strand at 4728.896 kb on - strand at 4728.905 kb on + strand at 4728.931 kb on - strand at 4728.931 kb on - strand at 4729.209 kb on + strand at 4729.215 kb on - strand at 4729.463 kb on - strand, within mioC at 4729.467 kb on + strand, within mioC at 4729.467 kb on + strand, within mioC at 4729.472 kb on + strand, within mioC at 4729.473 kb on - strand, within mioC at 4729.473 kb on - strand, within mioC at 4729.477 kb on - strand, within mioC at 4729.560 kb on + strand, within mioC at 4729.560 kb on + strand, within mioC at 4729.561 kb on - strand, within mioC at 4729.561 kb on - strand, within mioC at 4729.608 kb on + strand, within mioC at 4729.612 kb on - strand, within mioC at 4729.612 kb on - strand, within mioC at 4729.612 kb on - strand, within mioC at 4729.666 kb on + strand, within mioC at 4729.666 kb on + strand, within mioC at 4729.680 kb on + strand at 4729.680 kb on + strand at 4729.704 kb on + strand at 4729.705 kb on - strand at 4729.738 kb on + strand at 4729.742 kb on + strand at 4729.747 kb on + strand at 4729.747 kb on + strand at 4729.759 kb on - strand at 4729.770 kb on + strand at 4729.771 kb on - strand at 4729.780 kb on + strand at 4729.810 kb on - strand at 4729.810 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Experiment:TE, Cucumber 5 Main Stem (TE-S-C5-MS) remove 4,728,890 - +1.2 4,728,896 - -0.3 4,728,905 + +0.9 4,728,931 - +1.1 4,728,931 - -2.9 4,729,209 + -0.3 4,729,215 - -0.6 4,729,463 - mioC LU632_RS24820 0.43 -1.4 4,729,467 + mioC LU632_RS24820 0.44 -0.1 4,729,467 + mioC LU632_RS24820 0.44 -0.2 4,729,472 + mioC LU632_RS24820 0.45 +0.6 4,729,473 - mioC LU632_RS24820 0.45 +0.4 4,729,473 - mioC LU632_RS24820 0.45 +0.9 4,729,477 - mioC LU632_RS24820 0.46 +0.8 4,729,560 + mioC LU632_RS24820 0.65 +0.2 4,729,560 + mioC LU632_RS24820 0.65 +1.7 4,729,561 - mioC LU632_RS24820 0.65 +0.0 4,729,561 - mioC LU632_RS24820 0.65 -2.5 4,729,608 + mioC LU632_RS24820 0.76 +0.2 4,729,612 - mioC LU632_RS24820 0.77 -0.3 4,729,612 - mioC LU632_RS24820 0.77 +0.2 4,729,612 - mioC LU632_RS24820 0.77 -1.2 4,729,666 + mioC LU632_RS24820 0.89 +2.3 4,729,666 + mioC LU632_RS24820 0.89 -0.2 4,729,680 + +1.3 4,729,680 + -0.7 4,729,704 + +0.0 4,729,705 - +0.1 4,729,738 + -1.1 4,729,742 + +1.1 4,729,747 + -0.1 4,729,747 + +3.1 4,729,759 - +0.6 4,729,770 + +0.9 4,729,771 - -1.5 4,729,780 + -0.0 4,729,810 - +2.7 4,729,810 - -2.0
Or see this region's nucleotide sequence