Experiment: T4 MOI1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OHPLBJKB_00073 and OHPLBJKB_00074 are separated by 16 nucleotides OHPLBJKB_00074 and OHPLBJKB_00075 are separated by 9 nucleotides OHPLBJKB_00075 and OHPLBJKB_00076 are separated by 25 nucleotides
OHPLBJKB_00073: OHPLBJKB_00073 - hypothetical protein, at 76,178 to 77,194
_00073
OHPLBJKB_00074: OHPLBJKB_00074 - hypothetical protein, at 77,211 to 78,206
_00074
OHPLBJKB_00075: OHPLBJKB_00075 - Lipopolysaccharide core heptose(II) kinase RfaY, at 78,216 to 78,908
_00075
OHPLBJKB_00076: OHPLBJKB_00076 - General stress protein A, at 78,934 to 79,959
_00076
Position (kb) 77
78
79 Strain fitness (log2 ratio) -2
-1
0
1 at 76.339 kb on + strand, within OHPLBJKB_00073 at 76.663 kb on + strand, within OHPLBJKB_00073 at 77.278 kb on + strand at 77.458 kb on + strand, within OHPLBJKB_00074 at 77.523 kb on + strand, within OHPLBJKB_00074 at 77.748 kb on + strand, within OHPLBJKB_00074 at 77.794 kb on + strand, within OHPLBJKB_00074 at 77.838 kb on + strand, within OHPLBJKB_00074 at 77.838 kb on + strand, within OHPLBJKB_00074 at 77.882 kb on + strand, within OHPLBJKB_00074 at 78.008 kb on + strand, within OHPLBJKB_00074 at 78.008 kb on + strand, within OHPLBJKB_00074 at 78.221 kb on + strand at 78.230 kb on - strand at 78.680 kb on + strand, within OHPLBJKB_00075 at 78.680 kb on + strand, within OHPLBJKB_00075 at 78.681 kb on - strand, within OHPLBJKB_00075 at 78.681 kb on - strand, within OHPLBJKB_00075 at 78.907 kb on - strand at 78.907 kb on - strand at 78.941 kb on + strand at 79.009 kb on - strand at 79.015 kb on + strand at 79.056 kb on + strand, within OHPLBJKB_00076 at 79.056 kb on + strand, within OHPLBJKB_00076
Per-strain Table
Position Strand Gene LocusTag Fraction T4 MOI1 remove 76,339 + OHPLBJKB_00073 0.16 -0.5 76,663 + OHPLBJKB_00073 0.48 -0.8 77,278 + -1.1 77,458 + OHPLBJKB_00074 0.25 -1.4 77,523 + OHPLBJKB_00074 0.31 -1.9 77,748 + OHPLBJKB_00074 0.54 -1.0 77,794 + OHPLBJKB_00074 0.59 +0.2 77,838 + OHPLBJKB_00074 0.63 -1.1 77,838 + OHPLBJKB_00074 0.63 -1.8 77,882 + OHPLBJKB_00074 0.67 -0.8 78,008 + OHPLBJKB_00074 0.80 +0.3 78,008 + OHPLBJKB_00074 0.80 +0.5 78,221 + -0.0 78,230 - -0.3 78,680 + OHPLBJKB_00075 0.67 +0.5 78,680 + OHPLBJKB_00075 0.67 -0.5 78,681 - OHPLBJKB_00075 0.67 -0.5 78,681 - OHPLBJKB_00075 0.67 -1.1 78,907 - -0.4 78,907 - +0.2 78,941 + +0.0 79,009 - -0.3 79,015 + +0.6 79,056 + OHPLBJKB_00076 0.12 -0.4 79,056 + OHPLBJKB_00076 0.12 -1.3
Or see this region's nucleotide sequence