Strain Fitness in Escherichia coli ECOR38 around HEPCGN_14520

Experiment: Bas55

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntHEPCGN_14510 and rfaY overlap by 4 nucleotidesrfaY and rfaJ are separated by 25 nucleotidesrfaJ and HEPCGN_14525 are separated by 78 nucleotides HEPCGN_14510: HEPCGN_14510 - IS4-like element ISVsa5 family transposase, at 1,655,203 to 1,656,411 _14510 HEPCGN_14515: rfaY - lipopolysaccharide core heptose(II) kinase RfaY, at 1,656,408 to 1,656,965 rfaY HEPCGN_14520: rfaJ - UDP-galactose--(galactosyl) LPS alpha1,2-galactosyltransferase, at 1,656,991 to 1,658,019 rfaJ HEPCGN_14525: HEPCGN_14525 - beta1,3-glucosyltransferase, at 1,658,098 to 1,659,081 _14525 Position (kb) 1656 1657 1658 1659Strain fitness (log2 ratio) -2 -1 0 1 2at 1656.433 kb on - strandat 1656.442 kb on - strandat 1656.612 kb on + strand, within rfaYat 1656.737 kb on + strand, within rfaYat 1656.737 kb on + strand, within rfaYat 1656.738 kb on - strand, within rfaYat 1656.738 kb on - strand, within rfaYat 1656.903 kb on + strand, within rfaYat 1656.915 kb on + strandat 1656.998 kb on + strandat 1657.113 kb on + strand, within rfaJat 1657.114 kb on - strand, within rfaJat 1657.213 kb on - strand, within rfaJat 1657.363 kb on + strand, within rfaJat 1657.852 kb on + strand, within rfaJat 1658.233 kb on - strand, within HEPCGN_14525at 1658.338 kb on - strand, within HEPCGN_14525at 1658.363 kb on + strand, within HEPCGN_14525at 1658.452 kb on + strand, within HEPCGN_14525at 1658.476 kb on - strand, within HEPCGN_14525at 1658.477 kb on + strand, within HEPCGN_14525at 1658.504 kb on + strand, within HEPCGN_14525at 1658.673 kb on - strand, within HEPCGN_14525at 1658.719 kb on - strand, within HEPCGN_14525at 1658.800 kb on + strand, within HEPCGN_14525at 1659.014 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas55
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1,656,433 - -0.3
1,656,442 - +0.7
1,656,612 + rfaY HEPCGN_14515 0.37 -1.0
1,656,737 + rfaY HEPCGN_14515 0.59 +0.1
1,656,737 + rfaY HEPCGN_14515 0.59 +0.4
1,656,738 - rfaY HEPCGN_14515 0.59 +1.9
1,656,738 - rfaY HEPCGN_14515 0.59 +0.5
1,656,903 + rfaY HEPCGN_14515 0.89 +0.3
1,656,915 + +0.9
1,656,998 + -0.4
1,657,113 + rfaJ HEPCGN_14520 0.12 -1.3
1,657,114 - rfaJ HEPCGN_14520 0.12 +2.5
1,657,213 - rfaJ HEPCGN_14520 0.22 -0.2
1,657,363 + rfaJ HEPCGN_14520 0.36 +1.5
1,657,852 + rfaJ HEPCGN_14520 0.84 +0.1
1,658,233 - HEPCGN_14525 0.14 +0.2
1,658,338 - HEPCGN_14525 0.24 -1.3
1,658,363 + HEPCGN_14525 0.27 -1.1
1,658,452 + HEPCGN_14525 0.36 -1.0
1,658,476 - HEPCGN_14525 0.38 +0.5
1,658,477 + HEPCGN_14525 0.39 -0.5
1,658,504 + HEPCGN_14525 0.41 -1.3
1,658,673 - HEPCGN_14525 0.58 -0.5
1,658,719 - HEPCGN_14525 0.63 -1.2
1,658,800 + HEPCGN_14525 0.71 -0.7
1,659,014 + -2.3

Or see this region's nucleotide sequence