Experiment: Bas19
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dmpG and mhpF overlap by 4 nucleotides mhpF and mhpD overlap by 4 nucleotides mhpD and mhpC are separated by 9 nucleotides mhpC and mhpB are separated by 17 nucleotides
HEPCGN_07385: dmpG - 4-hydroxy-2-oxovalerate aldolase, at 210,998 to 212,011
dmpG
HEPCGN_07390: mhpF - acetaldehyde dehydrogenase, at 212,008 to 212,958
mhpF
HEPCGN_07395: mhpD - 2-keto-4-pentenoate hydratase, at 212,955 to 213,764
mhpD
HEPCGN_07400: mhpC - 2-hydroxy-6-oxonona-2,4-dienedioate hydrolase, at 213,774 to 214,640
mhpC
HEPCGN_07405: mhpB - 3-carboxyethylcatechol 2,3-dioxygenase, at 214,658 to 215,602
mhpB
Position (kb) 212
213
214 Strain fitness (log2 ratio) -2
-1
0
1
2 at 211.963 kb on + strand at 211.963 kb on + strand at 211.963 kb on + strand at 211.964 kb on - strand at 212.083 kb on - strand at 212.187 kb on + strand, within mhpF at 212.197 kb on - strand, within mhpF at 212.252 kb on + strand, within mhpF at 212.252 kb on + strand, within mhpF at 212.253 kb on - strand, within mhpF at 212.324 kb on - strand, within mhpF at 212.324 kb on - strand, within mhpF at 212.327 kb on + strand, within mhpF at 212.327 kb on + strand, within mhpF at 212.328 kb on - strand, within mhpF at 212.368 kb on - strand, within mhpF at 212.487 kb on + strand, within mhpF at 212.507 kb on + strand, within mhpF at 212.508 kb on - strand, within mhpF at 212.631 kb on - strand, within mhpF at 212.740 kb on + strand, within mhpF at 212.741 kb on - strand, within mhpF at 212.741 kb on - strand, within mhpF at 212.741 kb on - strand, within mhpF at 212.741 kb on - strand, within mhpF at 212.811 kb on + strand, within mhpF at 213.020 kb on + strand at 213.066 kb on + strand, within mhpD at 213.067 kb on - strand, within mhpD at 213.067 kb on - strand, within mhpD at 213.078 kb on - strand, within mhpD at 213.087 kb on - strand, within mhpD at 213.275 kb on - strand, within mhpD at 213.275 kb on - strand, within mhpD at 213.275 kb on - strand, within mhpD at 213.275 kb on - strand, within mhpD at 213.275 kb on - strand, within mhpD at 213.275 kb on - strand, within mhpD at 213.293 kb on + strand, within mhpD at 213.385 kb on + strand, within mhpD at 213.385 kb on + strand, within mhpD at 213.386 kb on - strand, within mhpD at 213.418 kb on - strand, within mhpD at 213.418 kb on - strand, within mhpD at 213.519 kb on + strand, within mhpD at 213.520 kb on - strand, within mhpD at 214.055 kb on + strand, within mhpC at 214.056 kb on - strand, within mhpC at 214.056 kb on - strand, within mhpC at 214.056 kb on - strand, within mhpC at 214.056 kb on - strand, within mhpC at 214.056 kb on - strand, within mhpC at 214.056 kb on - strand, within mhpC at 214.178 kb on + strand, within mhpC at 214.179 kb on - strand, within mhpC at 214.286 kb on + strand, within mhpC at 214.286 kb on + strand, within mhpC at 214.286 kb on + strand, within mhpC at 214.286 kb on + strand, within mhpC at 214.286 kb on + strand, within mhpC at 214.286 kb on + strand, within mhpC at 214.287 kb on - strand, within mhpC at 214.556 kb on + strand at 214.557 kb on - strand at 214.557 kb on - strand at 214.655 kb on + strand at 214.719 kb on + strand at 214.720 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas19 remove 211,963 + +0.1 211,963 + -0.7 211,963 + +0.2 211,964 - -0.1 212,083 - +0.1 212,187 + mhpF HEPCGN_07390 0.19 -0.1 212,197 - mhpF HEPCGN_07390 0.20 -0.7 212,252 + mhpF HEPCGN_07390 0.26 +0.1 212,252 + mhpF HEPCGN_07390 0.26 -0.5 212,253 - mhpF HEPCGN_07390 0.26 -0.6 212,324 - mhpF HEPCGN_07390 0.33 -0.3 212,324 - mhpF HEPCGN_07390 0.33 +0.6 212,327 + mhpF HEPCGN_07390 0.34 +0.1 212,327 + mhpF HEPCGN_07390 0.34 -0.2 212,328 - mhpF HEPCGN_07390 0.34 -0.3 212,368 - mhpF HEPCGN_07390 0.38 -0.1 212,487 + mhpF HEPCGN_07390 0.50 +1.0 212,507 + mhpF HEPCGN_07390 0.52 +0.5 212,508 - mhpF HEPCGN_07390 0.53 +0.4 212,631 - mhpF HEPCGN_07390 0.66 -0.2 212,740 + mhpF HEPCGN_07390 0.77 -0.8 212,741 - mhpF HEPCGN_07390 0.77 -0.3 212,741 - mhpF HEPCGN_07390 0.77 -0.3 212,741 - mhpF HEPCGN_07390 0.77 +1.4 212,741 - mhpF HEPCGN_07390 0.77 +0.3 212,811 + mhpF HEPCGN_07390 0.84 -0.0 213,020 + -0.0 213,066 + mhpD HEPCGN_07395 0.14 -2.5 213,067 - mhpD HEPCGN_07395 0.14 +0.1 213,067 - mhpD HEPCGN_07395 0.14 -0.6 213,078 - mhpD HEPCGN_07395 0.15 +0.1 213,087 - mhpD HEPCGN_07395 0.16 -0.4 213,275 - mhpD HEPCGN_07395 0.40 +1.5 213,275 - mhpD HEPCGN_07395 0.40 +0.2 213,275 - mhpD HEPCGN_07395 0.40 -0.0 213,275 - mhpD HEPCGN_07395 0.40 -1.5 213,275 - mhpD HEPCGN_07395 0.40 +0.5 213,275 - mhpD HEPCGN_07395 0.40 -1.4 213,293 + mhpD HEPCGN_07395 0.42 +0.1 213,385 + mhpD HEPCGN_07395 0.53 -0.1 213,385 + mhpD HEPCGN_07395 0.53 -0.1 213,386 - mhpD HEPCGN_07395 0.53 -1.5 213,418 - mhpD HEPCGN_07395 0.57 -0.9 213,418 - mhpD HEPCGN_07395 0.57 -1.2 213,519 + mhpD HEPCGN_07395 0.70 -0.0 213,520 - mhpD HEPCGN_07395 0.70 +0.6 214,055 + mhpC HEPCGN_07400 0.32 -0.3 214,056 - mhpC HEPCGN_07400 0.33 +0.7 214,056 - mhpC HEPCGN_07400 0.33 -0.1 214,056 - mhpC HEPCGN_07400 0.33 -0.0 214,056 - mhpC HEPCGN_07400 0.33 -1.5 214,056 - mhpC HEPCGN_07400 0.33 -0.4 214,056 - mhpC HEPCGN_07400 0.33 -0.5 214,178 + mhpC HEPCGN_07400 0.47 -0.0 214,179 - mhpC HEPCGN_07400 0.47 -0.1 214,286 + mhpC HEPCGN_07400 0.59 -0.8 214,286 + mhpC HEPCGN_07400 0.59 -0.4 214,286 + mhpC HEPCGN_07400 0.59 +0.9 214,286 + mhpC HEPCGN_07400 0.59 +0.7 214,286 + mhpC HEPCGN_07400 0.59 -0.2 214,286 + mhpC HEPCGN_07400 0.59 -0.2 214,287 - mhpC HEPCGN_07400 0.59 -1.5 214,556 + -1.8 214,557 - -1.1 214,557 - +0.7 214,655 + +2.1 214,719 + +0.5 214,720 - -0.8
Or see this region's nucleotide sequence