Experiment: Bas55
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ytfB and acrR overlap by 17 nucleotides acrR and fabG are separated by 212 nucleotides fabG and ycjR are separated by 9 nucleotides ycjR and HEPCGN_10305 are separated by 9 nucleotides
HEPCGN_10285: ytfB - cell division protein YtfB, at 803,468 to 804,106
ytfB
HEPCGN_10290: acrR - TetR/AcrR family transcriptional regulator, at 804,090 to 804,737
acrR
HEPCGN_10295: fabG - 3-ketoacyl-ACP reductase, at 804,950 to 805,741
fabG
HEPCGN_10300: ycjR - sugar phosphate isomerase/epimerase, at 805,751 to 806,593
ycjR
HEPCGN_10305: HEPCGN_10305 - acyl-CoA hydratase, at 806,603 to 807,379
_10305
Position (kb) 804
805
806 Strain fitness (log2 ratio) -1
0
1
2 at 804.092 kb on - strand at 804.131 kb on + strand at 804.131 kb on + strand at 804.131 kb on + strand at 804.156 kb on - strand, within acrR at 804.156 kb on - strand, within acrR at 804.156 kb on - strand, within acrR at 804.216 kb on - strand, within acrR at 804.548 kb on - strand, within acrR at 804.633 kb on - strand, within acrR at 804.687 kb on + strand at 804.688 kb on - strand at 804.734 kb on - strand at 804.768 kb on + strand at 804.774 kb on - strand at 804.905 kb on + strand at 804.944 kb on + strand at 804.944 kb on + strand at 804.945 kb on - strand at 804.949 kb on + strand at 804.950 kb on - strand at 804.997 kb on + strand at 804.998 kb on - strand at 805.039 kb on + strand, within fabG at 805.294 kb on + strand, within fabG at 805.350 kb on + strand, within fabG at 805.450 kb on + strand, within fabG at 805.450 kb on + strand, within fabG at 805.451 kb on - strand, within fabG at 805.451 kb on - strand, within fabG at 805.564 kb on + strand, within fabG at 805.564 kb on + strand, within fabG at 805.675 kb on + strand at 805.686 kb on - strand at 805.723 kb on - strand at 805.723 kb on - strand at 805.726 kb on + strand at 805.727 kb on - strand at 805.765 kb on - strand at 805.786 kb on + strand at 805.811 kb on - strand at 805.831 kb on + strand at 805.832 kb on - strand at 805.934 kb on - strand, within ycjR at 805.960 kb on + strand, within ycjR at 805.968 kb on - strand, within ycjR at 805.970 kb on + strand, within ycjR at 805.971 kb on - strand, within ycjR at 806.060 kb on + strand, within ycjR at 806.062 kb on + strand, within ycjR at 806.062 kb on + strand, within ycjR at 806.063 kb on - strand, within ycjR at 806.063 kb on - strand, within ycjR at 806.236 kb on - strand, within ycjR at 806.310 kb on - strand, within ycjR at 806.585 kb on + strand at 806.684 kb on - strand, within HEPCGN_10305 at 806.687 kb on - strand, within HEPCGN_10305
Per-strain Table
Position Strand Gene LocusTag Fraction Bas55 remove 804,092 - -0.2 804,131 + -0.8 804,131 + +0.6 804,131 + +1.1 804,156 - acrR HEPCGN_10290 0.10 -0.3 804,156 - acrR HEPCGN_10290 0.10 +0.2 804,156 - acrR HEPCGN_10290 0.10 -1.2 804,216 - acrR HEPCGN_10290 0.19 -1.1 804,548 - acrR HEPCGN_10290 0.71 -0.4 804,633 - acrR HEPCGN_10290 0.84 -0.2 804,687 + -0.1 804,688 - +0.9 804,734 - +1.7 804,768 + -0.3 804,774 - -0.5 804,905 + +1.3 804,944 + +0.9 804,944 + -0.6 804,945 - -0.7 804,949 + -0.8 804,950 - +0.3 804,997 + +1.9 804,998 - +1.7 805,039 + fabG HEPCGN_10295 0.11 +0.1 805,294 + fabG HEPCGN_10295 0.43 -0.3 805,350 + fabG HEPCGN_10295 0.51 -0.5 805,450 + fabG HEPCGN_10295 0.63 -0.5 805,450 + fabG HEPCGN_10295 0.63 -0.4 805,451 - fabG HEPCGN_10295 0.63 +0.5 805,451 - fabG HEPCGN_10295 0.63 -1.4 805,564 + fabG HEPCGN_10295 0.78 -0.1 805,564 + fabG HEPCGN_10295 0.78 +0.3 805,675 + +0.1 805,686 - -1.4 805,723 - -0.6 805,723 - -1.3 805,726 + -0.0 805,727 - +0.8 805,765 - -0.7 805,786 + -0.0 805,811 - +0.5 805,831 + -0.5 805,832 - +0.5 805,934 - ycjR HEPCGN_10300 0.22 -0.9 805,960 + ycjR HEPCGN_10300 0.25 -0.6 805,968 - ycjR HEPCGN_10300 0.26 -0.9 805,970 + ycjR HEPCGN_10300 0.26 -0.4 805,971 - ycjR HEPCGN_10300 0.26 -0.5 806,060 + ycjR HEPCGN_10300 0.37 -0.4 806,062 + ycjR HEPCGN_10300 0.37 +0.2 806,062 + ycjR HEPCGN_10300 0.37 +0.1 806,063 - ycjR HEPCGN_10300 0.37 -0.7 806,063 - ycjR HEPCGN_10300 0.37 -0.9 806,236 - ycjR HEPCGN_10300 0.58 +1.9 806,310 - ycjR HEPCGN_10300 0.66 +0.2 806,585 + +1.6 806,684 - HEPCGN_10305 0.10 -0.2 806,687 - HEPCGN_10305 0.11 -1.0
Or see this region's nucleotide sequence