Experiment: Bas55
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mhpC and mhpB are separated by 17 nucleotides mhpB and mhpA are separated by 1 nucleotides mhpA and mhpR are separated by 190 nucleotides
HEPCGN_07400: mhpC - 2-hydroxy-6-oxonona-2,4-dienedioate hydrolase, at 213,774 to 214,640
mhpC
HEPCGN_07405: mhpB - 3-carboxyethylcatechol 2,3-dioxygenase, at 214,658 to 215,602
mhpB
HEPCGN_07410: mhpA - bifunctional 3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylase, at 215,604 to 217,268
mhpA
HEPCGN_07415: mhpR - DNA-binding transcriptional regulator, at 217,459 to 218,292
mhpR
Position (kb) 215
216
217
218 Strain fitness (log2 ratio) -2
-1
0
1
2 at 214.655 kb on + strand at 214.719 kb on + strand at 214.720 kb on - strand at 214.853 kb on + strand, within mhpB at 214.854 kb on - strand, within mhpB at 215.272 kb on + strand, within mhpB at 215.273 kb on - strand, within mhpB at 215.273 kb on - strand, within mhpB at 215.273 kb on - strand, within mhpB at 215.273 kb on - strand, within mhpB at 215.273 kb on - strand, within mhpB at 215.428 kb on - strand, within mhpB at 215.443 kb on - strand, within mhpB at 215.534 kb on + strand at 215.557 kb on - strand at 215.592 kb on + strand at 215.592 kb on + strand at 215.593 kb on - strand at 215.593 kb on - strand at 215.669 kb on + strand at 215.670 kb on - strand at 215.678 kb on + strand at 215.678 kb on + strand at 215.679 kb on - strand at 215.679 kb on - strand at 215.681 kb on + strand at 215.799 kb on + strand, within mhpA at 215.803 kb on - strand, within mhpA at 215.803 kb on - strand, within mhpA at 216.109 kb on + strand, within mhpA at 216.110 kb on - strand, within mhpA at 216.130 kb on + strand, within mhpA at 216.159 kb on + strand, within mhpA at 216.164 kb on + strand, within mhpA at 216.165 kb on - strand, within mhpA at 216.256 kb on + strand, within mhpA at 216.257 kb on - strand, within mhpA at 216.257 kb on - strand, within mhpA at 216.257 kb on - strand, within mhpA at 216.257 kb on - strand, within mhpA at 216.347 kb on - strand, within mhpA at 216.347 kb on - strand, within mhpA at 216.446 kb on - strand, within mhpA at 216.512 kb on + strand, within mhpA at 216.561 kb on - strand, within mhpA at 216.631 kb on + strand, within mhpA at 216.631 kb on + strand, within mhpA at 216.631 kb on + strand, within mhpA at 216.632 kb on - strand, within mhpA at 216.632 kb on - strand, within mhpA at 216.632 kb on - strand, within mhpA at 216.635 kb on + strand, within mhpA at 216.636 kb on - strand, within mhpA at 216.975 kb on - strand, within mhpA at 217.029 kb on - strand, within mhpA at 217.125 kb on - strand at 217.171 kb on + strand at 217.226 kb on + strand at 217.260 kb on + strand at 217.260 kb on + strand at 217.312 kb on + strand at 217.371 kb on - strand at 217.436 kb on + strand at 217.636 kb on + strand, within mhpR at 217.636 kb on + strand, within mhpR at 217.637 kb on - strand, within mhpR at 217.695 kb on - strand, within mhpR at 218.059 kb on + strand, within mhpR at 218.060 kb on - strand, within mhpR at 218.095 kb on - strand, within mhpR at 218.149 kb on + strand, within mhpR at 218.149 kb on + strand, within mhpR at 218.149 kb on + strand, within mhpR at 218.150 kb on - strand, within mhpR at 218.150 kb on - strand, within mhpR at 218.150 kb on - strand, within mhpR at 218.251 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas55 remove 214,655 + +2.2 214,719 + +1.4 214,720 - -1.1 214,853 + mhpB HEPCGN_07405 0.21 +1.2 214,854 - mhpB HEPCGN_07405 0.21 +0.1 215,272 + mhpB HEPCGN_07405 0.65 +0.9 215,273 - mhpB HEPCGN_07405 0.65 -0.4 215,273 - mhpB HEPCGN_07405 0.65 -0.5 215,273 - mhpB HEPCGN_07405 0.65 +0.1 215,273 - mhpB HEPCGN_07405 0.65 -0.2 215,273 - mhpB HEPCGN_07405 0.65 -0.1 215,428 - mhpB HEPCGN_07405 0.81 -0.2 215,443 - mhpB HEPCGN_07405 0.83 -0.7 215,534 + -1.0 215,557 - -1.1 215,592 + +2.1 215,592 + +0.7 215,593 - -0.1 215,593 - +0.2 215,669 + +0.9 215,670 - -0.5 215,678 + +0.5 215,678 + +0.1 215,679 - -0.3 215,679 - +0.2 215,681 + +0.1 215,799 + mhpA HEPCGN_07410 0.12 +1.2 215,803 - mhpA HEPCGN_07410 0.12 +0.2 215,803 - mhpA HEPCGN_07410 0.12 -0.6 216,109 + mhpA HEPCGN_07410 0.30 +0.7 216,110 - mhpA HEPCGN_07410 0.30 +0.3 216,130 + mhpA HEPCGN_07410 0.32 +1.1 216,159 + mhpA HEPCGN_07410 0.33 +0.4 216,164 + mhpA HEPCGN_07410 0.34 +0.1 216,165 - mhpA HEPCGN_07410 0.34 +0.0 216,256 + mhpA HEPCGN_07410 0.39 +0.4 216,257 - mhpA HEPCGN_07410 0.39 -0.1 216,257 - mhpA HEPCGN_07410 0.39 +0.4 216,257 - mhpA HEPCGN_07410 0.39 +0.1 216,257 - mhpA HEPCGN_07410 0.39 -0.1 216,347 - mhpA HEPCGN_07410 0.45 -0.2 216,347 - mhpA HEPCGN_07410 0.45 -0.7 216,446 - mhpA HEPCGN_07410 0.51 +0.1 216,512 + mhpA HEPCGN_07410 0.55 +0.1 216,561 - mhpA HEPCGN_07410 0.57 -0.3 216,631 + mhpA HEPCGN_07410 0.62 +0.9 216,631 + mhpA HEPCGN_07410 0.62 -0.4 216,631 + mhpA HEPCGN_07410 0.62 -0.8 216,632 - mhpA HEPCGN_07410 0.62 -0.7 216,632 - mhpA HEPCGN_07410 0.62 -0.1 216,632 - mhpA HEPCGN_07410 0.62 -0.2 216,635 + mhpA HEPCGN_07410 0.62 -1.3 216,636 - mhpA HEPCGN_07410 0.62 +0.3 216,975 - mhpA HEPCGN_07410 0.82 -0.6 217,029 - mhpA HEPCGN_07410 0.86 -1.3 217,125 - +0.3 217,171 + -2.2 217,226 + -1.4 217,260 + +0.2 217,260 + -0.5 217,312 + -0.2 217,371 - -0.3 217,436 + -0.7 217,636 + mhpR HEPCGN_07415 0.21 +0.6 217,636 + mhpR HEPCGN_07415 0.21 -0.2 217,637 - mhpR HEPCGN_07415 0.21 +0.7 217,695 - mhpR HEPCGN_07415 0.28 -0.5 218,059 + mhpR HEPCGN_07415 0.72 -0.7 218,060 - mhpR HEPCGN_07415 0.72 -1.3 218,095 - mhpR HEPCGN_07415 0.76 +0.7 218,149 + mhpR HEPCGN_07415 0.83 +0.4 218,149 + mhpR HEPCGN_07415 0.83 -1.4 218,149 + mhpR HEPCGN_07415 0.83 +0.2 218,150 - mhpR HEPCGN_07415 0.83 -0.2 218,150 - mhpR HEPCGN_07415 0.83 -0.0 218,150 - mhpR HEPCGN_07415 0.83 -0.3 218,251 - -0.4
Or see this region's nucleotide sequence