Strain Fitness in Erwinia tracheiphila SCR3 around LU632_RS10730

Experiment: Experiment:TE, Cucumber 5 Petiole 2 (TE-S-C5-P2)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntispE and lolB overlap by 4 nucleotideslolB and hemA are separated by 226 nucleotideshemA and prfA are separated by 37 nucleotides LU632_RS10720: ispE - 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase, at 2,043,593 to 2,044,498 ispE LU632_RS10725: lolB - lipoprotein insertase outer membrane protein LolB, at 2,044,495 to 2,045,118 lolB LU632_RS10730: hemA - glutamyl-tRNA reductase, at 2,045,345 to 2,046,601 hemA LU632_RS10735: prfA - peptide chain release factor 1, at 2,046,639 to 2,047,721 prfA Position (kb) 2045 2046 2047Strain fitness (log2 ratio) -1 0 1at 2045.213 kb on - strandat 2045.218 kb on - strandat 2045.218 kb on - strandat 2045.258 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Experiment:TE, Cucumber 5 Petiole 2 (TE-S-C5-P2)
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2,045,213 - +0.2
2,045,218 - +0.8
2,045,218 - -0.4
2,045,258 + +0.3

Or see this region's nucleotide sequence