Experiment: Bas19
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt atpA and atpG are separated by 50 nucleotides atpG and atpD are separated by 26 nucleotides atpD and atpC are separated by 20 nucleotides atpC and glmU are separated by 348 nucleotides
HEPCGN_13535: atpA - F0F1 ATP synthase subunit alpha, at 1,452,228 to 1,453,769
atpA
HEPCGN_13540: atpG - F0F1 ATP synthase subunit gamma, at 1,453,820 to 1,454,683
atpG
HEPCGN_13545: atpD - F0F1 ATP synthase subunit beta, at 1,454,710 to 1,456,092
atpD
HEPCGN_13550: atpC - F0F1 ATP synthase subunit epsilon, at 1,456,113 to 1,456,532
atpC
HEPCGN_13560: glmU - bifunctional UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase GlmU, at 1,456,881 to 1,458,251
glmU
Position (kb)
1454
1455
1456
1457 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1453.843 kb on + strand at 1454.344 kb on + strand, within atpG at 1454.422 kb on + strand, within atpG at 1454.612 kb on + strand at 1454.690 kb on + strand at 1455.601 kb on + strand, within atpD at 1456.015 kb on + strand at 1456.042 kb on + strand at 1456.042 kb on + strand at 1456.412 kb on + strand, within atpC at 1456.451 kb on - strand, within atpC at 1456.455 kb on + strand, within atpC at 1456.455 kb on + strand, within atpC at 1456.530 kb on + strand at 1456.614 kb on + strand at 1456.615 kb on - strand at 1456.630 kb on - strand at 1456.630 kb on - strand at 1456.630 kb on - strand at 1456.630 kb on - strand at 1456.668 kb on - strand at 1456.704 kb on + strand at 1456.705 kb on - strand at 1456.772 kb on + strand at 1456.865 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas19 remove 1,453,843 + +1.4 1,454,344 + atpG HEPCGN_13540 0.61 +1.4 1,454,422 + atpG HEPCGN_13540 0.70 +0.8 1,454,612 + +2.4 1,454,690 + -1.7 1,455,601 + atpD HEPCGN_13545 0.64 +1.4 1,456,015 + +0.4 1,456,042 + +1.4 1,456,042 + +0.1 1,456,412 + atpC HEPCGN_13550 0.71 -1.2 1,456,451 - atpC HEPCGN_13550 0.80 +1.4 1,456,455 + atpC HEPCGN_13550 0.81 -0.8 1,456,455 + atpC HEPCGN_13550 0.81 -2.1 1,456,530 + +0.7 1,456,614 + -1.6 1,456,615 - -0.2 1,456,630 - +0.4 1,456,630 - +1.0 1,456,630 - +0.4 1,456,630 - +0.2 1,456,668 - -1.6 1,456,704 + -0.6 1,456,705 - -1.6 1,456,772 + -0.6 1,456,865 + -3.3
Or see this region's nucleotide sequence