Experiment: Bas19
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rpnC and panD are separated by 273 nucleotides panD and yadE are separated by 3 nucleotides yadE and yadI are separated by 63 nucleotides yadI and HEPCGN_08410 are separated by 104 nucleotides
HEPCGN_08390: rpnC - Recombination-promoting nuclease RpnC, at 414,983 to 415,915
rpnC
HEPCGN_08395: panD - aspartate 1-decarboxylase, at 416,189 to 416,569
panD
HEPCGN_08400: yadE - Uncharacterized protein YadE, at 416,573 to 417,802
yadE
HEPCGN_08405: yadI - Putative phosphotransferase enzyme IIA component YadI, at 417,866 to 418,306
yadI
HEPCGN_08410: HEPCGN_08410 - Inner membrane transport permease YadH, at 418,411 to 419,178
_08410
Position (kb) 416
417
418 Strain fitness (log2 ratio) -2
-1
0
1
2 at 415.582 kb on + strand, within rpnC at 415.621 kb on + strand, within rpnC at 415.692 kb on + strand, within rpnC at 415.811 kb on + strand, within rpnC at 415.866 kb on - strand at 416.095 kb on - strand at 416.174 kb on - strand at 416.181 kb on + strand at 416.182 kb on - strand at 416.252 kb on + strand, within panD at 416.253 kb on - strand, within panD at 416.253 kb on - strand, within panD at 416.324 kb on - strand, within panD at 416.360 kb on + strand, within panD at 416.361 kb on - strand, within panD at 416.361 kb on - strand, within panD at 416.392 kb on + strand, within panD at 416.392 kb on + strand, within panD at 416.465 kb on - strand, within panD at 416.485 kb on + strand, within panD at 416.486 kb on - strand, within panD at 416.508 kb on - strand, within panD at 416.574 kb on + strand at 416.590 kb on + strand at 416.630 kb on + strand at 416.631 kb on - strand at 416.631 kb on - strand at 416.636 kb on - strand at 416.638 kb on - strand at 416.648 kb on + strand at 416.648 kb on + strand at 416.649 kb on - strand at 416.675 kb on + strand at 416.676 kb on - strand at 416.746 kb on + strand, within yadE at 416.846 kb on - strand, within yadE at 416.859 kb on - strand, within yadE at 416.918 kb on + strand, within yadE at 417.013 kb on + strand, within yadE at 417.031 kb on + strand, within yadE at 417.036 kb on + strand, within yadE at 417.127 kb on + strand, within yadE at 417.138 kb on + strand, within yadE at 417.236 kb on - strand, within yadE at 417.236 kb on - strand, within yadE at 417.279 kb on + strand, within yadE at 417.280 kb on - strand, within yadE at 417.280 kb on - strand, within yadE at 417.280 kb on - strand, within yadE at 417.331 kb on - strand, within yadE at 417.331 kb on - strand, within yadE at 417.488 kb on + strand, within yadE at 417.488 kb on + strand, within yadE at 417.489 kb on - strand, within yadE at 417.590 kb on - strand, within yadE at 417.727 kb on + strand at 417.727 kb on + strand at 417.727 kb on + strand at 417.728 kb on - strand at 417.730 kb on - strand at 417.785 kb on - strand at 417.798 kb on + strand at 417.834 kb on + strand at 417.839 kb on + strand at 417.840 kb on - strand at 417.876 kb on + strand at 417.877 kb on - strand at 417.883 kb on + strand at 417.931 kb on + strand, within yadI at 417.962 kb on - strand, within yadI at 417.962 kb on - strand, within yadI at 418.073 kb on + strand, within yadI at 418.073 kb on + strand, within yadI at 418.106 kb on + strand, within yadI at 418.181 kb on - strand, within yadI at 418.183 kb on + strand, within yadI at 418.184 kb on - strand, within yadI at 418.184 kb on - strand, within yadI at 418.190 kb on + strand, within yadI at 418.260 kb on - strand, within yadI at 418.288 kb on + strand at 418.289 kb on - strand at 418.289 kb on - strand at 418.301 kb on + strand at 418.301 kb on + strand at 418.302 kb on - strand at 418.320 kb on - strand at 418.335 kb on + strand at 418.335 kb on + strand at 418.353 kb on + strand at 418.363 kb on + strand at 418.407 kb on - strand at 418.412 kb on + strand at 418.413 kb on - strand at 418.413 kb on - strand at 418.416 kb on + strand at 418.460 kb on + strand at 418.461 kb on - strand at 418.474 kb on - strand at 418.474 kb on - strand at 418.486 kb on + strand at 418.498 kb on - strand, within HEPCGN_08410 at 418.498 kb on - strand, within HEPCGN_08410 at 418.554 kb on + strand, within HEPCGN_08410 at 418.555 kb on - strand, within HEPCGN_08410 at 418.557 kb on - strand, within HEPCGN_08410 at 418.635 kb on - strand, within HEPCGN_08410 at 418.762 kb on + strand, within HEPCGN_08410
Per-strain Table
Position Strand Gene LocusTag Fraction Bas19 remove 415,582 + rpnC HEPCGN_08390 0.64 -0.2 415,621 + rpnC HEPCGN_08390 0.68 +0.6 415,692 + rpnC HEPCGN_08390 0.76 +0.6 415,811 + rpnC HEPCGN_08390 0.89 +0.6 415,866 - -1.8 416,095 - -0.3 416,174 - +0.3 416,181 + +0.3 416,182 - -0.3 416,252 + panD HEPCGN_08395 0.17 -0.1 416,253 - panD HEPCGN_08395 0.17 +0.3 416,253 - panD HEPCGN_08395 0.17 +0.2 416,324 - panD HEPCGN_08395 0.35 -0.6 416,360 + panD HEPCGN_08395 0.45 +0.0 416,361 - panD HEPCGN_08395 0.45 -0.3 416,361 - panD HEPCGN_08395 0.45 +1.1 416,392 + panD HEPCGN_08395 0.53 +0.3 416,392 + panD HEPCGN_08395 0.53 +0.1 416,465 - panD HEPCGN_08395 0.72 -1.5 416,485 + panD HEPCGN_08395 0.78 -1.3 416,486 - panD HEPCGN_08395 0.78 -0.0 416,508 - panD HEPCGN_08395 0.84 -1.0 416,574 + +0.9 416,590 + +0.4 416,630 + +0.2 416,631 - -0.2 416,631 - +0.2 416,636 - -0.3 416,638 - -1.1 416,648 + -0.1 416,648 + -0.4 416,649 - -0.3 416,675 + -0.0 416,676 - +0.4 416,746 + yadE HEPCGN_08400 0.14 +0.2 416,846 - yadE HEPCGN_08400 0.22 -0.7 416,859 - yadE HEPCGN_08400 0.23 +0.4 416,918 + yadE HEPCGN_08400 0.28 +0.6 417,013 + yadE HEPCGN_08400 0.36 +0.6 417,031 + yadE HEPCGN_08400 0.37 -0.4 417,036 + yadE HEPCGN_08400 0.38 -0.5 417,127 + yadE HEPCGN_08400 0.45 -0.1 417,138 + yadE HEPCGN_08400 0.46 -1.0 417,236 - yadE HEPCGN_08400 0.54 -0.0 417,236 - yadE HEPCGN_08400 0.54 -0.8 417,279 + yadE HEPCGN_08400 0.57 -1.1 417,280 - yadE HEPCGN_08400 0.57 -0.4 417,280 - yadE HEPCGN_08400 0.57 -0.1 417,280 - yadE HEPCGN_08400 0.57 -1.1 417,331 - yadE HEPCGN_08400 0.62 -0.5 417,331 - yadE HEPCGN_08400 0.62 +0.4 417,488 + yadE HEPCGN_08400 0.74 -0.2 417,488 + yadE HEPCGN_08400 0.74 -1.5 417,489 - yadE HEPCGN_08400 0.74 -0.0 417,590 - yadE HEPCGN_08400 0.83 -0.8 417,727 + -0.4 417,727 + -0.1 417,727 + +0.1 417,728 - +0.3 417,730 - +0.1 417,785 - -0.3 417,798 + +1.1 417,834 + -0.1 417,839 + +0.1 417,840 - +0.0 417,876 + -1.1 417,877 - -0.6 417,883 + -0.4 417,931 + yadI HEPCGN_08405 0.15 -0.8 417,962 - yadI HEPCGN_08405 0.22 +0.1 417,962 - yadI HEPCGN_08405 0.22 +0.7 418,073 + yadI HEPCGN_08405 0.47 -0.4 418,073 + yadI HEPCGN_08405 0.47 +0.1 418,106 + yadI HEPCGN_08405 0.54 +0.0 418,181 - yadI HEPCGN_08405 0.71 +0.8 418,183 + yadI HEPCGN_08405 0.72 -0.7 418,184 - yadI HEPCGN_08405 0.72 +0.5 418,184 - yadI HEPCGN_08405 0.72 +0.0 418,190 + yadI HEPCGN_08405 0.73 +2.2 418,260 - yadI HEPCGN_08405 0.89 +0.2 418,288 + -0.5 418,289 - +1.1 418,289 - +0.0 418,301 + -0.3 418,301 + -0.2 418,302 - -0.9 418,320 - -0.4 418,335 + -0.2 418,335 + +0.5 418,353 + +0.2 418,363 + -1.8 418,407 - -1.0 418,412 + -0.9 418,413 - +0.5 418,413 - -0.2 418,416 + -0.8 418,460 + -1.7 418,461 - +0.5 418,474 - -0.6 418,474 - +0.3 418,486 + -0.3 418,498 - HEPCGN_08410 0.11 -0.5 418,498 - HEPCGN_08410 0.11 -1.4 418,554 + HEPCGN_08410 0.19 -0.4 418,555 - HEPCGN_08410 0.19 +0.2 418,557 - HEPCGN_08410 0.19 -0.4 418,635 - HEPCGN_08410 0.29 -0.4 418,762 + HEPCGN_08410 0.46 -1.1
Or see this region's nucleotide sequence