Experiment: T4 MOI1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OHPLBJKB_00529 and OHPLBJKB_00530 are separated by 236 nucleotides OHPLBJKB_00530 and OHPLBJKB_00531 are separated by 27 nucleotides OHPLBJKB_00531 and OHPLBJKB_00532 are separated by 24 nucleotides
OHPLBJKB_00529: OHPLBJKB_00529 - tRNA-Met, at 541,686 to 541,762
_00529
OHPLBJKB_00530: OHPLBJKB_00530 - Ribosome maturation factor RimP, at 541,999 to 542,421
_00530
OHPLBJKB_00531: OHPLBJKB_00531 - Transcription termination/antitermination protein NusA, at 542,449 to 543,936
_00531
OHPLBJKB_00532: OHPLBJKB_00532 - Translation initiation factor IF-2, at 543,961 to 546,633
_00532
Position (kb) 542
543
544 Strain fitness (log2 ratio) -2
-1
0
1 at 541.456 kb on + strand at 541.456 kb on + strand at 541.475 kb on + strand at 541.511 kb on - strand at 541.553 kb on - strand at 541.589 kb on - strand at 541.589 kb on - strand at 541.639 kb on + strand at 541.639 kb on + strand at 541.640 kb on - strand at 541.640 kb on - strand at 541.771 kb on + strand at 541.788 kb on + strand at 541.790 kb on + strand at 541.790 kb on + strand at 541.881 kb on + strand at 543.604 kb on + strand, within OHPLBJKB_00531 at 543.604 kb on + strand, within OHPLBJKB_00531 at 543.604 kb on + strand, within OHPLBJKB_00531 at 543.604 kb on + strand, within OHPLBJKB_00531 at 543.767 kb on + strand, within OHPLBJKB_00531 at 543.767 kb on + strand, within OHPLBJKB_00531 at 543.810 kb on + strand at 543.810 kb on + strand at 543.909 kb on + strand at 543.909 kb on + strand at 543.934 kb on + strand at 543.934 kb on + strand at 543.937 kb on + strand at 543.944 kb on + strand at 543.944 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction T4 MOI1 remove 541,456 + +0.4 541,456 + +0.1 541,475 + -0.4 541,511 - +0.9 541,553 - -1.4 541,589 - -0.7 541,589 - +0.9 541,639 + +1.0 541,639 + -0.2 541,640 - +0.4 541,640 - +0.2 541,771 + -0.7 541,788 + -1.3 541,790 + +1.2 541,790 + -1.6 541,881 + -0.6 543,604 + OHPLBJKB_00531 0.78 -0.8 543,604 + OHPLBJKB_00531 0.78 -1.4 543,604 + OHPLBJKB_00531 0.78 -1.0 543,604 + OHPLBJKB_00531 0.78 -1.4 543,767 + OHPLBJKB_00531 0.89 +0.4 543,767 + OHPLBJKB_00531 0.89 +0.7 543,810 + +1.0 543,810 + -0.2 543,909 + -2.2 543,909 + -1.8 543,934 + -0.9 543,934 + -0.8 543,937 + +0.2 543,944 + +0.5 543,944 + -0.4
Or see this region's nucleotide sequence