Experiment: Bas19
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yfcC and HEPCGN_10055 are separated by 115 nucleotides HEPCGN_10055 and pyrB are separated by 227 nucleotides pyrB and pyrI are separated by 12 nucleotides pyrI and ridA are separated by 72 nucleotides
HEPCGN_10050: yfcC - C4-dicarboxylate ABC transporter, at 755,542 to 756,945
yfcC
HEPCGN_10055: HEPCGN_10055 - ArgR family transcriptional regulator, at 757,061 to 757,549
_10055
HEPCGN_10060: pyrB - aspartate carbamoyltransferase, at 757,777 to 758,712
pyrB
HEPCGN_10065: pyrI - aspartate carbamoyltransferase regulatory subunit, at 758,725 to 759,186
pyrI
HEPCGN_10070: ridA - 2-iminobutanoate/2-iminopropanoate deaminase, at 759,259 to 759,645
ridA
Position (kb) 757
758
759 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 756.796 kb on - strand, within yfcC at 756.796 kb on - strand, within yfcC at 756.913 kb on - strand at 757.013 kb on - strand at 757.038 kb on + strand at 757.044 kb on + strand at 757.048 kb on + strand at 757.060 kb on + strand at 757.140 kb on - strand, within HEPCGN_10055 at 757.252 kb on + strand, within HEPCGN_10055 at 757.349 kb on - strand, within HEPCGN_10055 at 757.493 kb on - strand, within HEPCGN_10055 at 757.570 kb on + strand at 757.638 kb on + strand at 757.638 kb on + strand at 757.638 kb on + strand at 757.638 kb on + strand at 757.639 kb on - strand at 757.639 kb on - strand at 757.639 kb on - strand at 757.791 kb on - strand at 757.804 kb on - strand at 757.840 kb on - strand at 757.840 kb on - strand at 757.909 kb on - strand, within pyrB at 757.909 kb on - strand, within pyrB at 758.013 kb on + strand, within pyrB at 758.013 kb on + strand, within pyrB at 758.204 kb on + strand, within pyrB at 758.332 kb on + strand, within pyrB at 758.333 kb on - strand, within pyrB at 758.369 kb on - strand, within pyrB at 758.380 kb on - strand, within pyrB at 758.421 kb on + strand, within pyrB at 758.421 kb on + strand, within pyrB at 758.422 kb on - strand, within pyrB at 758.422 kb on - strand, within pyrB at 758.445 kb on - strand, within pyrB at 758.455 kb on + strand, within pyrB at 758.720 kb on + strand at 758.720 kb on + strand at 758.720 kb on + strand at 758.720 kb on + strand at 758.721 kb on - strand at 758.757 kb on + strand at 758.774 kb on + strand, within pyrI at 758.806 kb on - strand, within pyrI at 758.953 kb on + strand, within pyrI at 758.953 kb on + strand, within pyrI at 758.989 kb on + strand, within pyrI at 758.990 kb on - strand, within pyrI at 759.066 kb on + strand, within pyrI at 759.067 kb on - strand, within pyrI at 759.142 kb on + strand at 759.142 kb on + strand at 759.165 kb on + strand at 759.200 kb on + strand at 759.200 kb on + strand at 759.201 kb on - strand at 759.243 kb on + strand at 759.244 kb on - strand at 759.297 kb on + strand at 759.307 kb on + strand, within ridA at 759.311 kb on + strand, within ridA at 759.312 kb on - strand, within ridA at 759.312 kb on - strand, within ridA at 759.312 kb on - strand, within ridA at 759.429 kb on - strand, within ridA at 759.529 kb on + strand, within ridA at 759.529 kb on + strand, within ridA at 759.665 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas19 remove 756,796 - yfcC HEPCGN_10050 0.89 -0.1 756,796 - yfcC HEPCGN_10050 0.89 -0.1 756,913 - -0.3 757,013 - +1.5 757,038 + +2.9 757,044 + +3.6 757,048 + +2.6 757,060 + +0.3 757,140 - HEPCGN_10055 0.16 -1.0 757,252 + HEPCGN_10055 0.39 -0.4 757,349 - HEPCGN_10055 0.59 -0.1 757,493 - HEPCGN_10055 0.88 +0.0 757,570 + -0.2 757,638 + -0.3 757,638 + -1.1 757,638 + -0.8 757,638 + -0.2 757,639 - +0.9 757,639 - -1.1 757,639 - -0.0 757,791 - +0.2 757,804 - +1.8 757,840 - -0.3 757,840 - -0.1 757,909 - pyrB HEPCGN_10060 0.14 -0.2 757,909 - pyrB HEPCGN_10060 0.14 +0.2 758,013 + pyrB HEPCGN_10060 0.25 -0.6 758,013 + pyrB HEPCGN_10060 0.25 +1.0 758,204 + pyrB HEPCGN_10060 0.46 +0.1 758,332 + pyrB HEPCGN_10060 0.59 +0.9 758,333 - pyrB HEPCGN_10060 0.59 +0.8 758,369 - pyrB HEPCGN_10060 0.63 +0.4 758,380 - pyrB HEPCGN_10060 0.64 -0.2 758,421 + pyrB HEPCGN_10060 0.69 +0.5 758,421 + pyrB HEPCGN_10060 0.69 -0.9 758,422 - pyrB HEPCGN_10060 0.69 -0.6 758,422 - pyrB HEPCGN_10060 0.69 -1.2 758,445 - pyrB HEPCGN_10060 0.71 -1.0 758,455 + pyrB HEPCGN_10060 0.72 +1.7 758,720 + +0.4 758,720 + -0.3 758,720 + +0.3 758,720 + -0.0 758,721 - -0.6 758,757 + +0.1 758,774 + pyrI HEPCGN_10065 0.11 -0.0 758,806 - pyrI HEPCGN_10065 0.18 -0.2 758,953 + pyrI HEPCGN_10065 0.49 +0.3 758,953 + pyrI HEPCGN_10065 0.49 +0.3 758,989 + pyrI HEPCGN_10065 0.57 -1.1 758,990 - pyrI HEPCGN_10065 0.57 -0.8 759,066 + pyrI HEPCGN_10065 0.74 -0.5 759,067 - pyrI HEPCGN_10065 0.74 +0.1 759,142 + +0.2 759,142 + +1.6 759,165 + +0.6 759,200 + -0.2 759,200 + +0.6 759,201 - -1.6 759,243 + +0.4 759,244 - +0.3 759,297 + -1.9 759,307 + ridA HEPCGN_10070 0.12 -1.1 759,311 + ridA HEPCGN_10070 0.13 -0.7 759,312 - ridA HEPCGN_10070 0.14 -0.5 759,312 - ridA HEPCGN_10070 0.14 -0.2 759,312 - ridA HEPCGN_10070 0.14 -0.8 759,429 - ridA HEPCGN_10070 0.44 +0.4 759,529 + ridA HEPCGN_10070 0.70 +0.8 759,529 + ridA HEPCGN_10070 0.70 +0.4 759,665 + -1.4
Or see this region's nucleotide sequence