Experiment: R2A_PIPES with Metal_ion_mix_sitestressplate 2 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKGIIK_02620 and OKGIIK_02625 are separated by 108 nucleotides OKGIIK_02625 and flgM overlap by 4 nucleotides flgM and flgA are separated by 109 nucleotides flgA and OKGIIK_02640 overlap by 4 nucleotides
OKGIIK_02620: OKGIIK_02620 - Diguanylate phosphodiesterase, at 611,715 to 613,007
_02620
OKGIIK_02625: OKGIIK_02625 - Flagellar biosynthesis protein FlgN, at 613,116 to 613,562
_02625
OKGIIK_02630: flgM - flagellar biosynthesis anti-sigma factor FlgM, at 613,559 to 613,882
flgM
OKGIIK_02635: flgA - flagellar basal body P-ring formation protein FlgA, at 613,992 to 614,699
flgA
OKGIIK_02640: OKGIIK_02640 - Chemotaxis protein, at 614,696 to 616,000
_02640
Position (kb) 613
614 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 612.670 kb on + strand, within OKGIIK_02620 at 612.671 kb on - strand, within OKGIIK_02620 at 612.771 kb on + strand, within OKGIIK_02620 at 612.772 kb on - strand, within OKGIIK_02620 at 612.772 kb on - strand, within OKGIIK_02620 at 612.866 kb on - strand, within OKGIIK_02620 at 612.866 kb on - strand, within OKGIIK_02620 at 612.940 kb on + strand at 612.940 kb on + strand at 612.940 kb on + strand at 612.940 kb on + strand at 612.940 kb on + strand at 612.940 kb on + strand at 612.941 kb on - strand at 612.941 kb on - strand at 612.941 kb on - strand at 612.941 kb on - strand at 612.941 kb on - strand at 612.999 kb on + strand at 612.999 kb on + strand at 612.999 kb on + strand at 612.999 kb on + strand at 613.000 kb on - strand at 613.000 kb on - strand at 613.028 kb on + strand at 613.065 kb on + strand at 613.065 kb on + strand at 613.065 kb on + strand at 613.066 kb on - strand at 613.066 kb on - strand at 613.066 kb on - strand at 613.066 kb on - strand at 613.066 kb on - strand at 613.174 kb on + strand, within OKGIIK_02625 at 613.174 kb on + strand, within OKGIIK_02625 at 613.174 kb on + strand, within OKGIIK_02625 at 613.175 kb on - strand, within OKGIIK_02625 at 613.175 kb on - strand, within OKGIIK_02625 at 613.224 kb on + strand, within OKGIIK_02625 at 613.224 kb on + strand, within OKGIIK_02625 at 613.224 kb on + strand, within OKGIIK_02625 at 613.224 kb on + strand, within OKGIIK_02625 at 613.224 kb on + strand, within OKGIIK_02625 at 613.225 kb on - strand, within OKGIIK_02625 at 613.776 kb on - strand, within flgM at 613.913 kb on - strand at 613.913 kb on - strand at 613.913 kb on - strand at 613.945 kb on + strand at 613.945 kb on + strand at 613.945 kb on + strand at 613.945 kb on + strand at 613.945 kb on + strand at 613.946 kb on - strand at 613.946 kb on - strand at 613.946 kb on - strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.961 kb on - strand at 613.961 kb on - strand at 613.961 kb on - strand at 613.961 kb on - strand at 613.961 kb on - strand at 613.961 kb on - strand at 613.961 kb on - strand at 613.961 kb on - strand at 613.961 kb on - strand at 613.961 kb on - strand at 613.961 kb on - strand at 613.961 kb on - strand at 613.961 kb on - strand at 613.961 kb on - strand at 613.961 kb on - strand at 613.961 kb on - strand at 613.961 kb on - strand at 613.962 kb on + strand at 613.962 kb on + strand at 613.962 kb on + strand at 613.963 kb on - strand at 613.963 kb on - strand at 613.963 kb on - strand at 614.269 kb on + strand, within flgA at 614.269 kb on + strand, within flgA at 614.567 kb on - strand, within flgA at 614.567 kb on - strand, within flgA at 614.567 kb on - strand, within flgA at 614.567 kb on - strand, within flgA at 614.567 kb on - strand, within flgA
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Metal_ion_mix_sitestressplate 2 x remove 612,670 + OKGIIK_02620 0.74 -1.7 612,671 - OKGIIK_02620 0.74 -0.4 612,771 + OKGIIK_02620 0.82 +0.7 612,772 - OKGIIK_02620 0.82 -0.9 612,772 - OKGIIK_02620 0.82 -0.9 612,866 - OKGIIK_02620 0.89 +0.0 612,866 - OKGIIK_02620 0.89 +0.6 612,940 + -0.7 612,940 + -0.7 612,940 + -1.1 612,940 + +1.8 612,940 + +1.3 612,940 + +0.3 612,941 - +1.4 612,941 - -1.4 612,941 - +0.8 612,941 - -0.7 612,941 - -0.5 612,999 + +0.5 612,999 + -0.4 612,999 + +1.6 612,999 + -1.0 613,000 - -0.2 613,000 - -2.1 613,028 + -0.2 613,065 + -1.4 613,065 + +0.3 613,065 + -3.1 613,066 - +1.6 613,066 - +0.4 613,066 - -0.7 613,066 - +1.1 613,066 - -1.7 613,174 + OKGIIK_02625 0.13 +2.2 613,174 + OKGIIK_02625 0.13 -0.1 613,174 + OKGIIK_02625 0.13 -0.4 613,175 - OKGIIK_02625 0.13 +0.3 613,175 - OKGIIK_02625 0.13 -0.2 613,224 + OKGIIK_02625 0.24 +0.2 613,224 + OKGIIK_02625 0.24 -1.2 613,224 + OKGIIK_02625 0.24 -1.7 613,224 + OKGIIK_02625 0.24 +0.0 613,224 + OKGIIK_02625 0.24 +0.5 613,225 - OKGIIK_02625 0.24 +0.9 613,776 - flgM OKGIIK_02630 0.67 -2.9 613,913 - -0.7 613,913 - -1.4 613,913 - -0.0 613,945 + -0.7 613,945 + +0.0 613,945 + -0.9 613,945 + +0.3 613,945 + -1.2 613,946 - -0.7 613,946 - +0.1 613,946 - +1.2 613,960 + -0.2 613,960 + -2.2 613,960 + -0.4 613,960 + -0.6 613,960 + -1.2 613,960 + +1.6 613,960 + +0.6 613,960 + -2.5 613,960 + +0.0 613,960 + +0.3 613,960 + -1.6 613,960 + +0.8 613,960 + +0.1 613,960 + +0.6 613,960 + -2.0 613,960 + +0.6 613,960 + +0.2 613,960 + +1.1 613,960 + +0.7 613,960 + -0.1 613,960 + -0.3 613,961 - -1.0 613,961 - -0.9 613,961 - -0.7 613,961 - +1.5 613,961 - -0.5 613,961 - +0.1 613,961 - +0.3 613,961 - -0.6 613,961 - -2.0 613,961 - +0.6 613,961 - +0.4 613,961 - -1.3 613,961 - -1.7 613,961 - -0.9 613,961 - +0.0 613,961 - +0.0 613,961 - -0.7 613,962 + +0.3 613,962 + -0.2 613,962 + -1.5 613,963 - -0.8 613,963 - +0.2 613,963 - -0.9 614,269 + flgA OKGIIK_02635 0.39 +1.1 614,269 + flgA OKGIIK_02635 0.39 -0.4 614,567 - flgA OKGIIK_02635 0.81 +0.0 614,567 - flgA OKGIIK_02635 0.81 +0.1 614,567 - flgA OKGIIK_02635 0.81 +0.7 614,567 - flgA OKGIIK_02635 0.81 -1.1 614,567 - flgA OKGIIK_02635 0.81 -0.6
Or see this region's nucleotide sequence