Experiment: Bas19
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt argH and HEPCGN_12225 are separated by 60 nucleotides HEPCGN_12225 and argC are separated by 7 nucleotides
HEPCGN_12220: argH - argininosuccinate lyase, at 1,184,587 to 1,185,960
argH
HEPCGN_12225: HEPCGN_12225 - acetylglutamate kinase, at 1,186,021 to 1,186,797
_12225
HEPCGN_12230: argC - N-acetyl-gamma-glutamyl-phosphate reductase, at 1,186,805 to 1,187,809
argC
Position (kb)
1186
1187 Strain fitness (log2 ratio)
-2
-1
0
1 at 1185.325 kb on + strand, within argH at 1185.325 kb on + strand, within argH at 1185.326 kb on - strand, within argH at 1185.447 kb on - strand, within argH at 1185.501 kb on - strand, within argH at 1185.705 kb on + strand, within argH at 1185.705 kb on + strand, within argH at 1185.705 kb on + strand, within argH at 1185.705 kb on + strand, within argH at 1185.706 kb on - strand, within argH at 1185.706 kb on - strand, within argH at 1185.706 kb on - strand, within argH at 1185.712 kb on - strand, within argH at 1185.862 kb on - strand at 1185.960 kb on + strand at 1185.960 kb on + strand at 1185.961 kb on - strand at 1185.975 kb on - strand at 1186.022 kb on + strand at 1186.050 kb on + strand at 1186.050 kb on + strand at 1186.051 kb on - strand at 1186.222 kb on - strand, within HEPCGN_12225 at 1186.222 kb on - strand, within HEPCGN_12225 at 1186.401 kb on + strand, within HEPCGN_12225 at 1186.402 kb on - strand, within HEPCGN_12225 at 1186.441 kb on + strand, within HEPCGN_12225 at 1186.499 kb on - strand, within HEPCGN_12225 at 1186.499 kb on - strand, within HEPCGN_12225 at 1186.579 kb on + strand, within HEPCGN_12225 at 1186.658 kb on - strand, within HEPCGN_12225 at 1186.725 kb on + strand at 1186.779 kb on + strand at 1186.780 kb on - strand at 1186.784 kb on - strand at 1186.807 kb on - strand at 1186.810 kb on + strand at 1186.811 kb on - strand at 1186.831 kb on - strand at 1186.856 kb on + strand at 1186.857 kb on - strand at 1186.906 kb on + strand, within argC at 1186.906 kb on + strand, within argC at 1186.992 kb on + strand, within argC at 1186.992 kb on + strand, within argC at 1187.012 kb on - strand, within argC at 1187.019 kb on + strand, within argC at 1187.019 kb on + strand, within argC at 1187.020 kb on - strand, within argC at 1187.030 kb on + strand, within argC at 1187.030 kb on + strand, within argC at 1187.218 kb on + strand, within argC at 1187.236 kb on + strand, within argC at 1187.263 kb on + strand, within argC at 1187.413 kb on - strand, within argC at 1187.461 kb on - strand, within argC at 1187.461 kb on - strand, within argC at 1187.470 kb on - strand, within argC at 1187.470 kb on - strand, within argC at 1187.546 kb on + strand, within argC at 1187.655 kb on - strand, within argC at 1187.655 kb on - strand, within argC at 1187.731 kb on + strand at 1187.747 kb on + strand at 1187.751 kb on + strand at 1187.752 kb on - strand at 1187.752 kb on - strand at 1187.759 kb on + strand at 1187.759 kb on + strand at 1187.760 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas19 remove 1,185,325 + argH HEPCGN_12220 0.54 -0.4 1,185,325 + argH HEPCGN_12220 0.54 -0.2 1,185,326 - argH HEPCGN_12220 0.54 -0.6 1,185,447 - argH HEPCGN_12220 0.63 -0.4 1,185,501 - argH HEPCGN_12220 0.67 +0.5 1,185,705 + argH HEPCGN_12220 0.81 -0.6 1,185,705 + argH HEPCGN_12220 0.81 +0.1 1,185,705 + argH HEPCGN_12220 0.81 +0.0 1,185,705 + argH HEPCGN_12220 0.81 +0.0 1,185,706 - argH HEPCGN_12220 0.81 -0.6 1,185,706 - argH HEPCGN_12220 0.81 +0.8 1,185,706 - argH HEPCGN_12220 0.81 +0.9 1,185,712 - argH HEPCGN_12220 0.82 -0.1 1,185,862 - -0.7 1,185,960 + -2.4 1,185,960 + +0.0 1,185,961 - +0.1 1,185,975 - -2.1 1,186,022 + -0.4 1,186,050 + +0.6 1,186,050 + -0.2 1,186,051 - +0.0 1,186,222 - HEPCGN_12225 0.26 -0.2 1,186,222 - HEPCGN_12225 0.26 -0.2 1,186,401 + HEPCGN_12225 0.49 +0.1 1,186,402 - HEPCGN_12225 0.49 -0.5 1,186,441 + HEPCGN_12225 0.54 -1.1 1,186,499 - HEPCGN_12225 0.62 -0.3 1,186,499 - HEPCGN_12225 0.62 +0.2 1,186,579 + HEPCGN_12225 0.72 +1.3 1,186,658 - HEPCGN_12225 0.82 -0.5 1,186,725 + -1.1 1,186,779 + -0.2 1,186,780 - +1.5 1,186,784 - +0.9 1,186,807 - -0.5 1,186,810 + +0.4 1,186,811 - -1.5 1,186,831 - -0.0 1,186,856 + +0.2 1,186,857 - -0.6 1,186,906 + argC HEPCGN_12230 0.10 -0.3 1,186,906 + argC HEPCGN_12230 0.10 -0.8 1,186,992 + argC HEPCGN_12230 0.19 +0.1 1,186,992 + argC HEPCGN_12230 0.19 +0.4 1,187,012 - argC HEPCGN_12230 0.21 -1.0 1,187,019 + argC HEPCGN_12230 0.21 -0.3 1,187,019 + argC HEPCGN_12230 0.21 +0.4 1,187,020 - argC HEPCGN_12230 0.21 -0.5 1,187,030 + argC HEPCGN_12230 0.22 -0.5 1,187,030 + argC HEPCGN_12230 0.22 +0.2 1,187,218 + argC HEPCGN_12230 0.41 -1.1 1,187,236 + argC HEPCGN_12230 0.43 -0.5 1,187,263 + argC HEPCGN_12230 0.46 -0.5 1,187,413 - argC HEPCGN_12230 0.60 -0.9 1,187,461 - argC HEPCGN_12230 0.65 -0.6 1,187,461 - argC HEPCGN_12230 0.65 +0.3 1,187,470 - argC HEPCGN_12230 0.66 -0.9 1,187,470 - argC HEPCGN_12230 0.66 -0.5 1,187,546 + argC HEPCGN_12230 0.74 +0.2 1,187,655 - argC HEPCGN_12230 0.85 -0.5 1,187,655 - argC HEPCGN_12230 0.85 -0.4 1,187,731 + +0.2 1,187,747 + -1.2 1,187,751 + +0.4 1,187,752 - -0.1 1,187,752 - +1.2 1,187,759 + +0.2 1,187,759 + +0.9 1,187,760 - +0.5
Or see this region's nucleotide sequence