Experiment: Experiment:TE, Cucumber 5 Main Stem (TE-S-C5-MS)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt galS and mglB are separated by 337 nucleotides mglB and mglA are separated by 90 nucleotides
LU632_RS08515: galS - HTH-type transcriptional regulator GalS, at 1,597,768 to 1,598,766
galS
LU632_RS08520: mglB - galactose/glucose ABC transporter substrate-binding protein MglB, at 1,599,104 to 1,600,099
mglB
LU632_RS08525: mglA - galactose/methyl galactoside ABC transporter ATP-binding protein MglA, at 1,600,190 to 1,601,710
mglA
Position (kb)
1599
1600
1601 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1598.169 kb on - strand, within galS at 1598.218 kb on + strand, within galS at 1598.385 kb on + strand, within galS at 1598.386 kb on - strand, within galS at 1598.386 kb on - strand, within galS at 1598.386 kb on - strand, within galS at 1598.429 kb on + strand, within galS at 1598.430 kb on - strand, within galS at 1598.498 kb on - strand, within galS at 1598.643 kb on + strand, within galS at 1598.785 kb on - strand at 1598.977 kb on + strand at 1599.076 kb on + strand at 1599.084 kb on + strand at 1599.100 kb on + strand at 1599.100 kb on + strand at 1599.110 kb on - strand at 1599.157 kb on + strand at 1599.200 kb on + strand at 1599.202 kb on + strand at 1599.203 kb on - strand at 1599.203 kb on - strand at 1599.327 kb on + strand, within mglB at 1599.328 kb on - strand, within mglB at 1599.425 kb on - strand, within mglB at 1599.477 kb on - strand, within mglB at 1599.488 kb on + strand, within mglB at 1599.491 kb on + strand, within mglB at 1599.492 kb on - strand, within mglB at 1599.492 kb on - strand, within mglB at 1599.492 kb on - strand, within mglB at 1599.599 kb on + strand, within mglB at 1599.599 kb on + strand, within mglB at 1599.653 kb on + strand, within mglB at 1599.654 kb on - strand, within mglB at 1599.654 kb on - strand, within mglB at 1599.654 kb on - strand, within mglB at 1599.848 kb on - strand, within mglB at 1599.848 kb on - strand, within mglB at 1599.867 kb on + strand, within mglB at 1599.929 kb on - strand, within mglB at 1600.022 kb on + strand at 1600.022 kb on + strand at 1600.022 kb on + strand at 1600.041 kb on + strand at 1600.042 kb on - strand at 1600.044 kb on + strand at 1600.055 kb on + strand at 1600.098 kb on - strand at 1600.189 kb on + strand at 1600.372 kb on + strand, within mglA at 1600.372 kb on + strand, within mglA at 1600.375 kb on - strand, within mglA at 1600.375 kb on - strand, within mglA at 1600.376 kb on + strand, within mglA at 1600.377 kb on - strand, within mglA at 1600.479 kb on + strand, within mglA at 1600.479 kb on + strand, within mglA at 1600.756 kb on + strand, within mglA at 1600.759 kb on + strand, within mglA at 1600.800 kb on - strand, within mglA at 1600.906 kb on + strand, within mglA at 1600.992 kb on + strand, within mglA at 1600.993 kb on - strand, within mglA at 1600.993 kb on - strand, within mglA at 1601.024 kb on - strand, within mglA at 1601.032 kb on + strand, within mglA at 1601.032 kb on + strand, within mglA at 1601.033 kb on - strand, within mglA at 1601.033 kb on - strand, within mglA at 1601.034 kb on + strand, within mglA at 1601.035 kb on - strand, within mglA
Per-strain Table
Position Strand Gene LocusTag Fraction Experiment:TE, Cucumber 5 Main Stem (TE-S-C5-MS) remove 1,598,169 - galS LU632_RS08515 0.40 +1.0 1,598,218 + galS LU632_RS08515 0.45 +0.3 1,598,385 + galS LU632_RS08515 0.62 +1.5 1,598,386 - galS LU632_RS08515 0.62 +0.3 1,598,386 - galS LU632_RS08515 0.62 +1.0 1,598,386 - galS LU632_RS08515 0.62 +1.8 1,598,429 + galS LU632_RS08515 0.66 +1.2 1,598,430 - galS LU632_RS08515 0.66 +2.4 1,598,498 - galS LU632_RS08515 0.73 -0.2 1,598,643 + galS LU632_RS08515 0.88 +1.4 1,598,785 - -0.4 1,598,977 + -1.5 1,599,076 + +0.8 1,599,084 + +0.8 1,599,100 + +1.2 1,599,100 + +1.0 1,599,110 - -1.5 1,599,157 + -1.6 1,599,200 + -0.8 1,599,202 + +1.1 1,599,203 - +3.8 1,599,203 - -1.3 1,599,327 + mglB LU632_RS08520 0.22 -1.2 1,599,328 - mglB LU632_RS08520 0.22 +1.3 1,599,425 - mglB LU632_RS08520 0.32 +1.5 1,599,477 - mglB LU632_RS08520 0.37 +0.6 1,599,488 + mglB LU632_RS08520 0.39 -0.4 1,599,491 + mglB LU632_RS08520 0.39 +0.8 1,599,492 - mglB LU632_RS08520 0.39 -1.8 1,599,492 - mglB LU632_RS08520 0.39 +0.4 1,599,492 - mglB LU632_RS08520 0.39 +1.4 1,599,599 + mglB LU632_RS08520 0.50 +0.9 1,599,599 + mglB LU632_RS08520 0.50 +1.2 1,599,653 + mglB LU632_RS08520 0.55 -2.4 1,599,654 - mglB LU632_RS08520 0.55 +0.7 1,599,654 - mglB LU632_RS08520 0.55 +0.8 1,599,654 - mglB LU632_RS08520 0.55 -1.3 1,599,848 - mglB LU632_RS08520 0.75 -1.2 1,599,848 - mglB LU632_RS08520 0.75 +0.6 1,599,867 + mglB LU632_RS08520 0.77 +0.3 1,599,929 - mglB LU632_RS08520 0.83 -0.6 1,600,022 + +4.6 1,600,022 + -0.4 1,600,022 + +0.3 1,600,041 + +1.4 1,600,042 - -0.8 1,600,044 + +0.8 1,600,055 + -1.2 1,600,098 - -1.2 1,600,189 + +0.8 1,600,372 + mglA LU632_RS08525 0.12 -0.9 1,600,372 + mglA LU632_RS08525 0.12 -0.2 1,600,375 - mglA LU632_RS08525 0.12 +1.0 1,600,375 - mglA LU632_RS08525 0.12 -1.4 1,600,376 + mglA LU632_RS08525 0.12 -0.1 1,600,377 - mglA LU632_RS08525 0.12 +0.4 1,600,479 + mglA LU632_RS08525 0.19 +2.1 1,600,479 + mglA LU632_RS08525 0.19 +0.6 1,600,756 + mglA LU632_RS08525 0.37 -2.1 1,600,759 + mglA LU632_RS08525 0.37 -2.4 1,600,800 - mglA LU632_RS08525 0.40 +1.3 1,600,906 + mglA LU632_RS08525 0.47 +0.4 1,600,992 + mglA LU632_RS08525 0.53 -0.0 1,600,993 - mglA LU632_RS08525 0.53 -1.7 1,600,993 - mglA LU632_RS08525 0.53 +0.2 1,601,024 - mglA LU632_RS08525 0.55 -0.2 1,601,032 + mglA LU632_RS08525 0.55 -3.4 1,601,032 + mglA LU632_RS08525 0.55 +0.5 1,601,033 - mglA LU632_RS08525 0.55 -0.1 1,601,033 - mglA LU632_RS08525 0.55 -0.3 1,601,034 + mglA LU632_RS08525 0.55 -0.1 1,601,035 - mglA LU632_RS08525 0.56 -0.5
Or see this region's nucleotide sequence