Experiment: Experiment:TE, Cucumber 5 Main Stem (TE-S-C5-MS)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS01140 and dpaA overlap by 30 nucleotides dpaA and dinB are separated by 235 nucleotides dinB and pepD are separated by 167 nucleotides
LU632_RS01140: LU632_RS01140 - class II glutamine amidotransferase, at 242,476 to 243,243
_RS01140
LU632_RS01145: dpaA - peptidoglycan meso-diaminopimelic acid protein amidase, at 243,214 to 243,957
dpaA
LU632_RS01150: dinB - DNA polymerase IV, at 244,193 to 245,248
dinB
LU632_RS01155: pepD - beta-Ala-His dipeptidase, at 245,416 to 246,873
pepD
Position (kb) 244
245
246 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 243.274 kb on - strand at 243.290 kb on - strand, within dpaA at 243.294 kb on - strand, within dpaA at 243.492 kb on - strand, within dpaA at 243.492 kb on - strand, within dpaA at 243.495 kb on + strand, within dpaA at 243.495 kb on + strand, within dpaA at 243.496 kb on - strand, within dpaA at 243.547 kb on - strand, within dpaA at 243.564 kb on - strand, within dpaA at 243.601 kb on - strand, within dpaA at 243.907 kb on + strand at 243.975 kb on + strand at 243.975 kb on + strand at 243.987 kb on + strand at 243.988 kb on - strand at 243.988 kb on - strand at 243.988 kb on - strand at 244.034 kb on - strand at 244.051 kb on - strand at 244.198 kb on + strand at 244.210 kb on + strand at 244.210 kb on + strand at 244.324 kb on + strand, within dinB at 244.352 kb on + strand, within dinB at 244.353 kb on - strand, within dinB at 244.427 kb on + strand, within dinB at 244.428 kb on - strand, within dinB at 244.429 kb on + strand, within dinB at 244.430 kb on - strand, within dinB at 244.448 kb on - strand, within dinB at 244.508 kb on + strand, within dinB at 244.508 kb on + strand, within dinB at 244.509 kb on - strand, within dinB at 244.518 kb on + strand, within dinB at 244.518 kb on + strand, within dinB at 244.519 kb on - strand, within dinB at 244.519 kb on - strand, within dinB at 244.539 kb on - strand, within dinB at 244.580 kb on - strand, within dinB at 244.948 kb on + strand, within dinB at 245.056 kb on - strand, within dinB at 245.430 kb on + strand at 245.430 kb on + strand at 245.431 kb on - strand at 245.431 kb on - strand at 245.462 kb on + strand at 245.481 kb on + strand at 245.481 kb on + strand at 245.485 kb on + strand at 245.485 kb on + strand at 245.485 kb on + strand at 245.486 kb on - strand at 245.486 kb on - strand at 245.486 kb on - strand at 245.486 kb on - strand at 245.486 kb on - strand at 245.563 kb on - strand, within pepD at 245.613 kb on + strand, within pepD at 245.753 kb on + strand, within pepD at 245.753 kb on + strand, within pepD at 245.753 kb on + strand, within pepD at 245.794 kb on - strand, within pepD at 246.157 kb on + strand, within pepD at 246.157 kb on + strand, within pepD at 246.157 kb on + strand, within pepD at 246.189 kb on + strand, within pepD at 246.216 kb on + strand, within pepD at 246.216 kb on + strand, within pepD at 246.217 kb on - strand, within pepD
Per-strain Table
Position Strand Gene LocusTag Fraction Experiment:TE, Cucumber 5 Main Stem (TE-S-C5-MS) remove 243,274 - -1.5 243,290 - dpaA LU632_RS01145 0.10 -1.1 243,294 - dpaA LU632_RS01145 0.11 -0.9 243,492 - dpaA LU632_RS01145 0.37 +1.7 243,492 - dpaA LU632_RS01145 0.37 -0.9 243,495 + dpaA LU632_RS01145 0.38 +2.0 243,495 + dpaA LU632_RS01145 0.38 -1.2 243,496 - dpaA LU632_RS01145 0.38 -0.1 243,547 - dpaA LU632_RS01145 0.45 -2.6 243,564 - dpaA LU632_RS01145 0.47 -0.3 243,601 - dpaA LU632_RS01145 0.52 -0.7 243,907 + -1.9 243,975 + -2.1 243,975 + -0.9 243,987 + -3.6 243,988 - +1.0 243,988 - -1.3 243,988 - +1.3 244,034 - -3.1 244,051 - +2.1 244,198 + +0.7 244,210 + -0.7 244,210 + -0.5 244,324 + dinB LU632_RS01150 0.12 +2.1 244,352 + dinB LU632_RS01150 0.15 -0.5 244,353 - dinB LU632_RS01150 0.15 +0.3 244,427 + dinB LU632_RS01150 0.22 -0.5 244,428 - dinB LU632_RS01150 0.22 -1.1 244,429 + dinB LU632_RS01150 0.22 -1.1 244,430 - dinB LU632_RS01150 0.22 -1.0 244,448 - dinB LU632_RS01150 0.24 +0.4 244,508 + dinB LU632_RS01150 0.30 +0.9 244,508 + dinB LU632_RS01150 0.30 +1.1 244,509 - dinB LU632_RS01150 0.30 +1.8 244,518 + dinB LU632_RS01150 0.31 -0.5 244,518 + dinB LU632_RS01150 0.31 +0.9 244,519 - dinB LU632_RS01150 0.31 -0.8 244,519 - dinB LU632_RS01150 0.31 +0.1 244,539 - dinB LU632_RS01150 0.33 -0.1 244,580 - dinB LU632_RS01150 0.37 -1.3 244,948 + dinB LU632_RS01150 0.71 +0.8 245,056 - dinB LU632_RS01150 0.82 -0.2 245,430 + -1.2 245,430 + -2.7 245,431 - -0.7 245,431 - -0.4 245,462 + -0.1 245,481 + +0.5 245,481 + -1.1 245,485 + +2.5 245,485 + -0.3 245,485 + +0.6 245,486 - -0.9 245,486 - -1.5 245,486 - -0.3 245,486 - +0.5 245,486 - +0.1 245,563 - pepD LU632_RS01155 0.10 +0.7 245,613 + pepD LU632_RS01155 0.14 +0.9 245,753 + pepD LU632_RS01155 0.23 +1.2 245,753 + pepD LU632_RS01155 0.23 +2.0 245,753 + pepD LU632_RS01155 0.23 -1.2 245,794 - pepD LU632_RS01155 0.26 -0.8 246,157 + pepD LU632_RS01155 0.51 -0.7 246,157 + pepD LU632_RS01155 0.51 +0.9 246,157 + pepD LU632_RS01155 0.51 +0.5 246,189 + pepD LU632_RS01155 0.53 +0.1 246,216 + pepD LU632_RS01155 0.55 -0.3 246,216 + pepD LU632_RS01155 0.55 -1.8 246,217 - pepD LU632_RS01155 0.55 -1.3
Or see this region's nucleotide sequence