Experiment: Experiment:TE, Cucumber 5 Main Stem (TE-S-C5-MS)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rnhA and dnaQ are separated by 51 nucleotides dnaQ and LU632_RS01100 are separated by 122 nucleotides LU632_RS01100 and mtnC are separated by 475 nucleotides mtnC and LU632_RS01110 overlap by 29 nucleotides
LU632_RS01090: rnhA - ribonuclease HI, at 234,595 to 235,062
rnhA
LU632_RS01095: dnaQ - DNA polymerase III subunit epsilon, at 235,114 to 235,848
dnaQ
LU632_RS01100: LU632_RS01100 - tRNA-Asp, at 235,971 to 236,047
_RS01100
LU632_RS01105: mtnC - acireductone synthase, at 236,523 to 236,964
mtnC
LU632_RS01110: LU632_RS01110 - acireductone dioxygenase, at 236,936 to 237,407
_RS01110
Position (kb) 235
236
237 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 235.102 kb on + strand at 235.850 kb on + strand at 235.851 kb on - strand at 235.889 kb on + strand at 235.889 kb on + strand at 235.890 kb on - strand at 235.938 kb on - strand at 236.109 kb on + strand at 236.110 kb on - strand at 236.110 kb on - strand at 236.158 kb on + strand at 236.291 kb on - strand at 236.359 kb on + strand at 236.360 kb on - strand at 236.378 kb on + strand at 236.379 kb on - strand at 236.379 kb on - strand at 236.425 kb on + strand at 236.454 kb on + strand at 236.455 kb on - strand at 236.457 kb on - strand at 236.457 kb on - strand at 236.551 kb on + strand at 236.590 kb on + strand, within mtnC at 236.590 kb on + strand, within mtnC at 236.619 kb on + strand, within mtnC at 236.620 kb on - strand, within mtnC at 236.631 kb on + strand, within mtnC at 236.631 kb on + strand, within mtnC at 236.632 kb on - strand, within mtnC at 236.632 kb on - strand, within mtnC at 236.675 kb on + strand, within mtnC at 236.680 kb on - strand, within mtnC at 236.699 kb on + strand, within mtnC at 236.699 kb on + strand, within mtnC at 236.700 kb on - strand, within mtnC at 236.700 kb on - strand, within mtnC at 236.700 kb on - strand, within mtnC at 236.802 kb on + strand, within mtnC at 236.803 kb on - strand, within mtnC at 236.804 kb on + strand, within mtnC at 236.804 kb on + strand, within mtnC at 236.804 kb on + strand, within mtnC at 236.805 kb on - strand, within mtnC at 236.805 kb on - strand, within mtnC at 236.805 kb on - strand, within mtnC at 236.805 kb on - strand, within mtnC at 236.844 kb on + strand, within mtnC at 236.845 kb on - strand, within mtnC at 236.849 kb on + strand, within mtnC at 236.849 kb on + strand, within mtnC at 236.850 kb on - strand, within mtnC
Per-strain Table
Position Strand Gene LocusTag Fraction Experiment:TE, Cucumber 5 Main Stem (TE-S-C5-MS) remove 235,102 + +0.6 235,850 + +0.9 235,851 - -0.1 235,889 + +1.6 235,889 + +1.6 235,890 - +3.5 235,938 - +2.8 236,109 + -0.3 236,110 - +1.7 236,110 - +2.1 236,158 + +0.4 236,291 - -0.9 236,359 + +1.0 236,360 - +1.1 236,378 + +2.5 236,379 - +1.5 236,379 - +1.5 236,425 + -0.6 236,454 + +1.9 236,455 - -0.9 236,457 - -1.9 236,457 - -0.5 236,551 + -0.8 236,590 + mtnC LU632_RS01105 0.15 +0.4 236,590 + mtnC LU632_RS01105 0.15 +0.5 236,619 + mtnC LU632_RS01105 0.22 +0.9 236,620 - mtnC LU632_RS01105 0.22 -3.4 236,631 + mtnC LU632_RS01105 0.24 +0.4 236,631 + mtnC LU632_RS01105 0.24 -0.0 236,632 - mtnC LU632_RS01105 0.25 +1.9 236,632 - mtnC LU632_RS01105 0.25 +1.7 236,675 + mtnC LU632_RS01105 0.34 -1.0 236,680 - mtnC LU632_RS01105 0.36 -2.4 236,699 + mtnC LU632_RS01105 0.40 -0.9 236,699 + mtnC LU632_RS01105 0.40 -0.2 236,700 - mtnC LU632_RS01105 0.40 -0.2 236,700 - mtnC LU632_RS01105 0.40 -1.2 236,700 - mtnC LU632_RS01105 0.40 -2.6 236,802 + mtnC LU632_RS01105 0.63 +1.1 236,803 - mtnC LU632_RS01105 0.63 +1.2 236,804 + mtnC LU632_RS01105 0.64 -0.4 236,804 + mtnC LU632_RS01105 0.64 -1.6 236,804 + mtnC LU632_RS01105 0.64 +1.5 236,805 - mtnC LU632_RS01105 0.64 -1.6 236,805 - mtnC LU632_RS01105 0.64 -0.5 236,805 - mtnC LU632_RS01105 0.64 -0.5 236,805 - mtnC LU632_RS01105 0.64 -0.3 236,844 + mtnC LU632_RS01105 0.73 +0.3 236,845 - mtnC LU632_RS01105 0.73 -2.3 236,849 + mtnC LU632_RS01105 0.74 -1.7 236,849 + mtnC LU632_RS01105 0.74 +0.5 236,850 - mtnC LU632_RS01105 0.74 +0.5
Or see this region's nucleotide sequence