Experiment: Bas19
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt blc and sugE overlap by 4 nucleotides sugE and HEPCGN_10605 are separated by 174 nucleotides HEPCGN_10605 and ecnA are separated by 110 nucleotides ecnA and HEPCGN_10615 are separated by 51 nucleotides HEPCGN_10615 and epmB are separated by 41 nucleotides
HEPCGN_10595: blc - lipocalin Blc, at 861,783 to 862,316
blc
HEPCGN_10600: sugE - quaternary ammonium compound efflux SMR transporter SugE, at 862,313 to 862,630
sugE
HEPCGN_10605: HEPCGN_10605 - Entericidin B, at 862,805 to 862,951
_10605
HEPCGN_10610: ecnA - lipoprotein antitoxin entericidin A, at 863,062 to 863,187
ecnA
HEPCGN_10615: HEPCGN_10615 - Elongation factor P, at 863,239 to 863,805
_10615
HEPCGN_10620: epmB - EF-P beta-lysylation protein EpmB, at 863,847 to 864,875
epmB
Position (kb)
863
864 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 862.062 kb on + strand, within blc at 862.129 kb on - strand, within blc at 862.131 kb on - strand, within blc at 862.131 kb on - strand, within blc at 862.136 kb on + strand, within blc at 862.191 kb on + strand, within blc at 862.192 kb on - strand, within blc at 862.202 kb on - strand, within blc at 862.283 kb on + strand at 862.284 kb on - strand at 862.284 kb on - strand at 862.289 kb on + strand at 862.297 kb on + strand at 862.339 kb on + strand at 862.340 kb on - strand at 862.529 kb on - strand, within sugE at 862.566 kb on + strand, within sugE at 862.610 kb on - strand at 862.618 kb on + strand at 862.618 kb on + strand at 862.618 kb on + strand at 862.631 kb on - strand at 862.742 kb on + strand at 862.785 kb on - strand at 862.793 kb on + strand at 862.799 kb on + strand at 862.806 kb on + strand at 862.895 kb on + strand, within HEPCGN_10605 at 862.895 kb on + strand, within HEPCGN_10605 at 862.896 kb on - strand, within HEPCGN_10605 at 862.896 kb on - strand, within HEPCGN_10605 at 862.970 kb on - strand at 862.980 kb on - strand at 862.991 kb on + strand at 863.002 kb on + strand at 863.005 kb on + strand at 863.006 kb on - strand at 863.101 kb on - strand, within ecnA at 863.200 kb on + strand at 863.201 kb on - strand at 863.240 kb on + strand at 863.241 kb on - strand at 863.258 kb on + strand at 863.259 kb on - strand at 863.580 kb on - strand, within HEPCGN_10615 at 863.586 kb on - strand, within HEPCGN_10615 at 863.586 kb on - strand, within HEPCGN_10615 at 863.792 kb on + strand at 863.793 kb on - strand at 863.796 kb on - strand at 863.832 kb on + strand at 863.836 kb on + strand at 863.836 kb on + strand at 863.837 kb on - strand at 863.855 kb on + strand at 863.856 kb on - strand at 863.866 kb on + strand at 863.866 kb on + strand at 863.866 kb on + strand at 863.867 kb on - strand at 863.870 kb on + strand at 863.949 kb on - strand at 863.950 kb on + strand, within epmB at 864.023 kb on + strand, within epmB at 864.077 kb on + strand, within epmB at 864.077 kb on + strand, within epmB at 864.145 kb on + strand, within epmB at 864.145 kb on + strand, within epmB at 864.164 kb on + strand, within epmB at 864.164 kb on + strand, within epmB at 864.165 kb on - strand, within epmB
Per-strain Table
Position Strand Gene LocusTag Fraction Bas19 remove 862,062 + blc HEPCGN_10595 0.52 -0.8 862,129 - blc HEPCGN_10595 0.65 +0.1 862,131 - blc HEPCGN_10595 0.65 +0.5 862,131 - blc HEPCGN_10595 0.65 +0.7 862,136 + blc HEPCGN_10595 0.66 +0.5 862,191 + blc HEPCGN_10595 0.76 -1.4 862,192 - blc HEPCGN_10595 0.77 -0.5 862,202 - blc HEPCGN_10595 0.78 +0.4 862,283 + -0.1 862,284 - -0.1 862,284 - -0.8 862,289 + -0.7 862,297 + +0.2 862,339 + +0.4 862,340 - -0.6 862,529 - sugE HEPCGN_10600 0.68 +0.2 862,566 + sugE HEPCGN_10600 0.80 -1.2 862,610 - -0.8 862,618 + +0.2 862,618 + -0.6 862,618 + -0.2 862,631 - -0.3 862,742 + -0.4 862,785 - +0.2 862,793 + -1.0 862,799 + +2.0 862,806 + +1.2 862,895 + HEPCGN_10605 0.61 +0.5 862,895 + HEPCGN_10605 0.61 +0.3 862,896 - HEPCGN_10605 0.62 -0.8 862,896 - HEPCGN_10605 0.62 -0.1 862,970 - +0.2 862,980 - -0.0 862,991 + -1.0 863,002 + +0.5 863,005 + -0.2 863,006 - -0.1 863,101 - ecnA HEPCGN_10610 0.31 +1.0 863,200 + -0.6 863,201 - +0.1 863,240 + -0.6 863,241 - -0.9 863,258 + -0.8 863,259 - +2.2 863,580 - HEPCGN_10615 0.60 +0.6 863,586 - HEPCGN_10615 0.61 -0.6 863,586 - HEPCGN_10615 0.61 +2.2 863,792 + +0.6 863,793 - -0.4 863,796 - -0.1 863,832 + +0.6 863,836 + +0.6 863,836 + -0.4 863,837 - -0.4 863,855 + -0.6 863,856 - -0.1 863,866 + -3.1 863,866 + -0.9 863,866 + -4.5 863,867 - -3.4 863,870 + -0.1 863,949 - +0.6 863,950 + epmB HEPCGN_10620 0.10 -2.4 864,023 + epmB HEPCGN_10620 0.17 -2.0 864,077 + epmB HEPCGN_10620 0.22 -2.0 864,077 + epmB HEPCGN_10620 0.22 -4.4 864,145 + epmB HEPCGN_10620 0.29 -3.1 864,145 + epmB HEPCGN_10620 0.29 -1.7 864,164 + epmB HEPCGN_10620 0.31 +0.6 864,164 + epmB HEPCGN_10620 0.31 -1.9 864,165 - epmB HEPCGN_10620 0.31 -2.2
Or see this region's nucleotide sequence