Experiment: R2A_PIPES with Metal_ion_mix_sitestressplate 2 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hemN and crp are separated by 32 nucleotides crp and OKGIIK_00810 are separated by 30 nucleotides OKGIIK_00810 and OKGIIK_00815 are separated by 33 nucleotides OKGIIK_00815 and OKGIIK_00820 are separated by 141 nucleotides
OKGIIK_00800: hemN - oxygen-independent coproporphyrinogen III oxidase, at 177,712 to 179,112
hemN
OKGIIK_00805: crp - cAMP-binding domain of CRP or a regulatory subunit of cAMP-dependent protein kinases, at 179,145 to 179,906
crp
OKGIIK_00810: OKGIIK_00810 - Universal stress protein UspA, at 179,937 to 180,782
_00810
OKGIIK_00815: OKGIIK_00815 - GNAT family acetyltransferase, at 180,816 to 181,388
_00815
OKGIIK_00820: OKGIIK_00820 - CRP-like protein Clp, at 181,530 to 182,294
_00820
Position (kb)
179
180
181 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 179.040 kb on + strand at 179.040 kb on + strand at 179.041 kb on - strand at 179.041 kb on - strand at 179.041 kb on - strand at 179.041 kb on - strand at 179.065 kb on + strand at 179.065 kb on + strand at 179.065 kb on + strand at 179.065 kb on + strand at 179.065 kb on + strand at 179.065 kb on + strand at 179.066 kb on - strand at 179.066 kb on - strand at 179.066 kb on - strand at 179.066 kb on - strand at 179.066 kb on - strand at 179.095 kb on + strand at 179.095 kb on + strand at 179.157 kb on + strand at 179.158 kb on - strand at 179.228 kb on + strand, within crp at 179.228 kb on + strand, within crp at 179.229 kb on - strand, within crp at 179.229 kb on - strand, within crp at 179.229 kb on - strand, within crp at 179.229 kb on - strand, within crp at 179.442 kb on + strand, within crp at 179.442 kb on + strand, within crp at 179.442 kb on + strand, within crp at 179.443 kb on - strand, within crp at 179.443 kb on - strand, within crp at 179.443 kb on - strand, within crp at 179.443 kb on - strand, within crp at 179.443 kb on - strand, within crp at 179.478 kb on + strand, within crp at 179.478 kb on + strand, within crp at 179.479 kb on - strand, within crp at 179.698 kb on - strand, within crp at 179.763 kb on + strand, within crp at 179.763 kb on + strand, within crp at 179.763 kb on + strand, within crp at 179.763 kb on + strand, within crp at 179.763 kb on + strand, within crp at 179.763 kb on + strand, within crp at 179.763 kb on + strand, within crp at 179.763 kb on + strand, within crp at 179.763 kb on + strand, within crp at 179.764 kb on - strand, within crp at 179.764 kb on - strand, within crp at 179.764 kb on - strand, within crp at 179.764 kb on - strand, within crp at 179.764 kb on - strand, within crp at 179.764 kb on - strand, within crp at 179.764 kb on - strand, within crp at 179.905 kb on - strand at 180.028 kb on + strand, within OKGIIK_00810 at 180.115 kb on + strand, within OKGIIK_00810 at 180.115 kb on + strand, within OKGIIK_00810 at 180.115 kb on + strand, within OKGIIK_00810 at 180.115 kb on + strand, within OKGIIK_00810 at 180.115 kb on + strand, within OKGIIK_00810 at 180.115 kb on + strand, within OKGIIK_00810 at 180.115 kb on + strand, within OKGIIK_00810 at 180.115 kb on + strand, within OKGIIK_00810 at 180.115 kb on + strand, within OKGIIK_00810 at 180.115 kb on + strand, within OKGIIK_00810 at 180.115 kb on + strand, within OKGIIK_00810 at 180.115 kb on + strand, within OKGIIK_00810 at 180.116 kb on - strand, within OKGIIK_00810 at 180.116 kb on - strand, within OKGIIK_00810 at 180.116 kb on - strand, within OKGIIK_00810 at 180.116 kb on - strand, within OKGIIK_00810 at 180.152 kb on - strand, within OKGIIK_00810 at 180.883 kb on + strand, within OKGIIK_00815 at 180.883 kb on + strand, within OKGIIK_00815 at 180.883 kb on + strand, within OKGIIK_00815 at 180.884 kb on - strand, within OKGIIK_00815 at 180.884 kb on - strand, within OKGIIK_00815 at 180.884 kb on - strand, within OKGIIK_00815 at 181.066 kb on + strand, within OKGIIK_00815 at 181.066 kb on + strand, within OKGIIK_00815 at 181.067 kb on - strand, within OKGIIK_00815 at 181.067 kb on - strand, within OKGIIK_00815 at 181.278 kb on + strand, within OKGIIK_00815 at 181.278 kb on + strand, within OKGIIK_00815 at 181.279 kb on - strand, within OKGIIK_00815 at 181.279 kb on - strand, within OKGIIK_00815 at 181.279 kb on - strand, within OKGIIK_00815 at 181.279 kb on - strand, within OKGIIK_00815 at 181.285 kb on - strand, within OKGIIK_00815 at 181.477 kb on + strand at 181.477 kb on + strand at 181.477 kb on + strand at 181.477 kb on + strand at 181.477 kb on + strand at 181.477 kb on + strand at 181.478 kb on - strand at 181.478 kb on - strand at 181.478 kb on - strand at 181.478 kb on - strand at 181.478 kb on - strand at 181.635 kb on + strand, within OKGIIK_00820 at 181.635 kb on + strand, within OKGIIK_00820 at 181.636 kb on - strand, within OKGIIK_00820 at 181.636 kb on - strand, within OKGIIK_00820 at 181.696 kb on + strand, within OKGIIK_00820 at 181.696 kb on + strand, within OKGIIK_00820 at 181.696 kb on + strand, within OKGIIK_00820 at 181.696 kb on + strand, within OKGIIK_00820 at 181.697 kb on - strand, within OKGIIK_00820 at 181.697 kb on - strand, within OKGIIK_00820 at 181.697 kb on - strand, within OKGIIK_00820 at 181.697 kb on - strand, within OKGIIK_00820 at 181.712 kb on - strand, within OKGIIK_00820
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Metal_ion_mix_sitestressplate 2 x remove 179,040 + -1.2 179,040 + +1.4 179,041 - -0.8 179,041 - -0.5 179,041 - +1.2 179,041 - +0.5 179,065 + -0.7 179,065 + +0.5 179,065 + -1.4 179,065 + +0.8 179,065 + +0.6 179,065 + -0.0 179,066 - +0.1 179,066 - +0.6 179,066 - +0.2 179,066 - -0.4 179,066 - -1.7 179,095 + +1.0 179,095 + -0.4 179,157 + +2.2 179,158 - -0.7 179,228 + crp OKGIIK_00805 0.11 -2.1 179,228 + crp OKGIIK_00805 0.11 +0.3 179,229 - crp OKGIIK_00805 0.11 -0.2 179,229 - crp OKGIIK_00805 0.11 -0.2 179,229 - crp OKGIIK_00805 0.11 -1.3 179,229 - crp OKGIIK_00805 0.11 +1.5 179,442 + crp OKGIIK_00805 0.39 +1.2 179,442 + crp OKGIIK_00805 0.39 -1.7 179,442 + crp OKGIIK_00805 0.39 -0.7 179,443 - crp OKGIIK_00805 0.39 -0.5 179,443 - crp OKGIIK_00805 0.39 -0.7 179,443 - crp OKGIIK_00805 0.39 -0.4 179,443 - crp OKGIIK_00805 0.39 -1.7 179,443 - crp OKGIIK_00805 0.39 +1.3 179,478 + crp OKGIIK_00805 0.44 +2.4 179,478 + crp OKGIIK_00805 0.44 -1.5 179,479 - crp OKGIIK_00805 0.44 +0.6 179,698 - crp OKGIIK_00805 0.73 -0.2 179,763 + crp OKGIIK_00805 0.81 -1.4 179,763 + crp OKGIIK_00805 0.81 -0.5 179,763 + crp OKGIIK_00805 0.81 +0.1 179,763 + crp OKGIIK_00805 0.81 +0.1 179,763 + crp OKGIIK_00805 0.81 -0.5 179,763 + crp OKGIIK_00805 0.81 -0.7 179,763 + crp OKGIIK_00805 0.81 +0.9 179,763 + crp OKGIIK_00805 0.81 -1.9 179,763 + crp OKGIIK_00805 0.81 +0.6 179,764 - crp OKGIIK_00805 0.81 +0.9 179,764 - crp OKGIIK_00805 0.81 -1.9 179,764 - crp OKGIIK_00805 0.81 -1.5 179,764 - crp OKGIIK_00805 0.81 -1.7 179,764 - crp OKGIIK_00805 0.81 -0.7 179,764 - crp OKGIIK_00805 0.81 +0.1 179,764 - crp OKGIIK_00805 0.81 -0.1 179,905 - -0.8 180,028 + OKGIIK_00810 0.11 -1.2 180,115 + OKGIIK_00810 0.21 -2.1 180,115 + OKGIIK_00810 0.21 -2.0 180,115 + OKGIIK_00810 0.21 +0.1 180,115 + OKGIIK_00810 0.21 +0.9 180,115 + OKGIIK_00810 0.21 -0.4 180,115 + OKGIIK_00810 0.21 +1.8 180,115 + OKGIIK_00810 0.21 -0.4 180,115 + OKGIIK_00810 0.21 +0.6 180,115 + OKGIIK_00810 0.21 -2.0 180,115 + OKGIIK_00810 0.21 +0.3 180,115 + OKGIIK_00810 0.21 +0.1 180,115 + OKGIIK_00810 0.21 +0.4 180,116 - OKGIIK_00810 0.21 -0.5 180,116 - OKGIIK_00810 0.21 -0.4 180,116 - OKGIIK_00810 0.21 +0.4 180,116 - OKGIIK_00810 0.21 +1.5 180,152 - OKGIIK_00810 0.25 +0.3 180,883 + OKGIIK_00815 0.12 +1.6 180,883 + OKGIIK_00815 0.12 -0.3 180,883 + OKGIIK_00815 0.12 +0.5 180,884 - OKGIIK_00815 0.12 +1.0 180,884 - OKGIIK_00815 0.12 -0.9 180,884 - OKGIIK_00815 0.12 -0.8 181,066 + OKGIIK_00815 0.44 -0.4 181,066 + OKGIIK_00815 0.44 +1.1 181,067 - OKGIIK_00815 0.44 -1.2 181,067 - OKGIIK_00815 0.44 +0.2 181,278 + OKGIIK_00815 0.81 -1.9 181,278 + OKGIIK_00815 0.81 -0.2 181,279 - OKGIIK_00815 0.81 -2.4 181,279 - OKGIIK_00815 0.81 -0.7 181,279 - OKGIIK_00815 0.81 +1.6 181,279 - OKGIIK_00815 0.81 +0.6 181,285 - OKGIIK_00815 0.82 -0.4 181,477 + -1.7 181,477 + +2.0 181,477 + -0.0 181,477 + -0.8 181,477 + -0.6 181,477 + -0.6 181,478 - -0.3 181,478 - -0.4 181,478 - +0.4 181,478 - -0.5 181,478 - -0.7 181,635 + OKGIIK_00820 0.14 -0.8 181,635 + OKGIIK_00820 0.14 -1.7 181,636 - OKGIIK_00820 0.14 +0.6 181,636 - OKGIIK_00820 0.14 -0.1 181,696 + OKGIIK_00820 0.22 -1.8 181,696 + OKGIIK_00820 0.22 +0.9 181,696 + OKGIIK_00820 0.22 +0.8 181,696 + OKGIIK_00820 0.22 -0.5 181,697 - OKGIIK_00820 0.22 +0.1 181,697 - OKGIIK_00820 0.22 +1.3 181,697 - OKGIIK_00820 0.22 -0.7 181,697 - OKGIIK_00820 0.22 -0.4 181,712 - OKGIIK_00820 0.24 -0.7
Or see this region's nucleotide sequence