Experiment: Bas19
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thiI and xseB are separated by 205 nucleotides xseB and ispA overlap by 1 nucleotides ispA and dxs are separated by 24 nucleotides
HEPCGN_07035: thiI - tRNA sulfurtransferase, at 142,514 to 143,962
thiI
HEPCGN_07040: xseB - exodeoxyribonuclease VII small subunit, at 144,168 to 144,410
xseB
HEPCGN_07045: ispA - (2E,6E)-farnesyl diphosphate synthase, at 144,410 to 145,309
ispA
HEPCGN_07050: dxs - 1-deoxy-D-xylulose-5-phosphate synthase, at 145,334 to 147,196
dxs
Position (kb)
144
145
146 Strain fitness (log2 ratio)
-1
0
1 at 143.437 kb on + strand, within thiI at 143.476 kb on + strand, within thiI at 143.696 kb on - strand, within thiI at 143.738 kb on - strand, within thiI at 143.853 kb on + strand at 143.854 kb on - strand at 143.947 kb on + strand at 143.954 kb on - strand at 144.000 kb on + strand at 144.000 kb on + strand at 144.001 kb on - strand at 144.052 kb on + strand at 144.052 kb on + strand at 144.052 kb on + strand at 144.053 kb on - strand at 144.053 kb on - strand at 144.054 kb on + strand at 144.054 kb on + strand at 144.089 kb on - strand at 144.089 kb on - strand at 144.101 kb on + strand at 144.108 kb on + strand at 144.108 kb on + strand at 144.167 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas19 remove 143,437 + thiI HEPCGN_07035 0.64 -0.7 143,476 + thiI HEPCGN_07035 0.66 +1.0 143,696 - thiI HEPCGN_07035 0.82 -1.3 143,738 - thiI HEPCGN_07035 0.84 -1.2 143,853 + +0.6 143,854 - -0.2 143,947 + -1.6 143,954 - -1.4 144,000 + -0.2 144,000 + +0.2 144,001 - -0.5 144,052 + -1.0 144,052 + -1.4 144,052 + -0.1 144,053 - -0.2 144,053 - -0.1 144,054 + -0.0 144,054 + -1.7 144,089 - -0.7 144,089 - -0.0 144,101 + +0.2 144,108 + +1.1 144,108 + -0.5 144,167 + -0.5
Or see this region's nucleotide sequence