Strain Fitness in Escherichia coli ECOR38 around HEPCGN_16980

Experiment: Bas55

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfMet_trna and rimP are separated by 206 nucleotidesrimP and nusA are separated by 27 nucleotidesnusA and infB are separated by 24 nucleotides HEPCGN_16970: fMet_trna - tRNA-fMet(cat), at 2,143,554 to 2,143,630 _trna HEPCGN_16975: rimP - ribosome maturation factor RimP, at 2,143,837 to 2,144,289 rimP HEPCGN_16980: nusA - Transcription termination/antitermination protein NusA, at 2,144,317 to 2,145,804 nusA HEPCGN_16985: infB - Translation initiation factor IF-2, at 2,145,829 to 2,148,501 infB Position (kb) 2144 2145 2146Strain fitness (log2 ratio) -1 0 1at 2143.378 kb on + strandat 2143.379 kb on - strandat 2143.406 kb on + strandat 2143.406 kb on + strandat 2143.407 kb on - strandat 2143.407 kb on - strandat 2143.575 kb on - strand, within fMet_trnaat 2143.736 kb on + strandat 2143.749 kb on + strandat 2143.751 kb on + strandat 2144.287 kb on + strandat 2144.287 kb on + strandat 2145.472 kb on + strand, within nusAat 2145.777 kb on + strandat 2145.777 kb on + strandat 2145.802 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas55
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2,143,378 + -0.2
2,143,379 - -0.2
2,143,406 + -0.5
2,143,406 + -0.7
2,143,407 - +0.8
2,143,407 - -0.6
2,143,575 - fMet_trna HEPCGN_16970 0.27 -1.4
2,143,736 + -0.4
2,143,749 + -1.0
2,143,751 + -1.2
2,144,287 + -0.9
2,144,287 + -0.7
2,145,472 + nusA HEPCGN_16980 0.78 -0.4
2,145,777 + +0.4
2,145,777 + +0.0
2,145,802 + +0.2

Or see this region's nucleotide sequence