Experiment: Experiment:TE, Cucumber 5 Main Stem (TE-S-C5-MS)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mutT and yacG are separated by 27 nucleotides yacG and zapD are separated by 11 nucleotides zapD and coaE are separated by 4 nucleotides coaE and LU632_RS03020 are separated by 245 nucleotides
LU632_RS03000: mutT - 8-oxo-dGTP diphosphatase MutT, at 614,541 to 614,936
mutT
LU632_RS03005: yacG - DNA gyrase inhibitor YacG, at 614,964 to 615,173
yacG
LU632_RS03010: zapD - cell division protein ZapD, at 615,185 to 615,928
zapD
LU632_RS03015: coaE - dephospho-CoA kinase, at 615,933 to 616,544
coaE
LU632_RS03020: LU632_RS03020 - GMP reductase, at 616,790 to 617,830
_RS03020
Position (kb) 615
616
617 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 614.934 kb on + strand at 614.935 kb on - strand at 614.935 kb on - strand at 614.965 kb on + strand at 614.982 kb on - strand at 615.000 kb on - strand, within yacG at 615.178 kb on + strand at 615.186 kb on + strand at 615.265 kb on - strand, within zapD at 615.326 kb on + strand, within zapD at 615.381 kb on - strand, within zapD at 615.508 kb on + strand, within zapD at 615.596 kb on + strand, within zapD at 615.656 kb on + strand, within zapD at 615.752 kb on + strand, within zapD at 615.753 kb on - strand, within zapD at 615.753 kb on - strand, within zapD at 615.753 kb on - strand, within zapD at 615.753 kb on - strand, within zapD at 615.845 kb on + strand, within zapD at 616.552 kb on + strand at 616.555 kb on - strand at 616.560 kb on - strand at 616.629 kb on + strand at 616.629 kb on + strand at 616.630 kb on - strand at 616.640 kb on + strand at 616.679 kb on - strand at 616.720 kb on - strand at 616.720 kb on - strand at 616.752 kb on - strand at 616.755 kb on - strand at 616.755 kb on - strand at 616.760 kb on - strand at 616.809 kb on + strand at 616.830 kb on + strand at 616.830 kb on + strand at 616.830 kb on + strand at 616.830 kb on + strand at 616.830 kb on + strand at 616.830 kb on + strand at 616.831 kb on - strand at 616.936 kb on + strand, within LU632_RS03020 at 616.948 kb on + strand, within LU632_RS03020 at 616.969 kb on + strand, within LU632_RS03020 at 616.970 kb on - strand, within LU632_RS03020 at 616.970 kb on - strand, within LU632_RS03020 at 616.970 kb on - strand, within LU632_RS03020 at 616.970 kb on - strand, within LU632_RS03020 at 617.025 kb on - strand, within LU632_RS03020 at 617.025 kb on - strand, within LU632_RS03020 at 617.025 kb on - strand, within LU632_RS03020 at 617.073 kb on + strand, within LU632_RS03020 at 617.095 kb on + strand, within LU632_RS03020 at 617.123 kb on - strand, within LU632_RS03020 at 617.128 kb on - strand, within LU632_RS03020 at 617.178 kb on + strand, within LU632_RS03020 at 617.179 kb on - strand, within LU632_RS03020 at 617.260 kb on + strand, within LU632_RS03020 at 617.311 kb on + strand, within LU632_RS03020 at 617.312 kb on - strand, within LU632_RS03020 at 617.370 kb on + strand, within LU632_RS03020 at 617.397 kb on + strand, within LU632_RS03020 at 617.471 kb on - strand, within LU632_RS03020 at 617.472 kb on + strand, within LU632_RS03020 at 617.472 kb on + strand, within LU632_RS03020 at 617.472 kb on + strand, within LU632_RS03020 at 617.472 kb on + strand, within LU632_RS03020 at 617.473 kb on - strand, within LU632_RS03020 at 617.473 kb on - strand, within LU632_RS03020 at 617.473 kb on - strand, within LU632_RS03020 at 617.518 kb on + strand, within LU632_RS03020 at 617.519 kb on - strand, within LU632_RS03020
Per-strain Table
Position Strand Gene LocusTag Fraction Experiment:TE, Cucumber 5 Main Stem (TE-S-C5-MS) remove 614,934 + -1.3 614,935 - +1.8 614,935 - -1.0 614,965 + +0.4 614,982 - -1.1 615,000 - yacG LU632_RS03005 0.17 +0.3 615,178 + -1.4 615,186 + -0.5 615,265 - zapD LU632_RS03010 0.11 -0.5 615,326 + zapD LU632_RS03010 0.19 -3.1 615,381 - zapD LU632_RS03010 0.26 -1.2 615,508 + zapD LU632_RS03010 0.43 -0.1 615,596 + zapD LU632_RS03010 0.55 +0.2 615,656 + zapD LU632_RS03010 0.63 -0.3 615,752 + zapD LU632_RS03010 0.76 +1.1 615,753 - zapD LU632_RS03010 0.76 +0.2 615,753 - zapD LU632_RS03010 0.76 -0.4 615,753 - zapD LU632_RS03010 0.76 +0.2 615,753 - zapD LU632_RS03010 0.76 +0.6 615,845 + zapD LU632_RS03010 0.89 -0.2 616,552 + +0.1 616,555 - +0.8 616,560 - +0.3 616,629 + -3.1 616,629 + +0.4 616,630 - +2.4 616,640 + -1.7 616,679 - -0.4 616,720 - +4.0 616,720 - -0.1 616,752 - +2.6 616,755 - -0.3 616,755 - -0.4 616,760 - -1.1 616,809 + +3.1 616,830 + +0.6 616,830 + -2.0 616,830 + +2.2 616,830 + -0.2 616,830 + +0.5 616,830 + +2.6 616,831 - -1.4 616,936 + LU632_RS03020 0.14 -0.9 616,948 + LU632_RS03020 0.15 +0.6 616,969 + LU632_RS03020 0.17 +0.3 616,970 - LU632_RS03020 0.17 +0.1 616,970 - LU632_RS03020 0.17 +1.3 616,970 - LU632_RS03020 0.17 +1.8 616,970 - LU632_RS03020 0.17 +2.4 617,025 - LU632_RS03020 0.23 +0.4 617,025 - LU632_RS03020 0.23 +1.4 617,025 - LU632_RS03020 0.23 -1.9 617,073 + LU632_RS03020 0.27 -2.1 617,095 + LU632_RS03020 0.29 +0.6 617,123 - LU632_RS03020 0.32 -1.6 617,128 - LU632_RS03020 0.32 -1.3 617,178 + LU632_RS03020 0.37 +0.3 617,179 - LU632_RS03020 0.37 -0.2 617,260 + LU632_RS03020 0.45 -0.8 617,311 + LU632_RS03020 0.50 -0.0 617,312 - LU632_RS03020 0.50 +0.3 617,370 + LU632_RS03020 0.56 -1.5 617,397 + LU632_RS03020 0.58 -2.3 617,471 - LU632_RS03020 0.65 +0.5 617,472 + LU632_RS03020 0.66 +0.4 617,472 + LU632_RS03020 0.66 +0.1 617,472 + LU632_RS03020 0.66 -0.4 617,472 + LU632_RS03020 0.66 +0.4 617,473 - LU632_RS03020 0.66 +0.5 617,473 - LU632_RS03020 0.66 -0.4 617,473 - LU632_RS03020 0.66 +0.1 617,518 + LU632_RS03020 0.70 +1.0 617,519 - LU632_RS03020 0.70 +0.5
Or see this region's nucleotide sequence