Strain Fitness in Escherichia coli ECOR38 around HEPCGN_07045

Experiment: Bas19

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntthiI and xseB are separated by 205 nucleotidesxseB and ispA overlap by 1 nucleotidesispA and dxs are separated by 24 nucleotides HEPCGN_07035: thiI - tRNA sulfurtransferase, at 142,514 to 143,962 thiI HEPCGN_07040: xseB - exodeoxyribonuclease VII small subunit, at 144,168 to 144,410 xseB HEPCGN_07045: ispA - (2E,6E)-farnesyl diphosphate synthase, at 144,410 to 145,309 ispA HEPCGN_07050: dxs - 1-deoxy-D-xylulose-5-phosphate synthase, at 145,334 to 147,196 dxs Position (kb) 144 145 146Strain fitness (log2 ratio) -1 0 1at 143.437 kb on + strand, within thiIat 143.476 kb on + strand, within thiIat 143.696 kb on - strand, within thiIat 143.738 kb on - strand, within thiIat 143.853 kb on + strandat 143.854 kb on - strandat 143.947 kb on + strandat 143.954 kb on - strandat 144.000 kb on + strandat 144.000 kb on + strandat 144.001 kb on - strandat 144.052 kb on + strandat 144.052 kb on + strandat 144.052 kb on + strandat 144.053 kb on - strandat 144.053 kb on - strandat 144.054 kb on + strandat 144.054 kb on + strandat 144.089 kb on - strandat 144.089 kb on - strandat 144.101 kb on + strandat 144.108 kb on + strandat 144.108 kb on + strandat 144.167 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas19
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143,437 + thiI HEPCGN_07035 0.64 -0.7
143,476 + thiI HEPCGN_07035 0.66 +1.0
143,696 - thiI HEPCGN_07035 0.82 -1.3
143,738 - thiI HEPCGN_07035 0.84 -1.2
143,853 + +0.6
143,854 - -0.2
143,947 + -1.6
143,954 - -1.4
144,000 + -0.2
144,000 + +0.2
144,001 - -0.5
144,052 + -1.0
144,052 + -1.4
144,052 + -0.1
144,053 - -0.2
144,053 - -0.1
144,054 + -0.0
144,054 + -1.7
144,089 - -0.7
144,089 - -0.0
144,101 + +0.2
144,108 + +1.1
144,108 + -0.5
144,167 + -0.5

Or see this region's nucleotide sequence