Experiment: Bas55
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Arg_trna and HEPCGN_25305 are separated by 974 nucleotides HEPCGN_25305 and HEPCGN_25310 are separated by 150 nucleotides HEPCGN_25310 and HEPCGN_25315 are separated by 4 nucleotides HEPCGN_25315 and HEPCGN_25320 overlap by 35 nucleotides
HEPCGN_25290: Arg_trna - tRNA-Arg(tct), at 3,802,544 to 3,802,620
_trna
HEPCGN_25305: HEPCGN_25305 - 9-O-acetyl-N-acetylneuraminic acid deacetylase, at 3,803,595 to 3,805,448
_25305
HEPCGN_25310: HEPCGN_25310 - holin, at 3,805,599 to 3,805,814
_25310
HEPCGN_25315: HEPCGN_25315 - DUF1327 domain-containing protein, at 3,805,819 to 3,806,163
_25315
HEPCGN_25320: HEPCGN_25320 - Ybl58, at 3,806,129 to 3,806,401
_25320
Position (kb)
3803
3804
3805
3806 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3802.609 kb on + strand, within Arg_trna at 3802.626 kb on - strand at 3802.764 kb on - strand at 3802.764 kb on - strand at 3802.767 kb on + strand at 3802.767 kb on + strand at 3802.768 kb on - strand at 3802.769 kb on + strand at 3802.889 kb on - strand at 3802.889 kb on - strand at 3802.910 kb on + strand at 3802.917 kb on + strand at 3802.917 kb on + strand at 3802.917 kb on + strand at 3802.918 kb on - strand at 3802.918 kb on - strand at 3802.922 kb on + strand at 3802.937 kb on + strand at 3803.126 kb on + strand at 3803.127 kb on - strand at 3803.233 kb on + strand at 3803.233 kb on + strand at 3803.236 kb on - strand at 3803.411 kb on + strand at 3803.419 kb on - strand at 3803.430 kb on + strand at 3803.715 kb on + strand at 3803.716 kb on - strand at 3803.718 kb on - strand at 3803.787 kb on + strand, within HEPCGN_25305 at 3803.787 kb on + strand, within HEPCGN_25305 at 3803.787 kb on + strand, within HEPCGN_25305 at 3803.788 kb on - strand, within HEPCGN_25305 at 3803.788 kb on - strand, within HEPCGN_25305 at 3803.924 kb on - strand, within HEPCGN_25305 at 3803.990 kb on + strand, within HEPCGN_25305 at 3804.046 kb on + strand, within HEPCGN_25305 at 3804.046 kb on + strand, within HEPCGN_25305 at 3804.046 kb on + strand, within HEPCGN_25305 at 3804.046 kb on + strand, within HEPCGN_25305 at 3804.047 kb on - strand, within HEPCGN_25305 at 3804.072 kb on + strand, within HEPCGN_25305 at 3804.073 kb on - strand, within HEPCGN_25305 at 3804.104 kb on - strand, within HEPCGN_25305 at 3804.213 kb on + strand, within HEPCGN_25305 at 3804.321 kb on + strand, within HEPCGN_25305 at 3804.355 kb on + strand, within HEPCGN_25305 at 3804.355 kb on + strand, within HEPCGN_25305 at 3804.448 kb on - strand, within HEPCGN_25305 at 3804.471 kb on - strand, within HEPCGN_25305 at 3804.488 kb on - strand, within HEPCGN_25305 at 3804.489 kb on + strand, within HEPCGN_25305 at 3804.490 kb on - strand, within HEPCGN_25305 at 3804.498 kb on + strand, within HEPCGN_25305 at 3804.499 kb on - strand, within HEPCGN_25305 at 3804.499 kb on - strand, within HEPCGN_25305 at 3804.501 kb on - strand, within HEPCGN_25305 at 3804.501 kb on - strand, within HEPCGN_25305 at 3804.501 kb on - strand, within HEPCGN_25305 at 3804.506 kb on + strand, within HEPCGN_25305 at 3804.540 kb on - strand, within HEPCGN_25305 at 3804.616 kb on - strand, within HEPCGN_25305 at 3804.698 kb on + strand, within HEPCGN_25305 at 3804.710 kb on + strand, within HEPCGN_25305 at 3804.723 kb on - strand, within HEPCGN_25305 at 3804.723 kb on - strand, within HEPCGN_25305 at 3804.926 kb on + strand, within HEPCGN_25305 at 3805.228 kb on - strand, within HEPCGN_25305 at 3805.374 kb on - strand at 3805.392 kb on + strand at 3805.427 kb on - strand at 3805.447 kb on - strand at 3805.768 kb on - strand, within HEPCGN_25310 at 3806.167 kb on + strand, within HEPCGN_25320 at 3806.353 kb on + strand, within HEPCGN_25320 at 3806.392 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas55 remove 3,802,609 + Arg_trna HEPCGN_25290 0.84 +0.8 3,802,626 - -0.2 3,802,764 - -1.1 3,802,764 - -1.2 3,802,767 + -0.1 3,802,767 + +0.1 3,802,768 - +0.1 3,802,769 + -0.2 3,802,889 - +0.0 3,802,889 - +0.2 3,802,910 + -1.3 3,802,917 + +0.0 3,802,917 + +0.2 3,802,917 + +0.5 3,802,918 - +2.5 3,802,918 - -0.4 3,802,922 + -1.0 3,802,937 + -0.1 3,803,126 + +0.1 3,803,127 - -0.1 3,803,233 + +0.1 3,803,233 + +0.1 3,803,236 - -0.2 3,803,411 + -0.5 3,803,419 - +0.4 3,803,430 + +0.3 3,803,715 + +0.9 3,803,716 - +0.7 3,803,718 - -0.4 3,803,787 + HEPCGN_25305 0.10 +0.1 3,803,787 + HEPCGN_25305 0.10 -0.4 3,803,787 + HEPCGN_25305 0.10 -0.8 3,803,788 - HEPCGN_25305 0.10 +0.7 3,803,788 - HEPCGN_25305 0.10 +0.5 3,803,924 - HEPCGN_25305 0.18 -0.2 3,803,990 + HEPCGN_25305 0.21 -0.8 3,804,046 + HEPCGN_25305 0.24 +0.9 3,804,046 + HEPCGN_25305 0.24 -0.4 3,804,046 + HEPCGN_25305 0.24 +0.0 3,804,046 + HEPCGN_25305 0.24 -0.0 3,804,047 - HEPCGN_25305 0.24 -1.6 3,804,072 + HEPCGN_25305 0.26 +0.1 3,804,073 - HEPCGN_25305 0.26 +0.8 3,804,104 - HEPCGN_25305 0.27 +2.1 3,804,213 + HEPCGN_25305 0.33 -1.5 3,804,321 + HEPCGN_25305 0.39 -1.3 3,804,355 + HEPCGN_25305 0.41 +0.4 3,804,355 + HEPCGN_25305 0.41 -0.3 3,804,448 - HEPCGN_25305 0.46 -0.2 3,804,471 - HEPCGN_25305 0.47 -0.6 3,804,488 - HEPCGN_25305 0.48 -0.1 3,804,489 + HEPCGN_25305 0.48 +0.5 3,804,490 - HEPCGN_25305 0.48 +1.2 3,804,498 + HEPCGN_25305 0.49 +0.4 3,804,499 - HEPCGN_25305 0.49 -0.4 3,804,499 - HEPCGN_25305 0.49 -0.2 3,804,501 - HEPCGN_25305 0.49 -0.1 3,804,501 - HEPCGN_25305 0.49 +0.8 3,804,501 - HEPCGN_25305 0.49 +1.5 3,804,506 + HEPCGN_25305 0.49 -0.5 3,804,540 - HEPCGN_25305 0.51 -0.6 3,804,616 - HEPCGN_25305 0.55 +1.4 3,804,698 + HEPCGN_25305 0.59 +0.1 3,804,710 + HEPCGN_25305 0.60 +0.7 3,804,723 - HEPCGN_25305 0.61 +0.2 3,804,723 - HEPCGN_25305 0.61 -0.1 3,804,926 + HEPCGN_25305 0.72 +0.2 3,805,228 - HEPCGN_25305 0.88 +0.0 3,805,374 - -1.9 3,805,392 + -1.3 3,805,427 - -0.8 3,805,447 - -0.1 3,805,768 - HEPCGN_25310 0.78 -0.2 3,806,167 + HEPCGN_25320 0.14 -0.5 3,806,353 + HEPCGN_25320 0.82 -0.8 3,806,392 + +0.3
Or see this region's nucleotide sequence