Experiment: Bas55
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ruvB and znuB are separated by 147 nucleotides znuB and znuC overlap by 4 nucleotides znuC and znuA are separated by 24 nucleotides
HEPCGN_04695: ruvB - Holliday junction branch migration DNA helicase RuvB, at 817,452 to 818,462
ruvB
HEPCGN_04700: znuB - zinc ABC transporter permease subunit ZnuB, at 818,610 to 819,395
znuB
HEPCGN_04705: znuC - zinc ABC transporter ATP-binding protein ZnuC, at 819,392 to 820,147
znuC
HEPCGN_04710: znuA - zinc ABC transporter substrate-binding protein ZnuA, at 820,172 to 821,158
znuA
Position (kb) 818
819
820 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 818.022 kb on + strand, within ruvB at 818.024 kb on + strand, within ruvB at 818.461 kb on - strand at 818.461 kb on - strand at 818.531 kb on - strand at 818.558 kb on + strand at 818.558 kb on + strand at 818.612 kb on - strand at 818.793 kb on - strand, within znuB at 819.009 kb on - strand, within znuB at 819.056 kb on - strand, within znuB at 819.093 kb on - strand, within znuB at 819.141 kb on + strand, within znuB at 819.180 kb on + strand, within znuB at 819.224 kb on - strand, within znuB at 819.287 kb on + strand, within znuB at 819.299 kb on - strand, within znuB at 819.306 kb on - strand, within znuB at 819.452 kb on - strand at 819.465 kb on + strand at 819.466 kb on - strand at 819.469 kb on + strand, within znuC at 819.503 kb on - strand, within znuC at 819.624 kb on - strand, within znuC at 819.662 kb on - strand, within znuC at 819.672 kb on - strand, within znuC at 819.848 kb on - strand, within znuC at 819.856 kb on + strand, within znuC at 819.857 kb on - strand, within znuC at 819.933 kb on - strand, within znuC at 819.933 kb on - strand, within znuC at 819.948 kb on - strand, within znuC at 820.021 kb on + strand, within znuC at 820.181 kb on + strand at 820.208 kb on - strand at 820.380 kb on + strand, within znuA
Per-strain Table
Position Strand Gene LocusTag Fraction Bas55 remove 818,022 + ruvB HEPCGN_04695 0.56 +0.6 818,024 + ruvB HEPCGN_04695 0.57 -1.6 818,461 - -0.4 818,461 - -0.2 818,531 - +0.2 818,558 + +1.1 818,558 + +1.1 818,612 - -0.0 818,793 - znuB HEPCGN_04700 0.23 +0.3 819,009 - znuB HEPCGN_04700 0.51 +2.1 819,056 - znuB HEPCGN_04700 0.57 +0.4 819,093 - znuB HEPCGN_04700 0.61 +0.3 819,141 + znuB HEPCGN_04700 0.68 -0.4 819,180 + znuB HEPCGN_04700 0.73 +0.8 819,224 - znuB HEPCGN_04700 0.78 -0.8 819,287 + znuB HEPCGN_04700 0.86 +1.8 819,299 - znuB HEPCGN_04700 0.88 +0.5 819,306 - znuB HEPCGN_04700 0.89 +1.2 819,452 - -0.4 819,465 + -1.0 819,466 - -0.1 819,469 + znuC HEPCGN_04705 0.10 +0.5 819,503 - znuC HEPCGN_04705 0.15 -0.4 819,624 - znuC HEPCGN_04705 0.31 -1.9 819,662 - znuC HEPCGN_04705 0.36 -0.9 819,672 - znuC HEPCGN_04705 0.37 +0.0 819,848 - znuC HEPCGN_04705 0.60 +1.2 819,856 + znuC HEPCGN_04705 0.61 -0.1 819,857 - znuC HEPCGN_04705 0.62 +0.6 819,933 - znuC HEPCGN_04705 0.72 -0.2 819,933 - znuC HEPCGN_04705 0.72 -0.6 819,948 - znuC HEPCGN_04705 0.74 -0.8 820,021 + znuC HEPCGN_04705 0.83 +3.4 820,181 + -1.0 820,208 - -0.2 820,380 + znuA HEPCGN_04710 0.21 -0.5
Or see this region's nucleotide sequence