Experiment: Experiment:TE, Cucumber 5 Main Stem (TE-S-C5-MS)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lpoB and LU632_RS14770 are separated by 41 nucleotides LU632_RS14770 and hinT are separated by 49 nucleotides hinT and ptsG are separated by 190 nucleotides
LU632_RS14760: thiK - thiamine kinase, at 2,806,523 to 2,807,392
thiK
LU632_RS14765: lpoB - penicillin-binding protein activator LpoB, at 2,807,352 to 2,807,918
lpoB
LU632_RS14770: LU632_RS14770 - YcfL family protein, at 2,807,960 to 2,808,307
_RS14770
LU632_RS14775: hinT - purine nucleoside phosphoramidase, at 2,808,357 to 2,808,707
hinT
LU632_RS14780: ptsG - PTS glucose transporter subunit IIBC, at 2,808,898 to 2,810,331
ptsG
Position (kb)
2808
2809 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2807.453 kb on + strand, within lpoB at 2807.453 kb on + strand, within lpoB at 2807.457 kb on - strand, within lpoB at 2807.457 kb on - strand, within lpoB at 2807.458 kb on + strand, within lpoB at 2807.467 kb on + strand, within lpoB at 2807.655 kb on - strand, within lpoB at 2807.655 kb on - strand, within lpoB at 2807.784 kb on + strand, within lpoB at 2807.934 kb on - strand at 2807.934 kb on - strand at 2807.961 kb on + strand at 2807.967 kb on + strand at 2807.986 kb on + strand at 2807.986 kb on + strand at 2807.987 kb on - strand at 2807.987 kb on - strand at 2808.096 kb on + strand, within LU632_RS14770 at 2808.097 kb on - strand, within LU632_RS14770 at 2808.112 kb on - strand, within LU632_RS14770 at 2808.116 kb on - strand, within LU632_RS14770 at 2808.208 kb on + strand, within LU632_RS14770 at 2808.209 kb on - strand, within LU632_RS14770 at 2808.209 kb on - strand, within LU632_RS14770 at 2808.209 kb on - strand, within LU632_RS14770 at 2808.209 kb on - strand, within LU632_RS14770 at 2808.263 kb on - strand, within LU632_RS14770 at 2808.320 kb on + strand at 2808.351 kb on + strand at 2808.449 kb on + strand, within hinT at 2808.573 kb on - strand, within hinT at 2808.653 kb on + strand, within hinT at 2808.654 kb on - strand, within hinT at 2808.678 kb on - strand at 2808.683 kb on + strand at 2808.831 kb on + strand at 2808.831 kb on + strand at 2808.832 kb on - strand at 2808.900 kb on - strand at 2808.914 kb on + strand at 2808.914 kb on + strand at 2808.914 kb on + strand at 2808.915 kb on - strand at 2808.924 kb on + strand at 2809.098 kb on + strand, within ptsG at 2809.189 kb on - strand, within ptsG at 2809.207 kb on + strand, within ptsG at 2809.208 kb on - strand, within ptsG at 2809.226 kb on + strand, within ptsG at 2809.292 kb on - strand, within ptsG at 2809.292 kb on - strand, within ptsG at 2809.354 kb on - strand, within ptsG at 2809.419 kb on + strand, within ptsG at 2809.420 kb on - strand, within ptsG at 2809.529 kb on - strand, within ptsG at 2809.529 kb on - strand, within ptsG at 2809.530 kb on + strand, within ptsG at 2809.530 kb on + strand, within ptsG at 2809.530 kb on + strand, within ptsG at 2809.531 kb on - strand, within ptsG at 2809.531 kb on - strand, within ptsG at 2809.556 kb on + strand, within ptsG at 2809.588 kb on - strand, within ptsG at 2809.613 kb on + strand, within ptsG at 2809.613 kb on + strand, within ptsG at 2809.656 kb on + strand, within ptsG at 2809.695 kb on + strand, within ptsG
Per-strain Table
Position Strand Gene LocusTag Fraction Experiment:TE, Cucumber 5 Main Stem (TE-S-C5-MS) remove 2,807,453 + lpoB LU632_RS14765 0.18 -0.2 2,807,453 + lpoB LU632_RS14765 0.18 -1.6 2,807,457 - lpoB LU632_RS14765 0.19 -2.7 2,807,457 - lpoB LU632_RS14765 0.19 +1.1 2,807,458 + lpoB LU632_RS14765 0.19 +1.0 2,807,467 + lpoB LU632_RS14765 0.20 -1.0 2,807,655 - lpoB LU632_RS14765 0.53 +0.2 2,807,655 - lpoB LU632_RS14765 0.53 -1.2 2,807,784 + lpoB LU632_RS14765 0.76 +0.6 2,807,934 - -0.4 2,807,934 - +0.5 2,807,961 + -0.8 2,807,967 + -0.2 2,807,986 + -0.7 2,807,986 + -0.5 2,807,987 - -0.7 2,807,987 - +0.3 2,808,096 + LU632_RS14770 0.39 +3.9 2,808,097 - LU632_RS14770 0.39 +0.1 2,808,112 - LU632_RS14770 0.44 -0.6 2,808,116 - LU632_RS14770 0.45 -0.4 2,808,208 + LU632_RS14770 0.71 -1.3 2,808,209 - LU632_RS14770 0.72 -0.8 2,808,209 - LU632_RS14770 0.72 -0.4 2,808,209 - LU632_RS14770 0.72 +2.8 2,808,209 - LU632_RS14770 0.72 +1.8 2,808,263 - LU632_RS14770 0.87 +1.9 2,808,320 + +1.4 2,808,351 + -0.0 2,808,449 + hinT LU632_RS14775 0.26 -0.4 2,808,573 - hinT LU632_RS14775 0.62 +0.2 2,808,653 + hinT LU632_RS14775 0.84 +0.7 2,808,654 - hinT LU632_RS14775 0.85 -0.6 2,808,678 - -0.9 2,808,683 + +0.6 2,808,831 + +3.8 2,808,831 + +0.4 2,808,832 - -2.1 2,808,900 - +0.0 2,808,914 + -0.3 2,808,914 + +0.4 2,808,914 + +0.1 2,808,915 - -3.4 2,808,924 + -0.2 2,809,098 + ptsG LU632_RS14780 0.14 -0.8 2,809,189 - ptsG LU632_RS14780 0.20 +0.4 2,809,207 + ptsG LU632_RS14780 0.22 -1.7 2,809,208 - ptsG LU632_RS14780 0.22 +0.3 2,809,226 + ptsG LU632_RS14780 0.23 +0.1 2,809,292 - ptsG LU632_RS14780 0.27 -0.5 2,809,292 - ptsG LU632_RS14780 0.27 -3.4 2,809,354 - ptsG LU632_RS14780 0.32 +1.0 2,809,419 + ptsG LU632_RS14780 0.36 +1.8 2,809,420 - ptsG LU632_RS14780 0.36 -2.0 2,809,529 - ptsG LU632_RS14780 0.44 +2.1 2,809,529 - ptsG LU632_RS14780 0.44 -0.8 2,809,530 + ptsG LU632_RS14780 0.44 +2.8 2,809,530 + ptsG LU632_RS14780 0.44 -1.2 2,809,530 + ptsG LU632_RS14780 0.44 +0.1 2,809,531 - ptsG LU632_RS14780 0.44 -2.0 2,809,531 - ptsG LU632_RS14780 0.44 -0.6 2,809,556 + ptsG LU632_RS14780 0.46 -0.9 2,809,588 - ptsG LU632_RS14780 0.48 -1.2 2,809,613 + ptsG LU632_RS14780 0.50 +1.3 2,809,613 + ptsG LU632_RS14780 0.50 -0.7 2,809,656 + ptsG LU632_RS14780 0.53 +2.1 2,809,695 + ptsG LU632_RS14780 0.56 -0.1
Or see this region's nucleotide sequence