Experiment: Bas55
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mlc and bioD are separated by 124 nucleotides bioD and clcB overlap by 48 nucleotides clcB and HEPCGN_06130 are separated by 39 nucleotides HEPCGN_06130 and dmsD are separated by 193 nucleotides
HEPCGN_06115: mlc - DNA-binding transcriptional repressor Mlc, at 1,097,988 to 1,099,208
mlc
HEPCGN_06120: bioD - dethiobiotin synthase, at 1,099,333 to 1,100,028
bioD
HEPCGN_06125: clcB - voltage-gated ClC-type chloride channel ClcB, at 1,099,981 to 1,100,772
clcB
HEPCGN_06130: HEPCGN_06130 - Putative voltage-gated ClC-type chloride channel ClcB, at 1,100,812 to 1,101,237
_06130
HEPCGN_06135: dmsD - Tat proofreading chaperone DmsD, at 1,101,431 to 1,102,045
dmsD
Position (kb)
1099
1100
1101 Strain fitness (log2 ratio)
-2
-1
0
1 at 1099.022 kb on + strand, within mlc at 1099.087 kb on - strand at 1099.154 kb on - strand at 1099.171 kb on - strand at 1099.171 kb on - strand at 1099.266 kb on - strand at 1099.270 kb on + strand at 1099.271 kb on - strand at 1099.284 kb on + strand at 1099.360 kb on - strand at 1099.403 kb on + strand, within bioD at 1099.403 kb on + strand, within bioD at 1099.404 kb on - strand, within bioD at 1099.404 kb on - strand, within bioD at 1099.491 kb on + strand, within bioD at 1099.549 kb on - strand, within bioD at 1099.578 kb on + strand, within bioD at 1099.579 kb on - strand, within bioD at 1099.665 kb on - strand, within bioD at 1099.972 kb on + strand at 1099.983 kb on - strand at 1099.983 kb on - strand at 1099.986 kb on + strand at 1100.014 kb on - strand at 1100.088 kb on - strand, within clcB at 1100.108 kb on - strand, within clcB at 1100.231 kb on + strand, within clcB at 1100.231 kb on + strand, within clcB at 1100.232 kb on - strand, within clcB at 1100.232 kb on - strand, within clcB at 1100.272 kb on - strand, within clcB at 1100.383 kb on + strand, within clcB at 1100.392 kb on - strand, within clcB at 1100.394 kb on - strand, within clcB at 1100.489 kb on - strand, within clcB at 1100.522 kb on - strand, within clcB at 1100.559 kb on + strand, within clcB at 1100.560 kb on - strand, within clcB at 1100.576 kb on + strand, within clcB at 1100.608 kb on + strand, within clcB at 1100.609 kb on - strand, within clcB at 1100.609 kb on - strand, within clcB at 1100.615 kb on + strand, within clcB at 1100.735 kb on - strand at 1100.735 kb on - strand at 1100.860 kb on + strand, within HEPCGN_06130 at 1100.908 kb on + strand, within HEPCGN_06130 at 1100.985 kb on - strand, within HEPCGN_06130 at 1100.985 kb on - strand, within HEPCGN_06130 at 1101.237 kb on + strand at 1101.237 kb on + strand at 1101.255 kb on - strand at 1101.262 kb on + strand at 1101.262 kb on + strand at 1101.262 kb on + strand at 1101.263 kb on - strand at 1101.263 kb on - strand at 1101.419 kb on - strand at 1101.494 kb on + strand, within dmsD
Per-strain Table
Position Strand Gene LocusTag Fraction Bas55 remove 1,099,022 + mlc HEPCGN_06115 0.85 -1.7 1,099,087 - -0.3 1,099,154 - -0.1 1,099,171 - +0.7 1,099,171 - +0.7 1,099,266 - -1.1 1,099,270 + -0.4 1,099,271 - -0.6 1,099,284 + +0.7 1,099,360 - -0.4 1,099,403 + bioD HEPCGN_06120 0.10 -0.1 1,099,403 + bioD HEPCGN_06120 0.10 +0.1 1,099,404 - bioD HEPCGN_06120 0.10 -0.1 1,099,404 - bioD HEPCGN_06120 0.10 -1.0 1,099,491 + bioD HEPCGN_06120 0.23 +1.3 1,099,549 - bioD HEPCGN_06120 0.31 +0.1 1,099,578 + bioD HEPCGN_06120 0.35 -0.8 1,099,579 - bioD HEPCGN_06120 0.35 +0.8 1,099,665 - bioD HEPCGN_06120 0.48 +1.7 1,099,972 + +0.4 1,099,983 - +1.7 1,099,983 - +1.4 1,099,986 + +0.6 1,100,014 - -0.8 1,100,088 - clcB HEPCGN_06125 0.14 +0.1 1,100,108 - clcB HEPCGN_06125 0.16 +0.7 1,100,231 + clcB HEPCGN_06125 0.32 +0.0 1,100,231 + clcB HEPCGN_06125 0.32 -0.0 1,100,232 - clcB HEPCGN_06125 0.32 +0.5 1,100,232 - clcB HEPCGN_06125 0.32 -0.3 1,100,272 - clcB HEPCGN_06125 0.37 +0.8 1,100,383 + clcB HEPCGN_06125 0.51 -0.1 1,100,392 - clcB HEPCGN_06125 0.52 -1.9 1,100,394 - clcB HEPCGN_06125 0.52 +0.4 1,100,489 - clcB HEPCGN_06125 0.64 +0.5 1,100,522 - clcB HEPCGN_06125 0.68 +1.1 1,100,559 + clcB HEPCGN_06125 0.73 +0.1 1,100,560 - clcB HEPCGN_06125 0.73 -0.5 1,100,576 + clcB HEPCGN_06125 0.75 +0.1 1,100,608 + clcB HEPCGN_06125 0.79 -0.4 1,100,609 - clcB HEPCGN_06125 0.79 -0.9 1,100,609 - clcB HEPCGN_06125 0.79 +1.3 1,100,615 + clcB HEPCGN_06125 0.80 +1.7 1,100,735 - -0.0 1,100,735 - +0.3 1,100,860 + HEPCGN_06130 0.11 +0.2 1,100,908 + HEPCGN_06130 0.23 -1.1 1,100,985 - HEPCGN_06130 0.41 +1.3 1,100,985 - HEPCGN_06130 0.41 +0.5 1,101,237 + +1.1 1,101,237 + +1.1 1,101,255 - -0.1 1,101,262 + -0.4 1,101,262 + -0.6 1,101,262 + -1.2 1,101,263 - -0.1 1,101,263 - -0.8 1,101,419 - +1.0 1,101,494 + dmsD HEPCGN_06135 0.10 +0.7
Or see this region's nucleotide sequence