Experiment: Bas55
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt infB and rbfA are separated by 163 nucleotides rbfA and truB overlap by 1 nucleotides
HEPCGN_16985: infB - Translation initiation factor IF-2, at 2,145,829 to 2,148,501
infB
HEPCGN_16995: rbfA - 30S ribosome-binding factor RbfA, at 2,148,665 to 2,149,066
rbfA
HEPCGN_17000: truB - tRNA pseudouridine(55) synthase TruB, at 2,149,066 to 2,150,010
truB
Position (kb)
2148
2149
2150 Strain fitness (log2 ratio)
-1
0
1 at 2148.489 kb on + strand at 2148.499 kb on + strand at 2149.120 kb on - strand at 2149.171 kb on + strand, within truB at 2149.172 kb on - strand, within truB at 2149.268 kb on - strand, within truB at 2149.456 kb on + strand, within truB at 2149.457 kb on - strand, within truB at 2149.480 kb on + strand, within truB at 2149.545 kb on + strand, within truB at 2149.545 kb on + strand, within truB at 2149.600 kb on + strand, within truB at 2149.600 kb on + strand, within truB at 2149.600 kb on + strand, within truB at 2149.600 kb on + strand, within truB at 2149.600 kb on + strand, within truB at 2149.600 kb on + strand, within truB at 2149.601 kb on - strand, within truB at 2149.601 kb on - strand, within truB at 2149.601 kb on - strand, within truB at 2149.656 kb on + strand, within truB at 2149.656 kb on + strand, within truB at 2149.657 kb on - strand, within truB at 2149.657 kb on - strand, within truB at 2149.683 kb on + strand, within truB at 2149.684 kb on - strand, within truB at 2149.684 kb on - strand, within truB at 2149.772 kb on + strand, within truB at 2149.772 kb on + strand, within truB at 2149.878 kb on + strand, within truB
Per-strain Table
Position Strand Gene LocusTag Fraction Bas55 remove 2,148,489 + +0.7 2,148,499 + +0.5 2,149,120 - -0.1 2,149,171 + truB HEPCGN_17000 0.11 +0.0 2,149,172 - truB HEPCGN_17000 0.11 +0.2 2,149,268 - truB HEPCGN_17000 0.21 -0.5 2,149,456 + truB HEPCGN_17000 0.41 +1.0 2,149,457 - truB HEPCGN_17000 0.41 -0.6 2,149,480 + truB HEPCGN_17000 0.44 -1.1 2,149,545 + truB HEPCGN_17000 0.51 +1.4 2,149,545 + truB HEPCGN_17000 0.51 -0.7 2,149,600 + truB HEPCGN_17000 0.57 +0.2 2,149,600 + truB HEPCGN_17000 0.57 -0.3 2,149,600 + truB HEPCGN_17000 0.57 -0.1 2,149,600 + truB HEPCGN_17000 0.57 -0.5 2,149,600 + truB HEPCGN_17000 0.57 +1.1 2,149,600 + truB HEPCGN_17000 0.57 -1.6 2,149,601 - truB HEPCGN_17000 0.57 -0.2 2,149,601 - truB HEPCGN_17000 0.57 -0.7 2,149,601 - truB HEPCGN_17000 0.57 -0.3 2,149,656 + truB HEPCGN_17000 0.62 +0.6 2,149,656 + truB HEPCGN_17000 0.62 -0.2 2,149,657 - truB HEPCGN_17000 0.63 -0.4 2,149,657 - truB HEPCGN_17000 0.63 -0.8 2,149,683 + truB HEPCGN_17000 0.65 -0.8 2,149,684 - truB HEPCGN_17000 0.65 -0.1 2,149,684 - truB HEPCGN_17000 0.65 -0.2 2,149,772 + truB HEPCGN_17000 0.75 +1.3 2,149,772 + truB HEPCGN_17000 0.75 +0.2 2,149,878 + truB HEPCGN_17000 0.86 -0.4
Or see this region's nucleotide sequence