Experiment: Bas19
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt uvrC and pgsA are separated by 56 nucleotides pgsA and Gly_trna are separated by 151 nucleotides Gly_trna and Cys_trna are separated by 54 nucleotides Cys_trna and Leu_trna are separated by 12 nucleotides
HEPCGN_04180: uvrC - excinuclease ABC subunit UvrC, at 733,486 to 735,318
uvrC
HEPCGN_04185: pgsA - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase, at 735,375 to 735,923
pgsA
HEPCGN_04190: Gly_trna - tRNA-Gly(gcc), at 736,075 to 736,150
_trna
HEPCGN_04195: Cys_trna - tRNA-Cys(gca), at 736,205 to 736,278
_trna
HEPCGN_04200: Leu_trna - tRNA-Leu(taa), at 736,291 to 736,377
_trna
Position (kb) 736
737 Strain fitness (log2 ratio) -1
0
1
2 at 735.364 kb on + strand at 736.508 kb on + strand at 736.596 kb on - strand at 736.724 kb on + strand at 736.777 kb on + strand at 736.791 kb on - strand at 736.820 kb on + strand at 736.820 kb on + strand at 736.821 kb on - strand at 736.821 kb on - strand at 736.857 kb on - strand at 736.857 kb on - strand at 736.896 kb on + strand at 736.899 kb on + strand at 737.013 kb on + strand at 737.018 kb on - strand at 737.060 kb on + strand at 737.062 kb on + strand at 737.083 kb on + strand at 737.084 kb on - strand at 737.084 kb on - strand at 737.084 kb on - strand at 737.084 kb on - strand at 737.105 kb on + strand at 737.106 kb on - strand at 737.106 kb on - strand at 737.106 kb on - strand at 737.107 kb on + strand at 737.133 kb on + strand at 737.133 kb on + strand at 737.133 kb on + strand at 737.134 kb on - strand at 737.229 kb on - strand at 737.244 kb on + strand at 737.249 kb on + strand at 737.250 kb on - strand at 737.250 kb on - strand at 737.259 kb on + strand at 737.344 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas19 remove 735,364 + +0.6 736,508 + -0.7 736,596 - +0.7 736,724 + -0.5 736,777 + -0.8 736,791 - +1.0 736,820 + -0.6 736,820 + -0.4 736,821 - -0.4 736,821 - -0.7 736,857 - -0.3 736,857 - -0.4 736,896 + +0.9 736,899 + +0.9 737,013 + -0.3 737,018 - +0.6 737,060 + +1.5 737,062 + -0.1 737,083 + +0.2 737,084 - +0.1 737,084 - +0.6 737,084 - -0.2 737,084 - -0.4 737,105 + +0.1 737,106 - +0.2 737,106 - -0.6 737,106 - +2.6 737,107 + +0.0 737,133 + -0.3 737,133 + +1.1 737,133 + -1.1 737,134 - -0.6 737,229 - +0.0 737,244 + -0.4 737,249 + +2.2 737,250 - -0.4 737,250 - +0.7 737,259 + +0.0 737,344 - -0.2
Or see this region's nucleotide sequence