Experiment: Bas19
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yoaC and yoaB are separated by 421 nucleotides yoaB and yoaA are separated by 128 nucleotides yoaA and tsaB are separated by 57 nucleotides tsaB and yeaY are separated by 38 nucleotides
HEPCGN_04960: yoaC - Uncharacterized protein YoaC, at 868,371 to 868,670
yoaC
HEPCGN_04965: yoaB - RutC family protein YoaB, at 869,092 to 869,439
yoaB
HEPCGN_04970: yoaA - putative ATP-dependent DNA helicase YoaA, at 869,568 to 871,478
yoaA
HEPCGN_04975: tsaB - tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB, at 871,536 to 872,231
tsaB
HEPCGN_04980: yeaY - Slp family lipoprotein YeaY, at 872,270 to 872,851
yeaY
Position (kb) 869
870
871
872 Strain fitness (log2 ratio) -2
-1
0
1
2 at 868.620 kb on + strand, within yoaC at 868.621 kb on - strand, within yoaC at 868.652 kb on + strand at 868.652 kb on + strand at 868.653 kb on - strand at 868.656 kb on + strand at 868.656 kb on + strand at 868.660 kb on - strand at 868.670 kb on + strand at 868.733 kb on - strand at 868.733 kb on - strand at 868.747 kb on + strand at 868.747 kb on + strand at 868.798 kb on - strand at 868.856 kb on + strand at 868.859 kb on - strand at 868.926 kb on + strand at 868.926 kb on + strand at 868.933 kb on - strand at 868.933 kb on - strand at 868.936 kb on + strand at 868.937 kb on - strand at 868.938 kb on + strand at 868.938 kb on + strand at 868.941 kb on - strand at 868.988 kb on + strand at 869.006 kb on + strand at 869.073 kb on + strand at 869.073 kb on + strand at 869.074 kb on - strand at 869.076 kb on + strand at 869.093 kb on + strand at 869.113 kb on - strand at 869.229 kb on + strand, within yoaB at 869.367 kb on - strand, within yoaB at 869.421 kb on + strand at 869.431 kb on - strand at 869.539 kb on - strand at 869.622 kb on - strand at 869.844 kb on + strand, within yoaA at 869.845 kb on - strand, within yoaA at 869.883 kb on + strand, within yoaA at 869.947 kb on + strand, within yoaA at 870.066 kb on - strand, within yoaA at 870.094 kb on - strand, within yoaA at 870.370 kb on + strand, within yoaA at 870.371 kb on - strand, within yoaA at 870.523 kb on - strand, within yoaA at 870.643 kb on - strand, within yoaA at 870.718 kb on + strand, within yoaA at 870.718 kb on + strand, within yoaA at 870.750 kb on - strand, within yoaA at 870.791 kb on + strand, within yoaA at 870.953 kb on + strand, within yoaA at 870.971 kb on + strand, within yoaA at 871.158 kb on - strand, within yoaA at 871.194 kb on - strand, within yoaA at 871.217 kb on - strand, within yoaA at 871.271 kb on - strand, within yoaA at 871.271 kb on - strand, within yoaA at 871.292 kb on + strand at 871.292 kb on + strand at 871.292 kb on + strand at 871.293 kb on - strand at 871.293 kb on - strand at 871.476 kb on + strand at 871.476 kb on + strand at 871.491 kb on + strand at 871.496 kb on + strand at 872.185 kb on - strand at 872.188 kb on + strand at 872.189 kb on - strand at 872.193 kb on - strand at 872.237 kb on - strand at 872.237 kb on - strand at 872.242 kb on + strand at 872.301 kb on + strand at 872.391 kb on + strand, within yeaY at 872.420 kb on + strand, within yeaY
Per-strain Table
Position Strand Gene LocusTag Fraction Bas19 remove 868,620 + yoaC HEPCGN_04960 0.83 +0.3 868,621 - yoaC HEPCGN_04960 0.83 +0.5 868,652 + +0.3 868,652 + -0.1 868,653 - +2.2 868,656 + +0.2 868,656 + +0.2 868,660 - +0.7 868,670 + +0.2 868,733 - -0.9 868,733 - -0.2 868,747 + -0.2 868,747 + -0.4 868,798 - +0.1 868,856 + -0.8 868,859 - -0.7 868,926 + +1.2 868,926 + -0.7 868,933 - -0.2 868,933 - +1.3 868,936 + -0.4 868,937 - -0.2 868,938 + +1.3 868,938 + -0.0 868,941 - -0.7 868,988 + -0.2 869,006 + +0.5 869,073 + -0.9 869,073 + -1.7 869,074 - -0.2 869,076 + -0.1 869,093 + +0.1 869,113 - +1.0 869,229 + yoaB HEPCGN_04965 0.39 -0.1 869,367 - yoaB HEPCGN_04965 0.79 -0.3 869,421 + +1.7 869,431 - -0.3 869,539 - +0.2 869,622 - +0.1 869,844 + yoaA HEPCGN_04970 0.14 -0.2 869,845 - yoaA HEPCGN_04970 0.14 +0.7 869,883 + yoaA HEPCGN_04970 0.16 +0.6 869,947 + yoaA HEPCGN_04970 0.20 +0.3 870,066 - yoaA HEPCGN_04970 0.26 -0.4 870,094 - yoaA HEPCGN_04970 0.28 -0.3 870,370 + yoaA HEPCGN_04970 0.42 -0.0 870,371 - yoaA HEPCGN_04970 0.42 -0.7 870,523 - yoaA HEPCGN_04970 0.50 -0.7 870,643 - yoaA HEPCGN_04970 0.56 -0.2 870,718 + yoaA HEPCGN_04970 0.60 -0.7 870,718 + yoaA HEPCGN_04970 0.60 -1.4 870,750 - yoaA HEPCGN_04970 0.62 -0.3 870,791 + yoaA HEPCGN_04970 0.64 -0.3 870,953 + yoaA HEPCGN_04970 0.72 +0.1 870,971 + yoaA HEPCGN_04970 0.73 -1.8 871,158 - yoaA HEPCGN_04970 0.83 +0.2 871,194 - yoaA HEPCGN_04970 0.85 +0.9 871,217 - yoaA HEPCGN_04970 0.86 -0.5 871,271 - yoaA HEPCGN_04970 0.89 -0.5 871,271 - yoaA HEPCGN_04970 0.89 -0.9 871,292 + +0.3 871,292 + -0.7 871,292 + -0.8 871,293 - +0.2 871,293 - -0.3 871,476 + -0.6 871,476 + -0.1 871,491 + -1.1 871,496 + +0.7 872,185 - +1.7 872,188 + -0.9 872,189 - +0.1 872,193 - +0.5 872,237 - -0.7 872,237 - +1.7 872,242 + +0.6 872,301 + +0.2 872,391 + yeaY HEPCGN_04980 0.21 +1.1 872,420 + yeaY HEPCGN_04980 0.26 -0.6
Or see this region's nucleotide sequence