Experiment: Experiment:TE, Cucumber 5 Petiole 2 (TE-S-C5-P2)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thrA and thrB are separated by 2 nucleotides thrB and thrC are separated by 3 nucleotides
LU632_RS02610: thrA - bifunctional aspartate kinase/homoserine dehydrogenase I, at 520,215 to 522,677
thrA
LU632_RS02615: thrB - homoserine kinase, at 522,680 to 523,609
thrB
LU632_RS02620: thrC - threonine synthase, at 523,613 to 524,899
thrC
Position (kb) 522
523
524 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 521.700 kb on - strand, within thrA at 521.700 kb on - strand, within thrA at 521.855 kb on + strand, within thrA at 521.905 kb on - strand, within thrA at 521.976 kb on + strand, within thrA at 521.976 kb on + strand, within thrA at 521.977 kb on - strand, within thrA at 521.977 kb on - strand, within thrA at 521.977 kb on - strand, within thrA at 521.989 kb on + strand, within thrA at 521.989 kb on + strand, within thrA at 521.989 kb on + strand, within thrA at 521.994 kb on + strand, within thrA at 522.061 kb on + strand, within thrA at 522.140 kb on + strand, within thrA at 522.141 kb on - strand, within thrA at 522.141 kb on - strand, within thrA at 522.166 kb on + strand, within thrA at 522.167 kb on - strand, within thrA at 522.435 kb on - strand at 522.519 kb on + strand at 522.519 kb on + strand at 522.650 kb on + strand at 522.651 kb on - strand at 522.723 kb on + strand at 522.735 kb on + strand at 522.787 kb on + strand, within thrB at 522.842 kb on - strand, within thrB at 522.869 kb on - strand, within thrB at 522.869 kb on - strand, within thrB at 523.016 kb on + strand, within thrB at 523.016 kb on + strand, within thrB at 523.017 kb on - strand, within thrB at 523.017 kb on - strand, within thrB at 523.219 kb on + strand, within thrB at 523.219 kb on + strand, within thrB at 523.262 kb on + strand, within thrB at 523.263 kb on - strand, within thrB at 523.263 kb on - strand, within thrB at 523.384 kb on + strand, within thrB at 523.427 kb on - strand, within thrB at 523.432 kb on + strand, within thrB at 523.499 kb on - strand, within thrB at 523.539 kb on - strand at 523.539 kb on - strand at 523.568 kb on - strand at 523.598 kb on - strand at 523.970 kb on + strand, within thrC
Per-strain Table
Position Strand Gene LocusTag Fraction Experiment:TE, Cucumber 5 Petiole 2 (TE-S-C5-P2) remove 521,700 - thrA LU632_RS02610 0.60 -1.6 521,700 - thrA LU632_RS02610 0.60 -1.5 521,855 + thrA LU632_RS02610 0.67 -2.5 521,905 - thrA LU632_RS02610 0.69 -1.3 521,976 + thrA LU632_RS02610 0.71 -0.5 521,976 + thrA LU632_RS02610 0.71 -2.4 521,977 - thrA LU632_RS02610 0.72 -0.5 521,977 - thrA LU632_RS02610 0.72 -1.9 521,977 - thrA LU632_RS02610 0.72 +1.8 521,989 + thrA LU632_RS02610 0.72 -0.4 521,989 + thrA LU632_RS02610 0.72 -2.0 521,989 + thrA LU632_RS02610 0.72 +0.9 521,994 + thrA LU632_RS02610 0.72 -1.5 522,061 + thrA LU632_RS02610 0.75 -1.2 522,140 + thrA LU632_RS02610 0.78 -0.0 522,141 - thrA LU632_RS02610 0.78 -0.2 522,141 - thrA LU632_RS02610 0.78 -0.8 522,166 + thrA LU632_RS02610 0.79 -0.8 522,167 - thrA LU632_RS02610 0.79 -2.9 522,435 - -1.0 522,519 + -1.7 522,519 + -0.3 522,650 + +0.8 522,651 - -2.3 522,723 + -0.2 522,735 + -0.2 522,787 + thrB LU632_RS02615 0.12 -2.8 522,842 - thrB LU632_RS02615 0.17 -3.5 522,869 - thrB LU632_RS02615 0.20 -1.6 522,869 - thrB LU632_RS02615 0.20 +1.8 523,016 + thrB LU632_RS02615 0.36 -2.2 523,016 + thrB LU632_RS02615 0.36 -1.2 523,017 - thrB LU632_RS02615 0.36 -2.3 523,017 - thrB LU632_RS02615 0.36 -0.5 523,219 + thrB LU632_RS02615 0.58 -2.6 523,219 + thrB LU632_RS02615 0.58 -1.4 523,262 + thrB LU632_RS02615 0.63 -3.0 523,263 - thrB LU632_RS02615 0.63 -0.2 523,263 - thrB LU632_RS02615 0.63 -1.3 523,384 + thrB LU632_RS02615 0.76 +0.4 523,427 - thrB LU632_RS02615 0.80 -0.2 523,432 + thrB LU632_RS02615 0.81 -1.8 523,499 - thrB LU632_RS02615 0.88 -1.9 523,539 - -1.2 523,539 - -0.8 523,568 - -3.1 523,598 - -0.5 523,970 + thrC LU632_RS02620 0.28 +0.8
Or see this region's nucleotide sequence