Experiment: D,L-Lactate and casaminos (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3657 and GFF3658 are separated by 7 nucleotides GFF3658 and GFF3659 are separated by 85 nucleotides
GFF3657 - hypothetical protein, at 245,474 to 246,865
GFF3657
GFF3658 - D-beta-hydroxybutyrate dehydrogenase, at 246,873 to 247,646
GFF3658
GFF3659 - Acetyl-coenzyme A synthetase, at 247,732 to 249,687
GFF3659
Position (kb)
246
247
248 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 245.878 kb on + strand, within GFF3657 at 245.878 kb on + strand, within GFF3657 at 245.878 kb on + strand, within GFF3657 at 245.878 kb on + strand, within GFF3657 at 245.879 kb on - strand, within GFF3657 at 246.289 kb on + strand, within GFF3657 at 246.658 kb on + strand, within GFF3657 at 246.779 kb on + strand at 246.779 kb on + strand at 246.780 kb on - strand at 246.799 kb on + strand at 246.799 kb on + strand at 246.799 kb on + strand at 246.800 kb on - strand at 246.800 kb on - strand at 246.800 kb on - strand at 246.800 kb on - strand at 246.846 kb on - strand at 246.846 kb on - strand at 246.869 kb on - strand at 246.869 kb on - strand at 247.092 kb on + strand, within GFF3658 at 247.329 kb on + strand, within GFF3658 at 247.329 kb on + strand, within GFF3658 at 247.329 kb on + strand, within GFF3658 at 247.330 kb on - strand, within GFF3658 at 247.619 kb on + strand at 247.620 kb on - strand at 247.645 kb on - strand at 247.685 kb on + strand at 247.685 kb on + strand at 247.685 kb on + strand at 247.686 kb on - strand at 247.686 kb on - strand at 247.715 kb on + strand at 247.715 kb on + strand at 247.716 kb on - strand at 247.716 kb on - strand at 247.716 kb on - strand at 247.716 kb on - strand at 247.824 kb on + strand at 248.318 kb on - strand, within GFF3659 at 248.410 kb on + strand, within GFF3659 at 248.479 kb on + strand, within GFF3659 at 248.533 kb on + strand, within GFF3659 at 248.533 kb on + strand, within GFF3659 at 248.533 kb on + strand, within GFF3659 at 248.534 kb on - strand, within GFF3659
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate and casaminos (C) remove 245,878 + GFF3657 0.29 -0.6 245,878 + GFF3657 0.29 -0.0 245,878 + GFF3657 0.29 -0.6 245,878 + GFF3657 0.29 +0.2 245,879 - GFF3657 0.29 -0.1 246,289 + GFF3657 0.59 -0.4 246,658 + GFF3657 0.85 -1.0 246,779 + +0.8 246,779 + +0.4 246,780 - -0.9 246,799 + +0.1 246,799 + -1.5 246,799 + -0.1 246,800 - +1.6 246,800 - -0.4 246,800 - -0.6 246,800 - +0.0 246,846 - -0.9 246,846 - +0.4 246,869 - -0.1 246,869 - -0.6 247,092 + GFF3658 0.28 -0.7 247,329 + GFF3658 0.59 -0.3 247,329 + GFF3658 0.59 -0.8 247,329 + GFF3658 0.59 -0.2 247,330 - GFF3658 0.59 -1.0 247,619 + -0.2 247,620 - +0.6 247,645 - -0.3 247,685 + +1.8 247,685 + -0.6 247,685 + +0.1 247,686 - -1.0 247,686 - -0.4 247,715 + -0.3 247,715 + -0.2 247,716 - -0.4 247,716 - -0.9 247,716 - -1.2 247,716 - +0.4 247,824 + +1.4 248,318 - GFF3659 0.30 +0.4 248,410 + GFF3659 0.35 -1.8 248,479 + GFF3659 0.38 -0.2 248,533 + GFF3659 0.41 +0.7 248,533 + GFF3659 0.41 +0.2 248,533 + GFF3659 0.41 +0.4 248,534 - GFF3659 0.41 -0.1
Or see this region's nucleotide sequence