Experiment: Bas19
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cueO and yacC are separated by 204 nucleotides yacC and speE are separated by 105 nucleotides speE and speD are separated by 15 nucleotides speD and yacL are separated by 37 nucleotides
HEPCGN_08435: cueO - multicopper oxidase CueO, at 424,095 to 425,645
cueO
HEPCGN_08440: yacC - YacC family pilotin-like protein, at 425,850 to 426,158
yacC
HEPCGN_08445: speE - polyamine aminopropyltransferase, at 426,264 to 427,130
speE
HEPCGN_08450: speD - adenosylmethionine decarboxylase, at 427,146 to 427,940
speD
HEPCGN_08455: yacL - protein YacL, at 427,978 to 428,340
yacL
Position (kb) 426
427
428 Strain fitness (log2 ratio) -2
-1
0
1 at 425.374 kb on + strand, within cueO at 425.374 kb on + strand, within cueO at 425.407 kb on - strand, within cueO at 425.450 kb on + strand, within cueO at 425.493 kb on - strand at 425.496 kb on + strand at 425.497 kb on - strand at 425.646 kb on - strand at 425.646 kb on - strand at 425.657 kb on + strand at 425.696 kb on - strand at 425.737 kb on + strand at 425.792 kb on + strand at 425.900 kb on + strand, within yacC at 426.027 kb on + strand, within yacC at 426.027 kb on + strand, within yacC at 426.028 kb on - strand, within yacC at 426.209 kb on + strand at 426.209 kb on + strand at 426.210 kb on - strand at 426.210 kb on - strand at 426.213 kb on + strand at 426.214 kb on - strand at 426.216 kb on + strand at 426.217 kb on - strand at 426.296 kb on - strand at 426.296 kb on - strand at 426.330 kb on - strand at 426.340 kb on - strand at 426.422 kb on - strand, within speE at 426.446 kb on + strand, within speE at 426.622 kb on - strand, within speE at 426.625 kb on + strand, within speE at 426.649 kb on - strand, within speE at 426.854 kb on - strand, within speE at 426.939 kb on + strand, within speE at 426.939 kb on + strand, within speE at 426.977 kb on + strand, within speE at 426.989 kb on - strand, within speE at 427.053 kb on + strand at 427.054 kb on - strand at 427.207 kb on - strand at 427.250 kb on + strand, within speD at 427.251 kb on - strand, within speD at 427.251 kb on - strand, within speD at 427.251 kb on - strand, within speD at 427.251 kb on - strand, within speD at 427.257 kb on + strand, within speD at 427.258 kb on - strand, within speD at 427.258 kb on - strand, within speD at 427.272 kb on + strand, within speD at 427.317 kb on - strand, within speD at 427.331 kb on + strand, within speD at 427.484 kb on + strand, within speD at 427.492 kb on + strand, within speD at 427.496 kb on + strand, within speD at 427.496 kb on + strand, within speD at 427.496 kb on + strand, within speD at 427.497 kb on - strand, within speD at 427.497 kb on - strand, within speD at 427.497 kb on - strand, within speD at 427.497 kb on - strand, within speD at 427.497 kb on - strand, within speD at 427.528 kb on + strand, within speD at 427.528 kb on + strand, within speD at 427.529 kb on - strand, within speD at 427.529 kb on - strand, within speD at 427.529 kb on - strand, within speD at 427.532 kb on + strand, within speD at 427.596 kb on + strand, within speD at 427.628 kb on - strand, within speD at 427.673 kb on + strand, within speD at 427.700 kb on + strand, within speD at 427.701 kb on - strand, within speD at 427.719 kb on - strand, within speD at 427.818 kb on - strand, within speD at 427.824 kb on + strand, within speD at 427.824 kb on + strand, within speD at 427.825 kb on - strand, within speD at 427.825 kb on - strand, within speD at 427.912 kb on - strand at 427.915 kb on - strand at 427.926 kb on - strand at 427.979 kb on + strand at 428.064 kb on - strand, within yacL at 428.066 kb on + strand, within yacL at 428.066 kb on + strand, within yacL at 428.066 kb on + strand, within yacL at 428.066 kb on + strand, within yacL at 428.066 kb on + strand, within yacL at 428.067 kb on - strand, within yacL
Per-strain Table
Position Strand Gene LocusTag Fraction Bas19 remove 425,374 + cueO HEPCGN_08435 0.82 +0.3 425,374 + cueO HEPCGN_08435 0.82 +1.4 425,407 - cueO HEPCGN_08435 0.85 +0.4 425,450 + cueO HEPCGN_08435 0.87 +0.3 425,493 - -0.1 425,496 + -0.5 425,497 - -0.5 425,646 - +1.3 425,646 - -0.7 425,657 + -0.7 425,696 - +0.5 425,737 + +0.6 425,792 + -0.1 425,900 + yacC HEPCGN_08440 0.16 -1.0 426,027 + yacC HEPCGN_08440 0.57 -0.4 426,027 + yacC HEPCGN_08440 0.57 -2.1 426,028 - yacC HEPCGN_08440 0.58 -0.9 426,209 + +0.1 426,209 + -0.0 426,210 - +0.9 426,210 - +1.1 426,213 + -0.3 426,214 - +0.3 426,216 + +0.3 426,217 - -0.0 426,296 - -0.1 426,296 - +0.5 426,330 - -1.1 426,340 - +0.9 426,422 - speE HEPCGN_08445 0.18 +0.1 426,446 + speE HEPCGN_08445 0.21 -0.3 426,622 - speE HEPCGN_08445 0.41 +1.6 426,625 + speE HEPCGN_08445 0.42 -0.5 426,649 - speE HEPCGN_08445 0.44 +1.0 426,854 - speE HEPCGN_08445 0.68 +1.2 426,939 + speE HEPCGN_08445 0.78 -1.0 426,939 + speE HEPCGN_08445 0.78 -1.0 426,977 + speE HEPCGN_08445 0.82 -0.3 426,989 - speE HEPCGN_08445 0.84 -1.0 427,053 + -1.3 427,054 - -0.2 427,207 - +0.6 427,250 + speD HEPCGN_08450 0.13 -0.2 427,251 - speD HEPCGN_08450 0.13 +1.4 427,251 - speD HEPCGN_08450 0.13 +1.2 427,251 - speD HEPCGN_08450 0.13 -0.0 427,251 - speD HEPCGN_08450 0.13 -0.1 427,257 + speD HEPCGN_08450 0.14 +0.1 427,258 - speD HEPCGN_08450 0.14 +0.2 427,258 - speD HEPCGN_08450 0.14 -0.0 427,272 + speD HEPCGN_08450 0.16 -0.6 427,317 - speD HEPCGN_08450 0.22 -0.8 427,331 + speD HEPCGN_08450 0.23 -0.8 427,484 + speD HEPCGN_08450 0.43 -0.5 427,492 + speD HEPCGN_08450 0.44 +0.2 427,496 + speD HEPCGN_08450 0.44 -0.7 427,496 + speD HEPCGN_08450 0.44 +0.6 427,496 + speD HEPCGN_08450 0.44 -0.5 427,497 - speD HEPCGN_08450 0.44 -0.1 427,497 - speD HEPCGN_08450 0.44 -0.2 427,497 - speD HEPCGN_08450 0.44 +0.2 427,497 - speD HEPCGN_08450 0.44 +0.6 427,497 - speD HEPCGN_08450 0.44 -0.3 427,528 + speD HEPCGN_08450 0.48 +0.4 427,528 + speD HEPCGN_08450 0.48 -0.1 427,529 - speD HEPCGN_08450 0.48 -0.4 427,529 - speD HEPCGN_08450 0.48 +0.3 427,529 - speD HEPCGN_08450 0.48 +0.1 427,532 + speD HEPCGN_08450 0.49 +0.5 427,596 + speD HEPCGN_08450 0.57 -0.0 427,628 - speD HEPCGN_08450 0.61 +0.4 427,673 + speD HEPCGN_08450 0.66 +0.8 427,700 + speD HEPCGN_08450 0.70 +0.4 427,701 - speD HEPCGN_08450 0.70 +0.9 427,719 - speD HEPCGN_08450 0.72 +0.9 427,818 - speD HEPCGN_08450 0.85 -0.4 427,824 + speD HEPCGN_08450 0.85 -0.2 427,824 + speD HEPCGN_08450 0.85 +0.6 427,825 - speD HEPCGN_08450 0.85 +0.3 427,825 - speD HEPCGN_08450 0.85 +1.1 427,912 - +0.6 427,915 - -0.0 427,926 - +0.0 427,979 + -1.8 428,064 - yacL HEPCGN_08455 0.24 -0.8 428,066 + yacL HEPCGN_08455 0.24 +1.1 428,066 + yacL HEPCGN_08455 0.24 +0.2 428,066 + yacL HEPCGN_08455 0.24 -1.3 428,066 + yacL HEPCGN_08455 0.24 +0.7 428,066 + yacL HEPCGN_08455 0.24 +0.8 428,067 - yacL HEPCGN_08455 0.25 -0.2
Or see this region's nucleotide sequence