Experiment: Bas19
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dsbD and yjdC are separated by 36 nucleotides yjdC and Phe_trna are separated by 106 nucleotides Phe_trna and HEPCGN_10690 are separated by 197 nucleotides HEPCGN_10690 and HEPCGN_10695 are separated by 361 nucleotides HEPCGN_10695 and HEPCGN_10700 are separated by 336 nucleotides
HEPCGN_10675: dsbD - protein-disulfide reductase DsbD, at 874,146 to 875,843
dsbD
HEPCGN_10680: yjdC - transcriptional regulator, at 875,880 to 876,455
yjdC
HEPCGN_10685: Phe_trna - tRNA-Phe(gaa), at 876,562 to 876,637
_trna
HEPCGN_10690: HEPCGN_10690 - integrase, at 876,835 to 878,097
_10690
HEPCGN_10695: HEPCGN_10695 - peptide ABC transporter substrate-binding protein, at 878,459 to 878,623
_10695
HEPCGN_10700: HEPCGN_10700 - lactate permease, at 878,960 to 880,516
_10700
Position (kb) 876
877
878
879 Strain fitness (log2 ratio) -2
-1
0
1 at 875.896 kb on + strand at 875.896 kb on + strand at 875.897 kb on - strand at 875.993 kb on - strand, within yjdC at 876.027 kb on + strand, within yjdC at 876.058 kb on + strand, within yjdC at 876.058 kb on + strand, within yjdC at 876.058 kb on + strand, within yjdC at 876.059 kb on - strand, within yjdC at 876.127 kb on - strand, within yjdC at 876.410 kb on + strand at 876.931 kb on - strand at 876.931 kb on - strand at 877.116 kb on - strand, within HEPCGN_10690 at 877.188 kb on + strand, within HEPCGN_10690 at 877.533 kb on - strand, within HEPCGN_10690 at 877.725 kb on - strand, within HEPCGN_10690 at 877.779 kb on + strand, within HEPCGN_10690 at 877.802 kb on + strand, within HEPCGN_10690 at 877.955 kb on + strand, within HEPCGN_10690 at 877.966 kb on + strand, within HEPCGN_10690 at 877.974 kb on + strand at 877.975 kb on - strand at 878.010 kb on - strand at 878.053 kb on - strand at 878.297 kb on + strand at 878.400 kb on + strand at 878.454 kb on + strand at 878.454 kb on + strand at 878.499 kb on - strand, within HEPCGN_10695 at 878.516 kb on + strand, within HEPCGN_10695 at 878.516 kb on + strand, within HEPCGN_10695 at 878.519 kb on + strand, within HEPCGN_10695 at 878.519 kb on + strand, within HEPCGN_10695 at 878.569 kb on + strand, within HEPCGN_10695 at 878.592 kb on - strand, within HEPCGN_10695 at 878.597 kb on + strand, within HEPCGN_10695 at 878.597 kb on + strand, within HEPCGN_10695 at 878.602 kb on + strand, within HEPCGN_10695 at 878.602 kb on + strand, within HEPCGN_10695 at 878.615 kb on - strand at 878.697 kb on + strand at 878.878 kb on - strand at 878.993 kb on - strand at 878.993 kb on - strand at 879.047 kb on + strand at 879.047 kb on + strand at 879.047 kb on + strand at 879.047 kb on + strand at 879.048 kb on - strand at 879.048 kb on - strand at 879.058 kb on + strand at 879.058 kb on + strand at 879.078 kb on + strand at 879.079 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas19 remove 875,896 + -0.2 875,896 + +0.1 875,897 - +1.2 875,993 - yjdC HEPCGN_10680 0.20 -0.2 876,027 + yjdC HEPCGN_10680 0.26 +0.9 876,058 + yjdC HEPCGN_10680 0.31 -0.6 876,058 + yjdC HEPCGN_10680 0.31 -0.7 876,058 + yjdC HEPCGN_10680 0.31 +1.3 876,059 - yjdC HEPCGN_10680 0.31 -0.1 876,127 - yjdC HEPCGN_10680 0.43 -0.4 876,410 + +0.0 876,931 - +0.0 876,931 - +0.1 877,116 - HEPCGN_10690 0.22 +0.0 877,188 + HEPCGN_10690 0.28 -0.3 877,533 - HEPCGN_10690 0.55 -0.3 877,725 - HEPCGN_10690 0.70 -1.8 877,779 + HEPCGN_10690 0.75 -0.7 877,802 + HEPCGN_10690 0.77 -1.5 877,955 + HEPCGN_10690 0.89 +0.5 877,966 + HEPCGN_10690 0.90 +0.6 877,974 + +0.2 877,975 - +0.1 878,010 - +0.5 878,053 - -0.0 878,297 + +0.7 878,400 + -1.2 878,454 + +0.1 878,454 + -1.2 878,499 - HEPCGN_10695 0.24 +0.1 878,516 + HEPCGN_10695 0.35 -0.2 878,516 + HEPCGN_10695 0.35 +0.7 878,519 + HEPCGN_10695 0.36 -1.1 878,519 + HEPCGN_10695 0.36 +0.1 878,569 + HEPCGN_10695 0.67 +0.7 878,592 - HEPCGN_10695 0.81 +0.2 878,597 + HEPCGN_10695 0.84 -0.3 878,597 + HEPCGN_10695 0.84 +0.2 878,602 + HEPCGN_10695 0.87 -0.4 878,602 + HEPCGN_10695 0.87 +0.2 878,615 - +1.5 878,697 + +0.2 878,878 - +0.1 878,993 - +1.0 878,993 - -0.8 879,047 + -0.8 879,047 + -0.1 879,047 + +1.4 879,047 + -0.3 879,048 - -0.4 879,048 - +0.1 879,058 + +1.5 879,058 + -0.1 879,078 + -1.7 879,079 - -0.4
Or see this region's nucleotide sequence