Strain Fitness in Escherichia coli ECOR38 around HEPCGN_12020

Experiment: Bas55

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntthiC and thiE overlap by 1 nucleotidesthiE and thiF overlap by 8 nucleotidesthiF and thiF overlap by 17 nucleotidesthiF and thiG are separated by 1 nucleotidesthiG and thiH overlap by 4 nucleotides HEPCGN_12010: thiC - phosphomethylpyrimidine synthase ThiC, at 1,141,535 to 1,143,430 thiC HEPCGN_12015: thiE - thiamine phosphate synthase, at 1,143,430 to 1,144,065 thiE HEPCGN_12020: thiF - thiazole biosynthesis adenylyltransferase ThiF, at 1,144,058 to 1,144,813 thiF HEPCGN_12025: thiF - thiazole biosynthesis adenylyltransferase ThiF, at 1,144,797 to 1,144,997 thiF HEPCGN_12030: thiG - thiazole synthase, at 1,144,999 to 1,145,769 thiG HEPCGN_12035: thiH - 2-iminoacetate synthase ThiH, at 1,145,766 to 1,146,899 thiH Position (kb) 1144 1145Strain fitness (log2 ratio) -2 -1 0 1at 1143.077 kb on - strand, within thiCat 1143.084 kb on - strand, within thiCat 1143.193 kb on + strand, within thiCat 1143.324 kb on + strandat 1143.324 kb on + strandat 1143.325 kb on - strandat 1143.325 kb on - strandat 1143.332 kb on + strandat 1143.332 kb on + strandat 1143.371 kb on - strandat 1143.456 kb on - strandat 1143.490 kb on - strandat 1143.494 kb on + strand, within thiEat 1143.494 kb on + strand, within thiEat 1143.495 kb on - strand, within thiEat 1143.670 kb on + strand, within thiEat 1143.671 kb on - strand, within thiEat 1143.802 kb on + strand, within thiEat 1143.802 kb on + strand, within thiEat 1143.802 kb on + strand, within thiEat 1143.803 kb on - strand, within thiEat 1143.803 kb on - strand, within thiEat 1143.804 kb on + strand, within thiEat 1143.804 kb on + strand, within thiEat 1143.805 kb on - strand, within thiEat 1143.996 kb on + strand, within thiEat 1143.997 kb on - strand, within thiEat 1143.997 kb on - strand, within thiEat 1144.082 kb on + strandat 1144.084 kb on + strandat 1144.085 kb on - strandat 1144.085 kb on - strandat 1144.193 kb on + strand, within thiFat 1144.194 kb on - strand, within thiFat 1144.224 kb on + strand, within thiFat 1144.252 kb on - strand, within thiFat 1144.279 kb on + strand, within thiFat 1144.365 kb on + strand, within thiFat 1144.365 kb on + strand, within thiFat 1144.365 kb on + strand, within thiFat 1144.366 kb on - strand, within thiFat 1144.392 kb on + strand, within thiFat 1144.392 kb on + strand, within thiFat 1144.495 kb on + strand, within thiFat 1144.496 kb on - strand, within thiFat 1144.536 kb on + strand, within thiFat 1144.646 kb on - strand, within thiFat 1144.680 kb on + strand, within thiFat 1144.691 kb on - strand, within thiFat 1144.693 kb on - strand, within thiFat 1144.693 kb on - strand, within thiFat 1144.944 kb on - strand, within thiFat 1144.944 kb on - strand, within thiFat 1145.003 kb on + strandat 1145.007 kb on + strandat 1145.008 kb on - strandat 1145.203 kb on - strand, within thiGat 1145.285 kb on + strand, within thiGat 1145.441 kb on + strand, within thiGat 1145.604 kb on - strand, within thiG

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas55
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1,143,077 - thiC HEPCGN_12010 0.81 -1.2
1,143,084 - thiC HEPCGN_12010 0.82 -0.3
1,143,193 + thiC HEPCGN_12010 0.87 -1.8
1,143,324 + -0.1
1,143,324 + -1.0
1,143,325 - -0.1
1,143,325 - +1.4
1,143,332 + -0.4
1,143,332 + +0.3
1,143,371 - -0.9
1,143,456 - -0.4
1,143,490 - +0.1
1,143,494 + thiE HEPCGN_12015 0.10 +0.2
1,143,494 + thiE HEPCGN_12015 0.10 +1.7
1,143,495 - thiE HEPCGN_12015 0.10 +0.4
1,143,670 + thiE HEPCGN_12015 0.38 +0.6
1,143,671 - thiE HEPCGN_12015 0.38 +0.3
1,143,802 + thiE HEPCGN_12015 0.58 -0.7
1,143,802 + thiE HEPCGN_12015 0.58 -0.1
1,143,802 + thiE HEPCGN_12015 0.58 +0.6
1,143,803 - thiE HEPCGN_12015 0.59 +0.2
1,143,803 - thiE HEPCGN_12015 0.59 -0.0
1,143,804 + thiE HEPCGN_12015 0.59 -0.2
1,143,804 + thiE HEPCGN_12015 0.59 +0.3
1,143,805 - thiE HEPCGN_12015 0.59 -0.8
1,143,996 + thiE HEPCGN_12015 0.89 -0.4
1,143,997 - thiE HEPCGN_12015 0.89 -0.3
1,143,997 - thiE HEPCGN_12015 0.89 +0.1
1,144,082 + -0.2
1,144,084 + -0.8
1,144,085 - +0.0
1,144,085 - -0.0
1,144,193 + thiF HEPCGN_12020 0.18 +0.7
1,144,194 - thiF HEPCGN_12020 0.18 +0.5
1,144,224 + thiF HEPCGN_12020 0.22 +0.2
1,144,252 - thiF HEPCGN_12020 0.26 -0.3
1,144,279 + thiF HEPCGN_12020 0.29 +0.4
1,144,365 + thiF HEPCGN_12020 0.41 +0.5
1,144,365 + thiF HEPCGN_12020 0.41 -0.2
1,144,365 + thiF HEPCGN_12020 0.41 +0.3
1,144,366 - thiF HEPCGN_12020 0.41 +0.6
1,144,392 + thiF HEPCGN_12020 0.44 -0.8
1,144,392 + thiF HEPCGN_12020 0.44 -0.2
1,144,495 + thiF HEPCGN_12020 0.58 -0.4
1,144,496 - thiF HEPCGN_12020 0.58 +0.1
1,144,536 + thiF HEPCGN_12020 0.63 +0.0
1,144,646 - thiF HEPCGN_12020 0.78 -0.6
1,144,680 + thiF HEPCGN_12020 0.82 +1.2
1,144,691 - thiF HEPCGN_12020 0.84 -0.2
1,144,693 - thiF HEPCGN_12020 0.84 +0.1
1,144,693 - thiF HEPCGN_12020 0.84 +0.2
1,144,944 - thiF HEPCGN_12025 0.73 -0.5
1,144,944 - thiF HEPCGN_12025 0.73 -0.4
1,145,003 + -0.4
1,145,007 + +0.4
1,145,008 - +1.2
1,145,203 - thiG HEPCGN_12030 0.26 -0.3
1,145,285 + thiG HEPCGN_12030 0.37 -0.3
1,145,441 + thiG HEPCGN_12030 0.57 +0.8
1,145,604 - thiG HEPCGN_12030 0.78 +0.6

Or see this region's nucleotide sequence