Experiment: R2A_PIPES with Metal_ion_mix_sitestressplate 2 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt secD and yajC are separated by 100 nucleotides yajC and tgt are separated by 165 nucleotides tgt and queA are separated by 129 nucleotides
OKGIIK_09990: secD - protein translocase subunit SecD, at 2,156,415 to 2,158,298
secD
OKGIIK_09995: yajC - preprotein translocase subunit YajC, at 2,158,399 to 2,158,728
yajC
OKGIIK_10000: tgt - tRNA guanosine(34) transglycosylase Tgt, at 2,158,894 to 2,160,036
tgt
OKGIIK_10005: queA - tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA, at 2,160,166 to 2,161,230
queA
Position (kb)
2158
2159
2160
2161 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2158.626 kb on - strand, within yajC at 2158.626 kb on - strand, within yajC at 2158.626 kb on - strand, within yajC at 2158.626 kb on - strand, within yajC at 2158.626 kb on - strand, within yajC at 2158.626 kb on - strand, within yajC at 2158.626 kb on - strand, within yajC at 2158.746 kb on - strand at 2158.746 kb on - strand at 2158.796 kb on - strand at 2158.796 kb on - strand at 2158.895 kb on + strand at 2158.896 kb on - strand at 2158.925 kb on + strand at 2158.925 kb on + strand at 2158.925 kb on + strand at 2158.925 kb on + strand at 2158.926 kb on - strand at 2158.926 kb on - strand at 2158.926 kb on - strand at 2158.926 kb on - strand at 2158.997 kb on + strand at 2158.998 kb on - strand at 2158.998 kb on - strand at 2159.000 kb on + strand at 2159.001 kb on - strand at 2159.001 kb on - strand at 2159.069 kb on + strand, within tgt at 2159.069 kb on + strand, within tgt at 2159.099 kb on + strand, within tgt at 2159.099 kb on + strand, within tgt at 2159.099 kb on + strand, within tgt at 2159.100 kb on - strand, within tgt at 2159.141 kb on + strand, within tgt at 2159.141 kb on + strand, within tgt at 2159.141 kb on + strand, within tgt at 2159.141 kb on + strand, within tgt at 2159.141 kb on + strand, within tgt at 2159.141 kb on + strand, within tgt at 2159.142 kb on - strand, within tgt at 2159.142 kb on - strand, within tgt at 2159.142 kb on - strand, within tgt at 2159.142 kb on - strand, within tgt at 2159.142 kb on - strand, within tgt at 2159.288 kb on + strand, within tgt at 2159.288 kb on + strand, within tgt at 2159.288 kb on + strand, within tgt at 2159.288 kb on + strand, within tgt at 2159.289 kb on - strand, within tgt at 2159.385 kb on - strand, within tgt at 2159.385 kb on - strand, within tgt at 2159.385 kb on - strand, within tgt at 2159.441 kb on + strand, within tgt at 2159.441 kb on + strand, within tgt at 2159.441 kb on + strand, within tgt at 2159.441 kb on + strand, within tgt at 2159.442 kb on - strand, within tgt at 2159.442 kb on - strand, within tgt at 2159.924 kb on + strand at 2159.924 kb on + strand at 2159.925 kb on - strand at 2159.925 kb on - strand at 2159.925 kb on - strand at 2159.925 kb on - strand at 2159.925 kb on - strand at 2160.079 kb on - strand at 2160.079 kb on - strand at 2160.079 kb on - strand at 2160.079 kb on - strand at 2160.079 kb on - strand at 2160.079 kb on - strand at 2160.079 kb on - strand at 2160.079 kb on - strand at 2160.079 kb on - strand at 2160.079 kb on - strand at 2160.147 kb on + strand at 2160.147 kb on + strand at 2160.147 kb on + strand at 2160.599 kb on + strand, within queA at 2160.600 kb on - strand, within queA at 2160.600 kb on - strand, within queA at 2160.711 kb on - strand, within queA at 2160.711 kb on - strand, within queA at 2160.749 kb on + strand, within queA at 2160.749 kb on + strand, within queA at 2160.749 kb on + strand, within queA at 2160.749 kb on + strand, within queA at 2160.750 kb on - strand, within queA at 2160.750 kb on - strand, within queA at 2160.750 kb on - strand, within queA at 2160.750 kb on - strand, within queA at 2160.993 kb on - strand, within queA
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Metal_ion_mix_sitestressplate 2 x remove 2,158,626 - yajC OKGIIK_09995 0.69 -1.1 2,158,626 - yajC OKGIIK_09995 0.69 -2.0 2,158,626 - yajC OKGIIK_09995 0.69 -1.9 2,158,626 - yajC OKGIIK_09995 0.69 -1.5 2,158,626 - yajC OKGIIK_09995 0.69 -1.3 2,158,626 - yajC OKGIIK_09995 0.69 -0.5 2,158,626 - yajC OKGIIK_09995 0.69 -0.7 2,158,746 - +0.0 2,158,746 - +0.9 2,158,796 - -0.2 2,158,796 - +0.1 2,158,895 + +0.7 2,158,896 - -0.0 2,158,925 + -0.3 2,158,925 + -0.3 2,158,925 + -0.1 2,158,925 + -0.1 2,158,926 - -0.1 2,158,926 - -0.7 2,158,926 - -0.7 2,158,926 - -0.1 2,158,997 + -0.0 2,158,998 - +1.7 2,158,998 - -1.8 2,159,000 + -0.1 2,159,001 - -1.8 2,159,001 - +1.7 2,159,069 + tgt OKGIIK_10000 0.15 -0.9 2,159,069 + tgt OKGIIK_10000 0.15 +0.8 2,159,099 + tgt OKGIIK_10000 0.18 -1.1 2,159,099 + tgt OKGIIK_10000 0.18 -2.3 2,159,099 + tgt OKGIIK_10000 0.18 -1.4 2,159,100 - tgt OKGIIK_10000 0.18 +0.1 2,159,141 + tgt OKGIIK_10000 0.22 -1.8 2,159,141 + tgt OKGIIK_10000 0.22 +1.3 2,159,141 + tgt OKGIIK_10000 0.22 +0.9 2,159,141 + tgt OKGIIK_10000 0.22 +0.7 2,159,141 + tgt OKGIIK_10000 0.22 -1.1 2,159,141 + tgt OKGIIK_10000 0.22 -0.9 2,159,142 - tgt OKGIIK_10000 0.22 +1.4 2,159,142 - tgt OKGIIK_10000 0.22 -1.7 2,159,142 - tgt OKGIIK_10000 0.22 -2.6 2,159,142 - tgt OKGIIK_10000 0.22 -1.1 2,159,142 - tgt OKGIIK_10000 0.22 -2.3 2,159,288 + tgt OKGIIK_10000 0.34 -1.1 2,159,288 + tgt OKGIIK_10000 0.34 +0.9 2,159,288 + tgt OKGIIK_10000 0.34 -0.2 2,159,288 + tgt OKGIIK_10000 0.34 -1.8 2,159,289 - tgt OKGIIK_10000 0.35 +0.7 2,159,385 - tgt OKGIIK_10000 0.43 +0.7 2,159,385 - tgt OKGIIK_10000 0.43 -3.2 2,159,385 - tgt OKGIIK_10000 0.43 -1.7 2,159,441 + tgt OKGIIK_10000 0.48 -0.6 2,159,441 + tgt OKGIIK_10000 0.48 +0.1 2,159,441 + tgt OKGIIK_10000 0.48 -0.3 2,159,441 + tgt OKGIIK_10000 0.48 -1.7 2,159,442 - tgt OKGIIK_10000 0.48 -0.1 2,159,442 - tgt OKGIIK_10000 0.48 -0.7 2,159,924 + -0.3 2,159,924 + -0.9 2,159,925 - +0.6 2,159,925 - +0.1 2,159,925 - +0.9 2,159,925 - +0.7 2,159,925 - -1.5 2,160,079 - -1.0 2,160,079 - -0.3 2,160,079 - -0.3 2,160,079 - +0.5 2,160,079 - +0.7 2,160,079 - +1.1 2,160,079 - -2.2 2,160,079 - -2.0 2,160,079 - -0.7 2,160,079 - +1.7 2,160,147 + +0.5 2,160,147 + -0.3 2,160,147 + +1.7 2,160,599 + queA OKGIIK_10005 0.41 -0.4 2,160,600 - queA OKGIIK_10005 0.41 +0.2 2,160,600 - queA OKGIIK_10005 0.41 -0.7 2,160,711 - queA OKGIIK_10005 0.51 -0.6 2,160,711 - queA OKGIIK_10005 0.51 +0.2 2,160,749 + queA OKGIIK_10005 0.55 +0.3 2,160,749 + queA OKGIIK_10005 0.55 +0.3 2,160,749 + queA OKGIIK_10005 0.55 -1.5 2,160,749 + queA OKGIIK_10005 0.55 +0.9 2,160,750 - queA OKGIIK_10005 0.55 -0.1 2,160,750 - queA OKGIIK_10005 0.55 -0.1 2,160,750 - queA OKGIIK_10005 0.55 +0.1 2,160,750 - queA OKGIIK_10005 0.55 -0.3 2,160,993 - queA OKGIIK_10005 0.78 +0.8
Or see this region's nucleotide sequence