Experiment: Experiment:TE, Cucumber 5 Petiole 2 (TE-S-C5-P2)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cysC and LU632_RS06440 are separated by 55 nucleotides LU632_RS06440 and ftsB are separated by 185 nucleotides ftsB and ispD are separated by 3 nucleotides ispD and ispF overlap by 1 nucleotides ispF and truD overlap by 1 nucleotides
LU632_RS06435: cysC - adenylyl-sulfate kinase, at 1,238,565 to 1,239,170
cysC
LU632_RS06440: LU632_RS06440 - DUF3561 family protein, at 1,239,226 to 1,239,546
_RS06440
LU632_RS06445: ftsB - cell division protein FtsB, at 1,239,732 to 1,240,052
ftsB
LU632_RS06450: ispD - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, at 1,240,056 to 1,240,772
ispD
LU632_RS06455: ispF - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, at 1,240,772 to 1,241,251
ispF
LU632_RS06460: truD - tRNA pseudouridine(13) synthase TruD, at 1,241,251 to 1,242,300
truD
Position (kb) 1240
1241 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1239.061 kb on - strand, within cysC at 1239.061 kb on - strand, within cysC at 1239.086 kb on - strand, within cysC at 1239.086 kb on - strand, within cysC at 1239.159 kb on - strand at 1239.221 kb on + strand at 1239.225 kb on + strand at 1239.225 kb on + strand at 1239.225 kb on + strand at 1239.225 kb on + strand at 1239.225 kb on + strand at 1239.225 kb on + strand at 1239.226 kb on - strand at 1239.226 kb on - strand at 1239.226 kb on - strand at 1239.366 kb on + strand, within LU632_RS06440 at 1239.385 kb on + strand, within LU632_RS06440 at 1239.385 kb on + strand, within LU632_RS06440 at 1239.387 kb on + strand, within LU632_RS06440 at 1239.388 kb on - strand, within LU632_RS06440 at 1239.469 kb on - strand, within LU632_RS06440 at 1239.469 kb on - strand, within LU632_RS06440 at 1239.550 kb on - strand at 1239.550 kb on - strand at 1239.553 kb on + strand at 1239.554 kb on - strand at 1239.554 kb on - strand at 1239.554 kb on - strand at 1239.659 kb on - strand at 1239.685 kb on + strand at 1240.754 kb on + strand at 1240.754 kb on + strand at 1240.757 kb on + strand at 1241.250 kb on - strand at 1241.306 kb on + strand at 1241.350 kb on + strand at 1241.448 kb on + strand, within truD at 1241.448 kb on + strand, within truD at 1241.449 kb on - strand, within truD at 1241.449 kb on - strand, within truD at 1241.523 kb on + strand, within truD at 1241.532 kb on + strand, within truD at 1241.550 kb on + strand, within truD at 1241.652 kb on - strand, within truD at 1241.719 kb on - strand, within truD at 1241.722 kb on + strand, within truD at 1241.723 kb on - strand, within truD at 1241.723 kb on - strand, within truD at 1241.766 kb on + strand, within truD
Per-strain Table
Position Strand Gene LocusTag Fraction Experiment:TE, Cucumber 5 Petiole 2 (TE-S-C5-P2) remove 1,239,061 - cysC LU632_RS06435 0.82 -0.8 1,239,061 - cysC LU632_RS06435 0.82 +2.0 1,239,086 - cysC LU632_RS06435 0.86 -0.6 1,239,086 - cysC LU632_RS06435 0.86 -1.6 1,239,159 - +0.4 1,239,221 + -1.3 1,239,225 + +1.2 1,239,225 + -0.8 1,239,225 + +0.1 1,239,225 + +0.2 1,239,225 + +0.2 1,239,225 + +0.7 1,239,226 - +0.0 1,239,226 - -0.7 1,239,226 - +0.7 1,239,366 + LU632_RS06440 0.44 -0.2 1,239,385 + LU632_RS06440 0.50 -2.0 1,239,385 + LU632_RS06440 0.50 +0.4 1,239,387 + LU632_RS06440 0.50 +0.9 1,239,388 - LU632_RS06440 0.50 +0.7 1,239,469 - LU632_RS06440 0.76 -0.1 1,239,469 - LU632_RS06440 0.76 +0.3 1,239,550 - -0.8 1,239,550 - +1.1 1,239,553 + +0.9 1,239,554 - -0.7 1,239,554 - -0.4 1,239,554 - +0.9 1,239,659 - +0.5 1,239,685 + -2.3 1,240,754 + +1.1 1,240,754 + -0.7 1,240,757 + +0.6 1,241,250 - -1.9 1,241,306 + +1.3 1,241,350 + +1.1 1,241,448 + truD LU632_RS06460 0.19 -1.2 1,241,448 + truD LU632_RS06460 0.19 +0.4 1,241,449 - truD LU632_RS06460 0.19 +1.0 1,241,449 - truD LU632_RS06460 0.19 -0.4 1,241,523 + truD LU632_RS06460 0.26 +1.3 1,241,532 + truD LU632_RS06460 0.27 +2.3 1,241,550 + truD LU632_RS06460 0.28 +0.7 1,241,652 - truD LU632_RS06460 0.38 -3.2 1,241,719 - truD LU632_RS06460 0.45 -0.6 1,241,722 + truD LU632_RS06460 0.45 +1.8 1,241,723 - truD LU632_RS06460 0.45 +0.6 1,241,723 - truD LU632_RS06460 0.45 +0.1 1,241,766 + truD LU632_RS06460 0.49 -0.8
Or see this region's nucleotide sequence