Experiment: Bas55
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt plsY and folB are separated by 104 nucleotides folB and bacA are separated by 90 nucleotides bacA and cca are separated by 180 nucleotides
HEPCGN_17550: plsY - glycerol-3-phosphate 1-O-acyltransferase PlsY, at 2,259,043 to 2,259,660
plsY
HEPCGN_17555: folB - bifunctional dihydroneopterin aldolase/7,8-dihydroneopterin epimerase, at 2,259,765 to 2,260,133
folB
HEPCGN_17560: bacA - undecaprenyl-diphosphate phosphatase, at 2,260,224 to 2,261,045
bacA
HEPCGN_17570: cca - fused tRNA nucleotidyltransferase/2',3'-cyclic phosphodiesterase/2' nucleotidase/phosphatase Cca, at 2,261,226 to 2,262,464
cca
Position (kb)
2260
2261
2262 Strain fitness (log2 ratio)
-2
-1
0
1 at 2259.406 kb on + strand, within plsY at 2259.407 kb on - strand, within plsY at 2259.413 kb on - strand, within plsY at 2259.433 kb on + strand, within plsY at 2259.440 kb on + strand, within plsY at 2259.516 kb on + strand, within plsY at 2259.516 kb on + strand, within plsY at 2259.520 kb on + strand, within plsY at 2259.520 kb on + strand, within plsY at 2259.521 kb on - strand, within plsY at 2259.660 kb on + strand at 2259.661 kb on - strand at 2259.710 kb on + strand at 2259.711 kb on - strand at 2260.113 kb on - strand at 2260.172 kb on + strand at 2260.180 kb on + strand at 2260.206 kb on + strand at 2260.207 kb on - strand at 2260.233 kb on - strand at 2260.313 kb on + strand, within bacA at 2260.319 kb on + strand, within bacA at 2260.320 kb on - strand, within bacA at 2260.320 kb on - strand, within bacA at 2260.406 kb on - strand, within bacA at 2260.562 kb on + strand, within bacA at 2260.593 kb on + strand, within bacA at 2260.593 kb on + strand, within bacA at 2260.594 kb on - strand, within bacA at 2260.676 kb on + strand, within bacA at 2260.676 kb on + strand, within bacA at 2260.676 kb on + strand, within bacA at 2260.683 kb on + strand, within bacA at 2260.698 kb on - strand, within bacA at 2260.792 kb on - strand, within bacA at 2260.860 kb on + strand, within bacA at 2260.917 kb on - strand, within bacA at 2260.949 kb on + strand, within bacA at 2261.002 kb on - strand at 2261.009 kb on + strand at 2261.028 kb on + strand at 2261.028 kb on + strand at 2261.028 kb on + strand at 2261.029 kb on - strand at 2261.054 kb on + strand at 2261.054 kb on + strand at 2261.055 kb on - strand at 2261.180 kb on + strand at 2261.767 kb on - strand, within cca
Per-strain Table
Position Strand Gene LocusTag Fraction Bas55 remove 2,259,406 + plsY HEPCGN_17550 0.59 +0.1 2,259,407 - plsY HEPCGN_17550 0.59 -0.1 2,259,413 - plsY HEPCGN_17550 0.60 -1.2 2,259,433 + plsY HEPCGN_17550 0.63 -0.2 2,259,440 + plsY HEPCGN_17550 0.64 +0.7 2,259,516 + plsY HEPCGN_17550 0.77 -1.9 2,259,516 + plsY HEPCGN_17550 0.77 +1.4 2,259,520 + plsY HEPCGN_17550 0.77 +0.1 2,259,520 + plsY HEPCGN_17550 0.77 -0.3 2,259,521 - plsY HEPCGN_17550 0.77 -1.2 2,259,660 + +0.4 2,259,661 - +1.1 2,259,710 + +0.7 2,259,711 - +0.6 2,260,113 - +0.3 2,260,172 + -1.0 2,260,180 + +0.5 2,260,206 + -0.0 2,260,207 - -0.4 2,260,233 - +1.6 2,260,313 + bacA HEPCGN_17560 0.11 +0.3 2,260,319 + bacA HEPCGN_17560 0.12 +0.0 2,260,320 - bacA HEPCGN_17560 0.12 +0.1 2,260,320 - bacA HEPCGN_17560 0.12 +1.4 2,260,406 - bacA HEPCGN_17560 0.22 +0.0 2,260,562 + bacA HEPCGN_17560 0.41 +0.1 2,260,593 + bacA HEPCGN_17560 0.45 -0.5 2,260,593 + bacA HEPCGN_17560 0.45 -0.3 2,260,594 - bacA HEPCGN_17560 0.45 +1.3 2,260,676 + bacA HEPCGN_17560 0.55 -0.2 2,260,676 + bacA HEPCGN_17560 0.55 -0.1 2,260,676 + bacA HEPCGN_17560 0.55 -0.4 2,260,683 + bacA HEPCGN_17560 0.56 -0.3 2,260,698 - bacA HEPCGN_17560 0.58 -0.4 2,260,792 - bacA HEPCGN_17560 0.69 +0.5 2,260,860 + bacA HEPCGN_17560 0.77 +0.3 2,260,917 - bacA HEPCGN_17560 0.84 +0.6 2,260,949 + bacA HEPCGN_17560 0.88 -0.4 2,261,002 - -0.1 2,261,009 + +0.2 2,261,028 + +0.7 2,261,028 + -0.3 2,261,028 + +0.2 2,261,029 - -0.0 2,261,054 + -0.0 2,261,054 + -0.2 2,261,055 - +0.2 2,261,180 + -0.3 2,261,767 - cca HEPCGN_17570 0.44 +1.1
Or see this region's nucleotide sequence