Experiment: R2A_PIPES with Metal_ion_mix_sitestressplate 2 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt queF and OKGIIK_13400 are separated by 188 nucleotides OKGIIK_13400 and OKGIIK_13405 overlap by 30 nucleotides OKGIIK_13405 and OKGIIK_13410 are separated by 47 nucleotides OKGIIK_13410 and OKGIIK_13415 are separated by 228 nucleotides
OKGIIK_13395: queF - NADPH-dependent 7-cyano-7-deazaguanine reductase QueF, at 2,893,751 to 2,894,575
queF
OKGIIK_13400: OKGIIK_13400 - hypothetical protein, at 2,894,764 to 2,895,375
_13400
OKGIIK_13405: OKGIIK_13405 - IS3 family transposase, at 2,895,346 to 2,896,164
_13405
OKGIIK_13410: OKGIIK_13410 - Mobile element protein, at 2,896,212 to 2,896,499
_13410
OKGIIK_13415: OKGIIK_13415 - SseB family protein, at 2,896,728 to 2,897,267
_13415
Position (kb)
2895
2896
2897 Strain fitness (log2 ratio)
-2
-1
0
1 at 2894.378 kb on + strand, within queF at 2894.480 kb on + strand, within queF at 2894.481 kb on - strand, within queF at 2894.481 kb on - strand, within queF at 2894.565 kb on + strand at 2894.565 kb on + strand at 2894.574 kb on - strand at 2894.785 kb on - strand at 2894.785 kb on - strand at 2895.055 kb on + strand, within OKGIIK_13400 at 2895.056 kb on - strand, within OKGIIK_13400 at 2895.056 kb on - strand, within OKGIIK_13400 at 2895.062 kb on + strand, within OKGIIK_13400 at 2895.062 kb on + strand, within OKGIIK_13400 at 2895.063 kb on - strand, within OKGIIK_13400 at 2895.106 kb on + strand, within OKGIIK_13400 at 2895.106 kb on + strand, within OKGIIK_13400 at 2895.106 kb on + strand, within OKGIIK_13400 at 2895.106 kb on + strand, within OKGIIK_13400 at 2895.106 kb on + strand, within OKGIIK_13400 at 2895.106 kb on + strand, within OKGIIK_13400 at 2895.107 kb on - strand, within OKGIIK_13400 at 2895.107 kb on - strand, within OKGIIK_13400 at 2895.107 kb on - strand, within OKGIIK_13400 at 2895.107 kb on - strand, within OKGIIK_13400 at 2895.107 kb on - strand, within OKGIIK_13400 at 2895.166 kb on + strand, within OKGIIK_13400 at 2895.166 kb on + strand, within OKGIIK_13400 at 2895.167 kb on - strand, within OKGIIK_13400 at 2895.167 kb on - strand, within OKGIIK_13400 at 2895.280 kb on + strand, within OKGIIK_13400 at 2895.280 kb on + strand, within OKGIIK_13400 at 2895.280 kb on + strand, within OKGIIK_13400 at 2895.281 kb on - strand, within OKGIIK_13400 at 2895.281 kb on - strand, within OKGIIK_13400 at 2895.374 kb on - strand at 2895.374 kb on - strand at 2895.374 kb on - strand at 2896.531 kb on + strand at 2896.531 kb on + strand at 2896.531 kb on + strand at 2896.531 kb on + strand at 2896.602 kb on + strand at 2896.647 kb on + strand at 2896.647 kb on + strand at 2896.647 kb on + strand at 2896.648 kb on - strand at 2896.657 kb on + strand at 2896.657 kb on + strand at 2896.657 kb on + strand at 2896.658 kb on - strand at 2896.658 kb on - strand at 2896.658 kb on - strand at 2896.658 kb on - strand at 2896.658 kb on - strand at 2896.706 kb on - strand at 2896.706 kb on - strand at 2896.707 kb on - strand at 2896.848 kb on - strand, within OKGIIK_13415 at 2896.848 kb on - strand, within OKGIIK_13415 at 2896.939 kb on + strand, within OKGIIK_13415 at 2896.939 kb on + strand, within OKGIIK_13415 at 2896.940 kb on - strand, within OKGIIK_13415 at 2896.994 kb on - strand, within OKGIIK_13415 at 2897.035 kb on + strand, within OKGIIK_13415 at 2897.035 kb on + strand, within OKGIIK_13415 at 2897.035 kb on + strand, within OKGIIK_13415
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Metal_ion_mix_sitestressplate 2 x remove 2,894,378 + queF OKGIIK_13395 0.76 -0.6 2,894,480 + queF OKGIIK_13395 0.88 -0.8 2,894,481 - queF OKGIIK_13395 0.88 -0.3 2,894,481 - queF OKGIIK_13395 0.88 +0.9 2,894,565 + +0.2 2,894,565 + +0.5 2,894,574 - +0.3 2,894,785 - +0.1 2,894,785 - +1.4 2,895,055 + OKGIIK_13400 0.48 -1.1 2,895,056 - OKGIIK_13400 0.48 -1.0 2,895,056 - OKGIIK_13400 0.48 +1.5 2,895,062 + OKGIIK_13400 0.49 -0.2 2,895,062 + OKGIIK_13400 0.49 +0.1 2,895,063 - OKGIIK_13400 0.49 -0.2 2,895,106 + OKGIIK_13400 0.56 +1.2 2,895,106 + OKGIIK_13400 0.56 +0.2 2,895,106 + OKGIIK_13400 0.56 -0.9 2,895,106 + OKGIIK_13400 0.56 -0.2 2,895,106 + OKGIIK_13400 0.56 -2.1 2,895,106 + OKGIIK_13400 0.56 +0.1 2,895,107 - OKGIIK_13400 0.56 +0.5 2,895,107 - OKGIIK_13400 0.56 +1.2 2,895,107 - OKGIIK_13400 0.56 -0.1 2,895,107 - OKGIIK_13400 0.56 -0.9 2,895,107 - OKGIIK_13400 0.56 -0.4 2,895,166 + OKGIIK_13400 0.66 +0.9 2,895,166 + OKGIIK_13400 0.66 -0.1 2,895,167 - OKGIIK_13400 0.66 -0.1 2,895,167 - OKGIIK_13400 0.66 -2.4 2,895,280 + OKGIIK_13400 0.84 -1.1 2,895,280 + OKGIIK_13400 0.84 -1.6 2,895,280 + OKGIIK_13400 0.84 -1.0 2,895,281 - OKGIIK_13400 0.84 -1.8 2,895,281 - OKGIIK_13400 0.84 -1.0 2,895,374 - +0.2 2,895,374 - +0.5 2,895,374 - -0.5 2,896,531 + -0.9 2,896,531 + -1.2 2,896,531 + -2.1 2,896,531 + +0.1 2,896,602 + -0.9 2,896,647 + +1.1 2,896,647 + -0.6 2,896,647 + +0.2 2,896,648 - +0.1 2,896,657 + +0.1 2,896,657 + +0.8 2,896,657 + -0.0 2,896,658 - -0.4 2,896,658 - -1.1 2,896,658 - +0.2 2,896,658 - -0.6 2,896,658 - -0.6 2,896,706 - -1.4 2,896,706 - +0.9 2,896,707 - +0.9 2,896,848 - OKGIIK_13415 0.22 +0.9 2,896,848 - OKGIIK_13415 0.22 -0.5 2,896,939 + OKGIIK_13415 0.39 -1.1 2,896,939 + OKGIIK_13415 0.39 -0.2 2,896,940 - OKGIIK_13415 0.39 +0.9 2,896,994 - OKGIIK_13415 0.49 +1.6 2,897,035 + OKGIIK_13415 0.57 -0.1 2,897,035 + OKGIIK_13415 0.57 +0.1 2,897,035 + OKGIIK_13415 0.57 -1.1
Or see this region's nucleotide sequence