Experiment: R2A_PIPES with Metal_ion_mix_sitestressplate 2 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tig and Leu_trna are separated by 92 nucleotides Leu_trna and Lys_trna are separated by 274 nucleotides Lys_trna and His_trna are separated by 20 nucleotides His_trna and Arg_trna are separated by 13 nucleotides Arg_trna and Pro_trna are separated by 24 nucleotides Pro_trna and Arg_trna are separated by 177 nucleotides Arg_trna and ftsH are separated by 234 nucleotides
OKGIIK_08045: tig - trigger factor, at 1,776,995 to 1,778,287
tig
OKGIIK_08050: Leu_trna - tRNA-Leu, at 1,778,380 to 1,778,464
_trna
OKGIIK_08055: Lys_trna - tRNA-Lys, at 1,778,739 to 1,778,814
_trna
OKGIIK_08060: His_trna - tRNA-His, at 1,778,835 to 1,778,911
_trna
OKGIIK_08065: Arg_trna - tRNA-Arg, at 1,778,925 to 1,779,001
_trna
OKGIIK_08070: Pro_trna - tRNA-Pro, at 1,779,026 to 1,779,102
_trna
OKGIIK_08075: Arg_trna - tRNA-Arg, at 1,779,280 to 1,779,356
_trna
OKGIIK_08080: ftsH - ATP-dependent zinc metalloprotease FtsH, at 1,779,591 to 1,781,549
ftsH
Position (kb)
1778
1779 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1777.785 kb on + strand, within tig at 1777.786 kb on - strand, within tig at 1777.786 kb on - strand, within tig at 1777.974 kb on + strand, within tig at 1777.974 kb on + strand, within tig at 1777.974 kb on + strand, within tig at 1778.300 kb on + strand at 1778.300 kb on + strand at 1778.300 kb on + strand at 1778.300 kb on + strand at 1778.301 kb on - strand at 1778.301 kb on - strand at 1778.301 kb on - strand at 1778.303 kb on + strand at 1778.303 kb on + strand at 1778.303 kb on + strand at 1778.303 kb on + strand at 1778.303 kb on + strand at 1778.304 kb on - strand at 1778.304 kb on - strand at 1778.304 kb on - strand at 1778.304 kb on - strand at 1778.304 kb on - strand at 1778.304 kb on - strand at 1778.304 kb on - strand at 1778.304 kb on - strand at 1778.304 kb on - strand at 1778.304 kb on - strand at 1778.304 kb on - strand at 1778.361 kb on + strand at 1778.361 kb on + strand at 1778.362 kb on - strand at 1778.362 kb on - strand at 1778.486 kb on - strand at 1778.486 kb on - strand at 1778.486 kb on - strand at 1778.486 kb on - strand at 1778.490 kb on - strand at 1778.490 kb on - strand at 1778.490 kb on - strand at 1778.490 kb on - strand at 1778.490 kb on - strand at 1778.490 kb on - strand at 1778.490 kb on - strand at 1778.490 kb on - strand at 1778.490 kb on - strand at 1778.490 kb on - strand at 1778.490 kb on - strand at 1778.490 kb on - strand at 1778.490 kb on - strand at 1778.490 kb on - strand at 1778.490 kb on - strand at 1778.494 kb on - strand at 1778.494 kb on - strand at 1778.494 kb on - strand at 1778.494 kb on - strand at 1778.494 kb on - strand at 1778.568 kb on - strand at 1778.568 kb on - strand at 1778.568 kb on - strand at 1778.568 kb on - strand at 1778.568 kb on - strand at 1778.568 kb on - strand at 1778.568 kb on - strand at 1778.568 kb on - strand at 1778.568 kb on - strand at 1778.584 kb on + strand at 1778.585 kb on - strand at 1778.587 kb on - strand at 1778.587 kb on - strand at 1778.587 kb on - strand at 1778.587 kb on - strand at 1778.605 kb on - strand at 1778.605 kb on - strand at 1778.605 kb on - strand at 1778.605 kb on - strand at 1778.605 kb on - strand at 1778.923 kb on - strand at 1779.275 kb on - strand at 1779.275 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Metal_ion_mix_sitestressplate 2 x remove 1,777,785 + tig OKGIIK_08045 0.61 -1.3 1,777,786 - tig OKGIIK_08045 0.61 -1.3 1,777,786 - tig OKGIIK_08045 0.61 -1.3 1,777,974 + tig OKGIIK_08045 0.76 -0.0 1,777,974 + tig OKGIIK_08045 0.76 -0.9 1,777,974 + tig OKGIIK_08045 0.76 +1.0 1,778,300 + -1.8 1,778,300 + -0.7 1,778,300 + -0.3 1,778,300 + -0.2 1,778,301 - +2.0 1,778,301 - -0.7 1,778,301 - -0.3 1,778,303 + -0.3 1,778,303 + +1.4 1,778,303 + -0.6 1,778,303 + -1.2 1,778,303 + -1.8 1,778,304 - -0.4 1,778,304 - +0.7 1,778,304 - +0.5 1,778,304 - -0.7 1,778,304 - +0.6 1,778,304 - -0.9 1,778,304 - -0.9 1,778,304 - -1.8 1,778,304 - +1.7 1,778,304 - +1.1 1,778,304 - -0.9 1,778,361 + -0.3 1,778,361 + +2.2 1,778,362 - -0.3 1,778,362 - +0.2 1,778,486 - -0.5 1,778,486 - +0.2 1,778,486 - -0.3 1,778,486 - +0.7 1,778,490 - -0.1 1,778,490 - -2.3 1,778,490 - -1.5 1,778,490 - +0.1 1,778,490 - -0.3 1,778,490 - +0.3 1,778,490 - -1.6 1,778,490 - -1.5 1,778,490 - +1.7 1,778,490 - -0.8 1,778,490 - +0.1 1,778,490 - -0.2 1,778,490 - +0.5 1,778,490 - -0.3 1,778,490 - +0.3 1,778,494 - +1.4 1,778,494 - +0.7 1,778,494 - -0.1 1,778,494 - -2.2 1,778,494 - -0.7 1,778,568 - +1.2 1,778,568 - +0.1 1,778,568 - -0.3 1,778,568 - +0.3 1,778,568 - -0.2 1,778,568 - +0.7 1,778,568 - +0.1 1,778,568 - +0.3 1,778,568 - -0.4 1,778,584 + -0.3 1,778,585 - -1.9 1,778,587 - -1.9 1,778,587 - +0.7 1,778,587 - +2.0 1,778,587 - +0.8 1,778,605 - -2.9 1,778,605 - -0.7 1,778,605 - -2.2 1,778,605 - -0.8 1,778,605 - -0.7 1,778,923 - -0.5 1,779,275 - -0.5 1,779,275 - +0.2
Or see this region's nucleotide sequence